Bacteriophages for the control of bacterial speck disease
Agricultural compositions are disclosed which comprise at least one isolated bacteriophage capable of infecting the plant pathogen Pseudomonas syringae pv. tomato, the at least one bacteriophage having a genomic nucleic acid sequence at least 85% identical to one of the nucleic acid sequence as set forth in SEQ ID NOs: 1-23. The composition comprises no more than 10 different strains of bacteriophage. Uses thereof for treating bacterial speck disease are also disclosed.
1 . An agricultural composition comprising:
(i) at least three non-identical isolated bacteriophages capable of infecting the plant pathogen Pseudomonas syringae pv. tomato, said at least three non-identical bacteriophages having the genomic nucleic acid sequences as set forth in SEQ ID NOs: 17, 19, and 21 respectively; and
(ii) an agriculturally acceptable carrier, wherein the composition comprises no more than 10 different strains of bacteriophage.
2 . The agricultural composition of claim 1 , wherein the composition comprises no additional bacteriophage that infects plant pathogens other than Pseudomonas syringae pv. tomato.
3 . The agricultural composition of claim 1 , further comprising an agent which promotes the growth of a plant or maintains the health of the plant.
4 . The agricultural composition of claim 3 , wherein said agent is selected from the group consisting of a fertilizer, an acaricide, a herbicide, a fungicide, an insecticide, a nematicide, a pesticide, a plant growth regulator, a rodenticide and a nutrient.
5 . The agricultural composition of claim 1 , wherein said agriculturally acceptable carrier comprises at least one agent selected from the group consisting of a stabilizer, a tackifier, a preservative, a carrier, a surfactant, an anticomplex agent and a combination thereof.
6 . An article of manufacture comprising:
(i) a first bacteriophage capable of infecting the pathogen Pseudomonas syringae pv. tomato, the bacteriophage having the genomic nucleic acid sequence as set forth in SEQ ID NO: 19;
(ii) a second, non-identical bacteriophage capable of infecting the pathogen Pseudomonas syringae pv. tomato, the bacteriophage having the genomic nucleic acid sequence as set forth in SEQ ID NO: 17; and
(iii) a third, non-identical bacteriophage capable of infecting the pathogen Pseudomonas syringae pv. tomato, the bacteriophage having the genomic nucleic acid sequence as set forth in SEO ID NO: 21.
7 . A method of controlling or preventing bacterial speck disease of a tomato plant comprising contacting the tomato plant with at least three non-identical bacteriophages in an amount which is effective at infecting the plant pathogen Pseudomonas syringae pv. tomato, said at least three non-identical bacteriophages having the genomic nucleic acid sequence as set forth in SEQ ID NOs: 19 and 21, thereby controlling or preventing the bacterial speck disease of the tomato plant.
8 . The method of claim 7 , wherein said contacting is affected at a seedling phase.
9 . The method of claim 7 , wherein said contacting is in the soil in the vicinity of or onto: a root, a stem, a seed, a fruit, a flower, a leaf, or any combination thereof.
10 . The method of claim 7 , wherein said contacting comprises dipping, spraying or fogging.