Granted Patent
B2
US 12,599,141 · App. 16/306,855 · Granted Apr 14, 2026
Rod-shaped plant viral nanoparticles or virus-like particles for agricultural applications
Assignee:
CASE WESTERN RESERVE UNIVERSITY
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Abstract
An agricultural composition includes a plurality of rod-shaped plant viral nanoparticles (VNPs) and/or virus-like particles (VLPs), each VNP and/or VLP having an exterior surface and an interior surface that extend from a first end to a second of the rod-shaped VNP and/or VLP, the interior surface defining a channel that extends through rod-shaped VNP from the first end to the second end; and at least one agrochemical agent attached or conjugated to the interior and/or exterior surface of the rod-shaped plant VNPs and/or VLPs.
Claims (9)
1 . A method of treating a plant, comprising applying an agricultural composition to the plant in a treatment effective amount to combat nematode parasitism on the plant, the agricultural composition including a plurality of tobacco mild green mosaic virus (TMGMV) and/or VLPs thereof, each TMGMV and/or VLP having an interior surface that extends from a first end to a second end of the TMGMV and/or VLP, the interior surface defining a channel that extends through TMGMV and/or VLPs thereof from the first end to the second end; and crystal violet (hexamethyl parparosaniline chloride) non-covalently loaded onto negatively charged interior surface of the TMGMV and/or VLPs by electrostatic interactions, wherein the negatively charged interior surface of the TMGMV and/or VLPs thereof includes a solvent exposed glutamic acid 95 (Glu95) amino acid residue on the interior surface of the TMGMV and/or VLPs thereof.
2 . The method of claim 1 , wherein the plant is a monocot or dicot.
3 . The method of claim 1 , wherein the plant is selected from the group consisting of wheat, corn (maize), soybean, cotton, cassava, potato, sweet potato, bananas, citrus, strawberries, tomato, coffee, carrots, peppers, turf grass, and greenhouse ornamentals.
4 . The method of claim 1 , wherein the plant is a plant part selected from the group consisting of leaves, flowers, stems, roots, tubers, fruits, and seeds.
5 . The method of claim 1 , wherein the plant is a seed.
6 . The method of claim 1 , wherein the nematode is selected from the group consisting of Meloidogyne root knot nematodes, Globodera and Heterodera cyst nematodes; Pratylenchus lesion nematodies, Dietylenchus stem and bulb nematodes, Tylenchulus citrus nematodes, Xiphinema dagger nematodes, Radopholus burrowing nematodes, Rotylenchulus reniform nematodes, Helicotylenchus spiral nematodes, and Belonolaimus sting nematodes.
7 . The method of claim 1 , wherein the composition is a sprayable composition.
8 . The method of claim 1 , further comprising a water carrier.
9 . The method of claim 1 , wherein the composition is applied as a seed coating.
Assignments (2)
CONFIRMATORY LICENSE
Recorded Mar 26, 2025
From: CASE WESTERN RESERVE UNIVERSITY
To: NATIONAL SCIENCE FOUNDATION
Reel/Frame 070630/0463 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Nov 13, 2019
From: STEINMETZ, NICOLE F.; CHARIOU, PAUL L.
To: CASE WESTERN RESERVE UNIVERSITY
Reel/Frame 051003/0090 →
Continuity (2)
Provisional Application
62345212
· Jun 3, 2016
References Cited (93)
US 5004606A
· Frincke
· 1991
[cited by applicant]
US 5723750A
· Stubbs
· 1998
[cited by examiner]
US 9925281B2
· Steinmetz et al.
· 2018
[cited by applicant]
US 20030181355A1
· Glenn
· 2003
[cited by examiner]
US 20050019270A1
· Finlay et al.
· 2005
[cited by applicant]
US 20070160628A1
· Birkett
· 2007
[cited by examiner]
US 20070258889A1
· Douglas
· 2007
[cited by applicant]
US 20100183504A1
· Chen
· 2010
[cited by applicant]
US 20150033418A1
· Lommel
· 2015
[cited by examiner]
JP 2009524699A
· 2009
[cited by applicant]
WO 0118199A1
· 2001
[cited by applicant]
WO 200118199A1
· 2001
[cited by applicant]
WO 20010026682A2
· 2001
[cited by applicant]
WO 2003092623A2
· 2003
[cited by applicant]
WO 2012078069A1
· 2012
[cited by applicant]
WO WO2013158620A1
· 2013
[cited by examiner]
WO 2013181557A1
· 2013
[cited by applicant]
WO 20150039255A1
· 2015
[cited by applicant]
WO 2016073972A1
· 2016
[cited by applicant]
Love et al. (The use of tobacco mosaic virus and cowpea mosaic virus for the production of novel metal nanomaterials, Virology 449 (2014), pp. 133-139) (Year: 2014).
