IP Library › Granted Patent US 12,600,817
Granted Patent B2
US 12,600,817 · App. 19/052,121 · Granted Apr 14, 2026

Devices and methods for analyzing biological samples

Inventors: Seyedsina Moeinzadeh (Mountain View, CA); Justin Poelma (Sunnyvale, CA); Meng Taing (Hayward, CA); Frank Charbonier (San Francisco, CA); Tarun Kumar Khurana (Fremont, CA)
Assignee: Cellanome, Inc.
C08G65/3348C08G65/2609C08G65/33396C08J3/24C08J3/28C08J2371/02
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Quick Facts
Patent No.
US 12,600,817
App. No.
19/052,121
Granted
Apr 14, 2026
Kind
B2
Abstract

Systems for analyzing biological components are provided. The systems may include a fluidic device and an energy source in communication with the fluidic device. The energy source may supply energy to the fluidic device to form a polymer matrix on or adjacent to a biological component within the fluidic device. Methods of using the systems to analyze biological components are also provided.

Claims (101)

1 . A polymer precursor, comprising:

an oligomeric domain comprising three or more arms, wherein each arm of the oligomeric domain comprises a degradable functional group and a crosslinkable functional group,

wherein the crosslinkable functional group of an arm of the three or more arms is configured to crosslink in response to a first stimulus, thereby obtaining a polymerized form of the polymer precursor, and

wherein the degradable functional group is configured to be cleaved in response to a second stimulus, thereby solubilizing the polymerized form of the polymer precursor, wherein the second stimulus comprises introduction of a degradation reagent, and wherein the degradation reagent comprises dithiothreitol (DTT), tris (2-carboxyethyl) phosphine (TCEP), 2-mercaptoethanol (BME), glutathione reductase (GSH), sodium (meta) periodate, alginate lyase, dextranase, lysozyme, chitinase, hyaluronidase, chondroitinase, cellulases, or a combination thereof.

2 . The polymer precursor of claim 1 , wherein the oligomeric domain comprises four or more arms.

3 . The polymer precursor of claim 1 , wherein the oligomeric domain consists of four arms.

4 . The polymer precursor of claim 1 , wherein the oligomeric domain is hydrophilic.

5 . The polymer precursor of claim 1 , wherein the oligomeric domain comprises poly(ethylene glycol), poly(N-vinylpyrrolidone), poly(acrylic acid), poly(methacrylic acid), poly(vinyl alcohol), poly(L-lysine), poly(2-ethyl-2-oxazoline), poly(maleic acid), poly(vinyl phosphoric acid), poly(acrylamide), poly(vinylamine), poly(ethylene oxide-co-propylene oxide), poly(N-isopropylacrylamide), poly(vinyl phosphonic acid), poly(2-vinyl-1-methylpyridinium bromide), poly(N,N-diethylacrylamide), poly(N,N-dimethylacrylamide), poly(2-hydroxyethyl acrylate), poly(2-hydroxy methacrylate-co-methacrylic acid), poly(propylene oxide), poly(2-(dimethylamino) ethyl methacrylate), or poly(N-(2-hydroxypropyl) methacrylamide), or a combination thereof.

6 . The polymer precursor of claim 5 , wherein the oligomeric domain comprises poly(ethylene glycol).

7 . The polymer precursor of claim 1 , wherein the degradable functional group comprises disulfide, vicinal diol, polysaccharides, or a combination thereof.

8 . The polymer precursor of claim 1 , wherein the degradable functional group comprises disulfide.

9 . The polymer precursor of claim 1 , wherein the degradable functional group comprises vicinal diol.

10 . The polymer precursor of claim 1 , wherein the degradable functional group is enzymatically degradable.

11 . The polymer precursor of claim 1 , wherein the crosslinkable functional group comprises acrylate, methacrylate, acrylamide, methacrylamide, norbornyl, styrene, vinyl ether, vinyl pyrrolidone, vinyl ester, maleimide, allyl, alkyne, azide, thiol, alkene, or epoxide, or a combination thereof.

