Granted Patent
B2
US 12,594,557 · App. 18/318,294 · Granted Apr 7, 2026
Isoelectric focusing devices and fixtures
Inventors:
Luc Bousse (Los Altos, CA); Don Wesley Arnold (Livermore, CA); Wesley Chang (Fremont, CA); Erik Gentalen (Fremont, CA); Eric Gwerder (Pleasanton, CA); Scott Mack (Boulder Creek, CA)
Assignee:
Intabio, LLC
B01L3/502776B01L3/5027B01L3/502715B01L3/502761B01L3/502792G01N27/44791G01N27/44795B01L2200/0636B01L2200/0647B01L2200/0684B01L2300/047B01L2300/0645B01L2300/0816B01L2300/0864B01L2300/12B01L2300/161B01L2400/0421B01L2400/0427
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Abstract
Methods, devices, and systems for performing isoelectric focusing reactions are described. The systems or devices disclosed herein may comprise fixtures that have a membrane. In some instances, the disclosed devices may be designed to perform isoelectric focusing or other separation reactions followed by further characterization of the separated analytes using mass spectrometry. Two or more isoelectric focusing reactions may be performed in parallel. The disclosed methods, devices, and systems provide for fast, accurate separation and characterization of protein analyte mixtures or other biological molecules by isoelectric point.
Claims (29)
1 . A system comprising:
a fluidic device, wherein the fluidic device comprises
at least one fluid inlet,
at least one fluid outlet,
at least one separation channel comprising a first end that is fluidically coupled to the at least one fluid inlet and a second end that is fluidically coupled to the at least one fluid outlet,
and a mobilizer channel that intersects with a second end of the separation channel, and
a nebulizer, wherein the nebulizer is configured to surround at least part of the fluidic device on at least four sides and wherein the fluidic device is polygonal,
wherein a gap between the nebulizer and the fluidic device is formed, and
wherein the nebulizer comprises a chamber and an inlet that directs nebulizing gas inside the gap.
2 . The system of claim 1 , wherein the gap is less than about 500 microns.
3 . The system of claim 2 , wherein the gap is less than about 200 microns.
4 . The system of claim 1 , wherein the nebulizer further comprises at least one slit to direct the nebulizing gas out of the nebulizer.
5 . The system of claim 1 , wherein the nebulizing gas has a velocity of greater than about 10 m/s.
6 . The system of claim 5 , wherein the nebulizing gas has a velocity of greater than about 30 m/s.
7 . The system of claim 6 , wherein the nebulizing gas has a velocity of greater than about 100 m/s.
8 . The system of claim 1 , wherein the pressure of the nebulizing gas is lower than about 40 psi.
9 . The system of claim 1 , wherein the nebulizing gas surrounds the fluid outlet.
10 . The system of claim 1 , wherein the nebulizing gas is an inert gas.
11 . The system of claim 1 , wherein the nebulizer may comprise at least one fastener to secure the nebulizer to the fluidic device.
12 . The system of claim 1 , wherein the system further comprises a catholyte channel.
13 . The system of claim 1 , wherein the system further comprises an anolyte channel.
14 . A method for performing isoelectric focusing comprising:
introducing a sample into a system of claim 1 ;
applying an electric field across the separation channel to separate the sample via isoelectric focusing and generate focused sample peaks;
mobilizing the focused sample peaks; and
expelling the mobilized sample peaks via electrospray ionization into a mass spectrometer from an orifice, wherein the nebulizing gas surrounds the orifice.
15 . The method of claim 14 , wherein the orifice is a single orifice.
16 . The method of claim 14 , further comprising imaging the separation channel or a portion thereof during or after the isoelectric focusing separation and/or mobilization.
17 . The method of claim 14 , further comprising correlating the sample peaks detected by imaging of the separation channel or a portion thereof with mass spectrometry data for the separated sample.
Assignments (4)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Mar 18, 2026
From: CHANG, WESLEY
To: INTABIO, LLC
Reel/Frame 074117/0131 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Mar 2, 2026
From: BOUSSE, LUC; GENTALEN, ERIK; GWERDER, ERIC; MACK, SCOTT
To: INTABIO, INC.
Reel/Frame 073944/0670 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Mar 2, 2026
From: INTABIO, INC.
To: INTABIO, LLC
Reel/Frame 073944/0767 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Mar 2, 2026
From: ARNOLD, DON WESLEY
To: INTABIO, INC.
Reel/Frame 073944/0794 →
Continuity (7)
Continuation
16808063
· Mar 3, 2020
Provisional Application
62909675
· Oct 2, 2019
Provisional Application
62893549
· Aug 29, 2019
Provisional Application
62885733
· Aug 12, 2019
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