[cited by examiner]
Miermont et al., “Cowpea Mosaic Virus Capsid: A promising Carrier for the Development of Carbohydrate Based Antitumor Vaccines”, Chem. Eur. J., 2008, vol. 14, pp. 4939-4947.
[cited by applicant]
Sheen et al., “Stimulating Antitumor Immunity with Nanoparticles”, Wiley Interdiscip Rev Nanomed Nanobiotechnol, Oct. 2014, vol. 6, pp. 496-505.
[cited by applicant]
Office action for Chinese Patent Application No. 201580063662.6, dated Mar. 4, 2020.
[cited by applicant]
Office action for European Patent Application No. 15 857 504.3-1111, dated Mar. 18, 2020.
[cited by applicant]
Yildiz et al., “Applications of viral nanoparticles in medicine”, Current Opinion in Biotechnology, vol. 22, Issue 6, pp. 901-908.
[cited by applicant]
Aljabali, et al., “CPMV-DOX Delivers”, Molecular Pharmaceutics, 2013, 10, pp. 3-10.
[cited by applicant]
Wen, et al., “Interior Engineering of a Viral Nanoparticle and its Tumor Homing Properties” Macromolecules, vol. 13, No. 12, Dec. 2012.
[cited by applicant]
Agrawal, et al., “Differential Uptake of Chemically Modified Cowpea Mosaic Virus Nanoparticles in Macrophage Subpopulations Present in Inflammatory and Tumor Microenvironments”, Biomacromolecules, vol. 13, No. 10, Oct. …
[cited by applicant]
Brennan, et al., “Cowpea Mosaic Virus as a Vaccine Carrier of Heterologous Antigens”, Molecular Biotechnology, vol. 17, No. 1, Jan. 2001.
[cited by applicant]
Gonzalez, et al., “Interaction of Cowpea Mosaic Virus (CPMV) Nanoparticles with Antigen Presenting Cells in Vitro and In Vivo”, Plos One, vol. 4, No. 11, Nov. 2009.
[cited by applicant]
Izotte, et al., “Plant-derived viral-like nanoparticle immunotherapy suppress development of metastatic lung cancer”, Journal of Immunology, vol. 194, Issue 1 Supplement, May 2015.
[cited by applicant]
Patrick H. Lizotte, “Novel approaches to targeting innate immunity for cancer immunotherapy”, Proquest Dissertations Publishing, May 2015.
[cited by applicant]
Supplementary European Search Report for Patent Application No. 15857504.3-1111/3215520, dated May 28, 2018.
[cited by applicant]
International Search Report for Application No. PCT/US15/59675.
[cited by applicant]
Smyth et al. Treatment of rapidly growing K-BALB and CT26 mouse tumours using Semliki Forest virus and its derived vector. Gene Therapy (2005) 12, 147-159.
[cited by applicant]
Inventor: Nicole Steinmetz, “Rod-Shaped Plant Virus Nanoparticles as Imaging Agent Platforms”; U.S. Appl. No. 16/149,828, filed Oct. 2, 2018, Office Action dated Aug. 28, 2020, 22 pgs.
[cited by applicant]
Applicant: Case Western Reserve University; “Cancer Immunotherapy Using Virus Particles”; Office Action, dated Aug. 4, 2020; 3 pgs.
[cited by applicant]
Applicant: Case Western Reserve University; “Plant Virus Particles for Delivery of Antimitotic Agents”; Extended European Search Report; dated Aug. 17, 2020; 11 pgs.
[cited by applicant]
Canan Uluog, et al.: “Intermediate dose of methotrexate toxicity in non-Hodgkin lymphoma”, General Pharmacology, vol. 32, 1999, pp. 215-218, XP55711259.
[cited by applicant]
Trevor W. E. Robinson, et al., “The Journal of Investigative Dermatology the Effect of Methotrexate on Cell Division in the Epidermis of the Young Rat”; The Journal of investigative Dermatology, vol. 53, 1969, pp. 223-2…
[cited by applicant]
Jantipa Jobsri, et al.: Plant Virus Particles Carrying Tumour Antigen Activate TLR7 and Induce High Levels of Protective Antibody, Plos One, vol. 10, No. 2, Jan. 1, 2015, pp. 1-16, XP055347065, DOI: 10.1371/journal.pone…
[cited by applicant]
Pfizer Ltd.: “Package leaflet: Information for the patient”, Jan. 1, 2014, XP55565400, Walton Oaks, Tadworth, Surrey, UK Retrieved from the Internet: URL:https://www.medicines.org.uk/emc/files/pil.6184.pdf [retrieved on…
[cited by applicant]
Alaa A. Al. Aljabali, et al.; “CPMV-DOX Delivers”, Molecular Pharmaceutics, vol. 10, No. 1, Jan. 7, 2013, pp. 3-10, XP055347068, US ISSN: 1543-8384, DOI: 10.1021/MP3002057.