12 . The polymer precursor of claim 1 , wherein the oligomeric domain comprises polyethylene glycol, wherein the degradable functional group comprises disulfide, and wherein the crosslinkable functional group comprises acrylamide.

13 . The polymer precursor of claim 12 , wherein the oligomeric domain further comprises thioether.

14 . The polymer precursor of claim 1 , wherein the oligomeric domain comprises polyethylene glycol, wherein the degradable functional group comprises vicinal diol, and wherein the crosslinkable functional group comprises acrylamide.

15 . The polymer precursor of claim 14 , wherein the oligomeric domain further comprises thioether.

16 . The polymer precursor of claim 1 , wherein the first stimulus comprises light.

17 . The polymer precursor of claim 16 , wherein the light is at a wavelength from about 300 to about 500 nm.

18 . The polymer precursor of claim 1 , wherein the oligomeric domain further comprises an amide moiety, a beta-thioether moiety, or a combination thereof.

19 . A kit, comprising: (a) a porogen, wherein the porogen comprises poly(ethylene glycol); and (b) a polymer precursor comprising:

an oligomeric domain comprising three or more arms, wherein each arm of the oligomeric domain comprises a degradable functional group and a crosslinkable functional group,

wherein the crosslinkable functional group of an arm of the three or more arms is configured to crosslink in response to a first stimulus, thereby obtaining a polymerized form of the polymer precursor, and

wherein the degradable functional group is configured to be cleaved in response to a second stimulus, thereby solubilizing the polymerized form of the polymer precursor.

20 . The kit of claim 19 , wherein the ratio of the porogen to the polymer precursor by weight is from about 1:1 to about 1:6.

21 . The kit of claim 19 , wherein (a) the porogen and (b) the polymer precursor are contained in a single container.

22 . The kit of claim 21 , further comprising (c) a photoinitiator contained in another container.

23 . The kit of claim 22 , wherein the photoinitiator includes lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP), Irgacure 2959, diphenyl(2,4,6-trimethylbenzoyl)phosphine oxide (TPO) nanoparticles, 2,2′-azobis[2-methyl-N-(2-hydroxyethyl)promionamide] (VA-086), BAPO-Oli, BAPO-Ona, eosin-Y, riboflavin, or combinations thereof.

24 . The kit of claim 22 , wherein the photoinitiator comprises lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP).

25 . The kit of claim 19 , wherein a free thiol concentration of the polymer precursor is less than 5%.

26 . A polymer precursor, comprising:

an oligomeric domain comprising three or more arms, wherein each arm of the oligomeric domain comprises a degradable functional group and a crosslinkable functional group,

wherein the crosslinkable functional group of an arm of the three or more arms is configured to crosslink in response to a first stimulus, thereby obtaining a polymerized form of the polymer precursor, and

wherein the degradable functional group is configured to be cleaved in response to a second stimulus, thereby solubilizing the polymerized form of the polymer precursor, and wherein the second stimulus comprises introduction of an oxidizing agent.

27 . The polymer precursor of claim 26 , wherein the oligomeric domain comprises four or more arms.

28 . The polymer precursor of claim 26 , wherein the oligomeric domain consists of four arms.

29 . The polymer precursor of claim 26 , wherein the oligomeric domain is hydrophilic.

30 . The polymer precursor of claim 26 , wherein the oligomeric domain comprises poly(ethylene glycol), poly(N-vinylpyrrolidone), poly(acrylic acid), poly(methacrylic acid), poly(vinyl alcohol), poly(L-lysine), poly(2-ethyl-2-oxazoline), poly(maleic acid), poly(vinyl phosphoric acid), poly(acrylamide), poly(vinylamine), poly(ethylene oxide-co-propylene oxide), poly(N-isopropylacrylamide), poly(vinyl phosphonic acid), poly(2-vinyl-1-methylpyridinium bromide), poly(N,N-diethylacrylamide), poly(N,N-dimethylacrylamide), poly(2-hydroxyethyl acrylate), poly(2-hydroxy methacrylate-co-methacrylic acid), poly(propylene oxide), poly(2-(dimethylamino) ethyl methacrylate), or poly(N-(2-hydroxypropyl) methacrylamide), or a combination thereof.

