Microorganism of
The present disclosure relates to a Corynebacterium sp. mutant strain having increased L-arginine or L-citrulline productivity and a method of producing L-arginine or L-citrulline using the same. The Corynebacterium sp. mutant strain has enhanced activity of acetylglutamate kinase involved in the L-arginine biosynthesis pathway, and thus is capable of producing L-arginine or L-citrulline in an increased yield compared to a parent strain.
1 . A Corynebacterium sp. mutant strain having increased L-arginine or L-citrulline productivity by having enhanced activity of acetylglutamate kinase,
wherein said acetylglutamate kinase of the Corynebacterium sp. mutant strain having increased L-citrulline productivity has a substitution of aspartic acid for histidine at amino acid position 245 in the amino acid sequence of SEQ ID NO: 2,
wherein said acetylglutamate kinase of the Corynebacterium sp. mutant strain having increased L-arginine productivity has a substitution of leucine for glutamic acid at amino acid position 258 or a substitution of aspartic acid for histidine at amino acid position 245 in the amino acid sequence of SEQ ID NO: 2.
2 . The Corynebacterium sp. mutant strain of claim 1 , which is Corynebacterium glutamicum.
3 . A method for producing L-arginine or L-citrulline comprising steps of:
culturing the Corynebacterium sp. mutant strain of claim 1 in a medium; and
recovering L-arginine or L-citrulline from the cultured mutant strain or the medium in which the mutant strain has been cultured.