[cited by applicant]
Sourabh Shukla, et al.: “The Impact of Aspect Ratio on the Biodistribution and Tumor Homing of Rigid Soft-Matter Nanorods”, Advanced Healthcare Materials, vol. 4, No. 6, Apr. 1, 2015, pp. 874-882, XP055473103, DE ISSN: …
[cited by applicant]
Francisco, Joseph A., et al.; “cAC10-vcMMAE, an anti-CD30-monomethyl auristatin E conjugate with potent and selective antitumor activity”, Blood, American Society of Hematology, US, vol. 102, No. 4, Aug. 15, 2003, pp. 1…
[cited by applicant]
Shivprasad et al., “Hererologous Sequences Greatly Affect Foreign Gene Expression in Tobacco Mosaic Virus-Based Vectors,” Virology, Mar. 15, 1999 (Mar. 15, 1999). vol. 255, pp. 312-323.
[cited by applicant]
Chariou, et al., “Delivery of Pesticides to Plant Parasitic Nematodes Using Tobacco Mild Green Mosaic Virus as a Nanocarrier,” ACS Nano, May 23, 2017 (May 23, 2017), vol. 11, No. 5 pp. 4719-4730.
[cited by applicant]
Czapar, et al., “Tobacco Mosaic Virus Delivery of Phenanthriplatin for Cancer Therapy,” ACS Nano, Mar. 28, 2016 (Mar. 28, 2016), vol. 10, No. 4, pp. 4119-4126.
[cited by applicant]
Lee, et al., “Genetic Engineering and Chemical Conjugation of Potato Virus X,” Methods in Molecular Biology, Chapter: Virus Hybrids as Nanomaterials, Oct. 30, 2013 (Oct. 30, 2013), vol. 1108, pp. 3-21.
[cited by applicant]
Applicant: Case Western Reserve University; “Cancer Immunotherapy Using Virus Particles”; European Patent Application No. 18764856.3 for Supplementary European Search Report dated Dec. 22, 2020; 8 pgs.
[cited by applicant]
Lee, K. L., et al.; “Combination of Plant Virus Nanoparticle-Based in Situ Vaccination with Chemotherapy Potentiates Antitumor Response”. Nano letters, 17(7); Epub Jun. 26, 2017; 4019-4028. https://doi.org/10.1021/acs.n…
[cited by applicant]
Nicole F.Steinmetz, et al.; “Coated Plant Virus Imaging Agents”; U.S. Appl. No. 16/279,482, filed Feb. 19, 2019; Non-Final Rejection dated Mar. 23, 2021; 91 pgs.
[cited by applicant]
Nicole F.Steinmetz; “Viral Nanoparticle Multimers”; U.S. Appl. No. 14/761,444, filed Jul. 16, 2015; Final Office Action dated Mar. 11, 2021; 11 pgs.
[cited by applicant]
Czapar, Anna et al. Tobacco Mosaic Virus Delivery of Phenanthriplatin for Cancer therapy. American Chemical Society. Nano 2016 (10) pp. 4119 4126 (Year: 2016).
[cited by applicant]
Le, Duc et al. Biodistribution of Filamentous Plant Virus Nanoparticles: Pepino Mosaic Virus versus Potato Virus X. Biomacromolecules 219 Jan. 14; 20(a): pp. 469-477. (Year 2019).
[cited by applicant]
Le, Duc et al. Chemical addressability of potoato virus X for its applications in bio/nanotechnology. El Sevier. Journal of Structural Biology 200 (2017). pp. 360-368. (Year: 2017).
[cited by applicant]
Le, Duc et al. Potato virus X, a filamentous plant viral nanoparticle for doxorubicin delivery in cancer therapy. Royal Society of Chemistry. Nanoscale, 2017 (9). pp. 2348-2357. (Year 2017).
[cited by applicant]
Nicole F. Steinmetz, U.S. Appl. No. 16/998,210, filed Aug. 7, 2020; Non-Final OA dated Dec. 7, 2022.