31 . The polymer precursor of claim 30 , wherein the oligomeric domain comprises poly(ethylene glycol).

32 . The polymer precursor of claim 26 , wherein the degradable functional group comprises vicinal diol.

33 . The polymer precursor of claim 26 , wherein the crosslinkable functional group comprises acrylate, methacrylate, acrylamide, methacrylamide, norbornyl, styrene, vinyl ether, vinyl pyrrolidone, vinyl ester, maleimide, allyl, alkyne, azide, thiol, alkene, or epoxide, or a combination thereof.

34 . The polymer precursor of claim 26 , wherein the oligomeric domain comprises polyethylene glycol, wherein the degradable functional group comprises vicinal diol, and wherein the crosslinkable functional group comprises acrylamide.

35 . The polymer precursor of claim 34 , wherein the oligomeric domain further comprises thioether.

36 . The polymer precursor of claim 26 , wherein the first stimulus comprises light.

37 . The polymer precursor of claim 36 , wherein the light is at a wavelength from about 300 to about 500 nm.

38 . The polymer precursor of claim 26 , wherein the oligomeric domain further comprises an amide moiety, a beta-thioether moiety, or a combination thereof.

39 . A polymer precursor, comprising:

an oligomeric domain comprising three or more arms, wherein each arm of the oligomeric domain comprises a degradable functional group and a crosslinkable functional group,

wherein the crosslinkable functional group of an arm of the three or more arms is configured to crosslink in response to a first stimulus, thereby obtaining a polymerized form of the polymer precursor, and

wherein the degradable functional group is configured to be cleaved in response to a second stimulus, thereby solubilizing the polymerized form of the polymer precursor, wherein the second stimulus comprises introduction of a reducing agent.

40 . The polymer precursor of claim 39 , wherein the oligomeric domain comprises four or more arms.

41 . The polymer precursor of claim 39 , wherein the oligomeric domain consists of four arms.

42 . The polymer precursor of claim 39 , wherein the oligomeric domain is hydrophilic.

43 . The polymer precursor of claim 39 , wherein the oligomeric domain comprises poly(ethylene glycol), poly(N-vinylpyrrolidone), poly(acrylic acid), poly(methacrylic acid), poly(vinyl alcohol), poly(L-lysine), poly(2-ethyl-2-oxazoline), poly(maleic acid), poly(vinyl phosphoric acid), poly(acrylamide), poly(vinylamine), poly(ethylene oxide-co-propylene oxide), poly(N-isopropylacrylamide), poly(vinyl phosphonic acid), poly(2-vinyl-1-methylpyridinium bromide), poly(N,N-diethylacrylamide), poly(N,N-dimethylacrylamide), poly(2-hydroxyethyl acrylate), poly(2-hydroxy methacrylate-co-methacrylic acid), poly(propylene oxide), poly(2-(dimethylamino) ethyl methacrylate), or poly(N-(2-hydroxypropyl) methacrylamide), or a combination thereof.

44 . The polymer precursor of claim 43 , wherein the oligomeric domain comprises poly(ethylene glycol).

45 . The polymer precursor of claim 39 , wherein the degradable functional group comprises disulfide.

46 . The polymer precursor of claim 39 , wherein the crosslinkable functional group comprises acrylate, methacrylate, acrylamide, methacrylamide, norbornyl, styrene, vinyl ether, vinyl pyrrolidone, vinyl ester, maleimide, allyl, alkyne, azide, thiol, alkene, or epoxide, or a combination thereof.

47 . The polymer precursor of claim 39 , wherein the oligomeric domain comprises polyethylene glycol, wherein the degradable functional group comprises disulfide, and wherein the crosslinkable functional group comprises acrylamide.

48 . The polymer precursor of claim 47 , wherein the oligomeric domain further comprises thioether.

49 . The polymer precursor of claim 39 , wherein the first stimulus comprises light.

50 . The polymer precursor of claim 49 , wherein the light is at a wavelength from about 300 to about 500 nm.

51 . The polymer precursor of claim 39 , wherein the oligomeric domain further comprises an amide moiety, a beta-thioether moiety, or a combination thereof.

52 . A polymer precursor, comprising:

an oligomeric domain comprising three or more arms, wherein each arm of the oligomeric domain comprises a degradable functional group and a crosslinkable functional group,

wherein the crosslinkable functional group of an arm of the three or more arms is configured to crosslink in response to a first stimulus, thereby obtaining a polymerized form of the polymer precursor, and

wherein the degradable functional group is configured to be cleaved in response to a second stimulus, thereby solubilizing the polymerized form of the polymer precursor, and wherein the oligomeric domain further comprises an amide moiety, a beta-thioether moiety, or a combination thereof.

53 . The polymer precursor of claim 52 , wherein the oligomeric domain comprises four or more arms.

54 . The polymer precursor of claim 52 , wherein the oligomeric domain consists of four arms.

55 . The polymer precursor of claim 52 , wherein the oligomeric domain is hydrophilic.

56 . The polymer precursor of claim 52 , wherein the oligomeric domain comprises poly(ethylene glycol), poly(N-vinylpyrrolidone), poly(acrylic acid), poly(methacrylic acid), poly(vinyl alcohol), poly(L-lysine), poly(2-ethyl-2-oxazoline), poly(maleic acid), poly(vinyl phosphoric acid), poly(acrylamide), poly(vinylamine), poly(ethylene oxide-co-propylene oxide), poly(N-isopropylacrylamide), poly(vinyl phosphonic acid), poly(2-vinyl-1-methylpyridinium bromide), poly(N,N-diethylacrylamide), poly(N,N-dimethylacrylamide), poly(2-hydroxyethyl acrylate), poly(2-hydroxy methacrylate-co-methacrylic acid), poly(propylene oxide), poly(2-(dimethylamino) ethyl methacrylate), or poly(N-(2-hydroxypropyl) methacrylamide), or a combination thereof.

57 . The polymer precursor of claim 56 , wherein the oligomeric domain comprises poly(ethylene glycol).

58 . The polymer precursor of claim 52 , wherein the degradable functional group comprises disulfide, vicinal diol, beta-thioether ester, amidomethylol, peptide, polysaccharides (e.g. alginate, dextran, chitosan, chondroitin), ortho-nitrobenzyl, coumarin, acetal, ketal, ester, ortho ester, anhydride, imine, hydrazone, carbonate, phosphate, cinnamoyl, or benzoin ether, or a combination thereof.

59 . The polymer precursor of claim 58 , wherein the degradable functional group comprises disulfide.

60 . The polymer precursor of claim 58 , wherein the degradable functional group comprises vicinal diol.

61 . The polymer precursor of claim 52 , wherein the degradable functional group is enzymatically degradable.

62 . The polymer precursor of claim 52 , wherein the crosslinkable functional group comprises acrylate, methacrylate, acrylamide, methacrylamide, norbornyl, styrene, vinyl ether, vinyl pyrrolidone, vinyl ester, maleimide, allyl, alkyne, azide, thiol, alkene, or epoxide, or a combination thereof.

63 . The polymer precursor of claim 52 , wherein the oligomeric domain comprises polyethylene glycol, wherein the degradable functional group comprises disulfide, and wherein the crosslinkable functional group comprises acrylamide.

64 . The polymer precursor of claim 63 , wherein the oligomeric domain comprises thioether.