[cited by applicant]
Tran, Hong Hanh. Developing a plant virus-based expression system for the expression of vaccines against Porcine Reproductive and Respiratory Syndrome Virus. Western Graduate & Postdoctoral Studies. Electronic Thesis an…
[cited by applicant]
Bruckman et al. (Nano Letters. Mar. 2014; 14: 1551-1558).
[cited by applicant]
Mamura et al. (“FOXA 1 promotes tumor progression in prostate cancer via the insulin-like growth factor binding protein 3 pathway.” (2012).
[cited by applicant]
Lam, et al. (WIREs Nanomed Nanobiotechnol Jan./Feb. 2018 vol. 10: 1-18).
[cited by applicant]
Mitoxantrone. Drug Bank Online. Website. https://go.drugbank.com/drugs/DB01204. (Accessed Dec. 15, 2022) (Year: 2022).
[cited by applicant]
Mosquera et al. (Acc. Chem. Res. 2018, 51, 9, 2305-2313 Publication Date: Aug. 29, 2018.
[cited by applicant]
Nicole F.Steinmetz; U.S. Appl. No. 16/597,509, filed Oct. 9, 2019; Non-Final Office Action, dated Dec. 27, 2022; 12 pgs.
[cited by applicant]
Nicole F.Steinmetz; U.S. Appl. No. 16/759,652, filed Apr. 27, 2020; Final Office Action, dated Dec. 12, 2022; 15 pgs.
[cited by applicant]
Nicole F.Steinmetz; U.S. Appl. No. 17/129,463, filed Dec. 21, 2020; Non-Final Office Action, dated Dec. 8, 2022; 32 pgs.
[cited by applicant]
Nicole F.Steinmetz;U.S. Appl. No. 17/522,182, filed Nov. 9, 2021; Non-Final Office Action, dated Jan. 5, 2023; 27 pgs.
[cited by applicant]
Nicole F.Steinmetz; U.S. Appl. No. 17/677,147, filed Feb. 22, 2022; Non-Final Office Action, dated Jan. 13, 2023; 22 pgs.
[cited by applicant]
Pellico et al. (Contrast Media and Molecular Imaging. 2019; Article ID 1845637: 1-13).
[cited by applicant]
Pretto et al. (“Versatile reversible cross-linking strategy to stabilize CCMV virus like particles for efficient siRNA delivery.” Bioconjugate chemistry 30.12 (2019): 3069-3077).
[cited by applicant]
Royston et al. (Journal of Colloidal and Interface Science. 2009; 332: 402-407).
[cited by applicant]
Tamoxifen. Drug Bank Online. Website. https://go.drugbank.com/drugs/DB00675. (Accessed: Dec. 15, 2022) (Year: 2022).
[cited by applicant]
Temming et al. (bioconjugate Chemistry. 2006; 17: 1385-1394).
[cited by applicant]
Kiao et al. (International Journal of Molecular Medicine. 2016; 38: 1319-326).
[cited by applicant]
Zhang et al. (Theranostics. 2018; 8 (9): 2521-2548).
[cited by applicant]
Agrawal Arpita et al: “Differential Uptake of Chemically Modified Cowpea Mosaic Virus Nanoparticles in Macrophage Subpopulations Present in Inflammatory and Tumor Microenvironments”, Biomacromolecules, vol. 13, No. 10, …
[cited by applicant]
Applicant: Case Western Reserve University; “Cancer Immunotherapy Using Virus Particles”; European Patent Application No. 21201960.8; Extended European Search Report dated Jan. 19, 2022; 11 pgs.
[cited by applicant]
Brennan Frank R et al: “Cowpea mosaic virus as a vaccine carrier of heterologous antigens”, Molecular Biotechnology, vol. 17, No. 1, Jan. 2001 (Jan. 2001), pp. 15-26, XP002780312, ISSN: 1073-6085.
[cited by applicant]
Gonzalez Maria Jet Al: “Interaction of Cowpea Mosaic Virus (CPMV) Nanoparticles with Antigen Presenting Cells In Vitro and In Vivo”, Plos One, vol. 4, No. 11, Nov. 2009 (Nov. 2009), KP002780311, ISSN: 1932-6203.
[cited by applicant]
Patrick h. Iizotte: “Novel approaches to targeting innate immunity for cancer Immunotherapy”, Proquest Dissertations Publishing, May 2015 (May 2015), XP002780316, Retrieved from the Internet: URL:https://search.proquest…
[cited by applicant]
Saunders Ket Al: “Efficient generation of cowpea mosaicvirus empty virus-like particles by the proteolytic processing of precursors in insect cells and plants”, Virology, Elsevier, Amsterdam, NL, vol. 393, No. 2, Oct. 2…
[cited by applicant]