65 . The polymer precursor of claim 52 , wherein the oligomeric domain comprises polyethylene glycol, wherein the degradable functional group comprises vicinal diol, and wherein the crosslinkable functional group comprises acrylamide.

66 . The polymer precursor of claim 65 , wherein the oligomeric domain comprises thioether.

67 . The polymer precursor of claim 52 , wherein the first stimulus comprises light.

68 . The polymer precursor of claim 67 , wherein the light is at a wavelength from about 300 to about 500 nm.

69 . The polymer precursor of claim 52 , wherein the second stimulus comprises an energy source in a presence of a photoinitiator and an absence of polymer precursor.

70 . The polymer precursor of claim 52 , wherein the second stimulus comprises introduction of a degradation reagent, and wherein the degradation reagent comprises dithiothreitol (DTT), tris (2-carboxyethyl) phosphine (TCEP), 2-mercaptoethanol (BME), glutathione reductase (GSH), sodium (meta) periodate, alginate lyase, dextranase, lysozyme, chitinase, hyaluronidase, chondroitinase, cellulases, or a combination thereof.

71 . The polymer precursor of claim 52 , wherein the second stimulus comprises introduction of an oxidizing agent.

72 . The polymer precursor of claim 52 , wherein the second stimulus comprises introduction of a reducing agent.

73 . A polymer precursor, comprising:

an oligomeric domain comprising three or more arms, wherein each arm of the oligomeric domain comprises a degradable functional group and a crosslinkable functional group,

wherein the crosslinkable functional group of an arm of the three or more arms is configured to crosslink in response to a first stimulus, thereby obtaining a polymerized form of the polymer precursor, and

wherein the degradable functional group is configured to be cleaved in response to a second stimulus, thereby solubilizing the polymerized form of the polymer precursor, and wherein the degradable functional group comprises disulfide.

74 . The polymer precursor of claim 73 , wherein the oligomeric domain consists of four arms.

75 . The polymer precursor of claim 73 , wherein the crosslinkable functional group comprises acrylate, methacrylate, acrylamide, methacrylamide, norbornyl, styrene, vinyl ether, vinyl pyrrolidone, vinyl ester, maleimide, allyl, alkyne, azide, thiol, alkene, or epoxide, or a combination thereof.

76 . The polymer precursor of claim 73 , wherein the crosslinkable functional group comprises alkene.

77 . A polymer precursor, comprising:

an oligomeric domain comprising three or more arms, wherein each arm of the oligomeric domain comprises a degradable functional group and a crosslinkable functional group,

wherein the crosslinkable functional group of an arm of the three or more arms is configured to crosslink in response to a first stimulus, thereby obtaining a polymerized form of the polymer precursor, and

wherein the degradable functional group is configured to be cleaved in response to a second stimulus, thereby solubilizing the polymerized form of the polymer precursor, and wherein the degradable functional group comprises vicinal diol.

78 . The polymer precursor of claim 77 , wherein the oligomeric domain consists of four arms.

79 . The polymer precursor of claim 77 , wherein the crosslinkable functional group comprises acrylate, methacrylate, acrylamide, methacrylamide, norbornyl, styrene, vinyl ether, vinyl pyrrolidone, vinyl ester, maleimide, allyl, alkyne, azide, thiol, alkene, or epoxide, or a combination thereof.

80 . The polymer precursor of claim 77 , wherein the crosslinkable functional group comprises alkene.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Mar 3, 2025
From: MOEINZADEH, SEYEDSINA; POELMA, JUSTIN; TAING, MENG; CHARBONIER, FRANK; KHURANA, TARUN KUMAR
To: CELLANOME, INC.
Reel/Frame 070382/0032 →
Continuity (3)
Continuation PCTUS2024059913 · Dec 12, 2024
Provisional Application 63609847 · Dec 13, 2023
Related Publication 20250282912A1 · Sep 11, 2025
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