IP Library › Granted Patent US 12,600,976
Granted Patent B2
US 12,600,976 · App. 18/626,739 · Granted Apr 14, 2026

Maize gene KRN2 and uses thereof

Inventors: Xiaohong Yang (Beijing, CN); Jiansheng Li (Beijing, CN); Wenkang Chen (Beijing, CN); Xuan Zhang (Beijing, CN); Lichun Cai (Beijing, CN); Yirong Zhang (Beijing, CN)
Assignee: CHINA AGRICULTURAL UNIVERSITY
C12N15/8218C12N15/8261C07K14/415
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Quick Facts
Patent No.
US 12,600,976
App. No.
18/626,739
Granted
Apr 14, 2026
Kind
B2
Abstract

Provided herein are KRN2 gene controlling kernel row number in plant, molecular markers closely linked to KRN2 and their application in molecular breeding.

Claims (6)

1 . A construct comprising a sequence encoding a guide RNA designed to target the KRN2 gene, wherein the expression of the construct in a maize plant together with the expression of a Cas-associated gene results in a loss-of-function in the KRN2 gene.

2 . A transgenic maize plant, maize plant part or maize plant cell thereof comprising the construct of claim 1 .

3 . A seed produced by growing the transgenic maize plant according to claim 2 , wherein the seed comprises the loss-of-function in the KRN2 gene.

4 . A maize plant, maize plant cell, or maize plant parts thereof, grown from the seed of claim 3 .

5 . A commodity product made from the maize plant, maize plant cell, or maize plant parts thereof, of claim 4 , wherein the commodity product comprises a construct comprising a sequence encoding a guide RNA designed to target the KRN 2 gene, wherein the expression of the construct in a maize plant together with the expression of a Cas-associated gene results in a loss-of-function in the KRN2 gene.

6 . The commodity product of claim 5 , wherein the commodity product is protein concentrate, protein isolate, cereal, meal, flour or biomass.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 4, 2024
From: YANG, XIAOHONG; LI, JIANSHENG; CHEN, WENKANG; ZHANG, XUAN; CAI, LICHUN; ZHANG, YIRONG
To: CHINA AGRICULTURAL UNIVERSITY
Reel/Frame 067008/0859 →
Priority Claims (1)
CN 201711217216.3 · Nov 28, 2017 · national
Continuity (2)
Continuation 16767207
Related Publication 20240376487A1 · Nov 14, 2024
References Cited (28)
US 11976285B2 · Yang · 2024 [cited by examiner]
US 20060141495A1 · Wu · 2006 [cited by applicant]
US 20100313299A1 · Sanz Molinero et al. · 2010 [cited by applicant]
US 20170114356A1 · Li et al. · 2017 [cited by applicant]
CN 101883783A · 2010 [cited by applicant]
CN 103687950A · 2014 [cited by applicant]
CN 103951741A · 2014 [cited by applicant]
CN 107354215A · 2017 [cited by applicant]
WO 2009068564A1 · 2009 [cited by applicant]
WO 2009091518A2 · 2009 [cited by applicant]
WO 2014106838A2 · 2014 [cited by applicant]
Chen et al (Convergent selection of a WD40 protein that enhances grain yield in maize and rice. Science. p. 1-9, 2022) (Year: 2022). [cited by examiner]
Ali et al (Seed Composition and Seed Oil Antioxidant Activity of Maize Under Water Stress. J Am Oil Chem Soc. 87:1179-1187, 2010) (Year: 2010). [cited by examiner]
Ali et al., Seed Composition and Seed Oil Antioxidant Activity of Maize Under Water Stress. J Am Oil Chem Soc. 2010;87:1-9. [cited by applicant]
Ge et al., Minimal-length short hairpin RNAs: the relationship of structure and RNAi activity. RNA. Jan. 2010;16 (1):106-17. [cited by applicant]
GenBank Accesson No. ONM14875, Transducin/WD40 repeat-like superfamily protein [ [cited by applicant]
Hall, Unravelling the general properties of siRNAs: strength in Nos. and lessons from the past. Nat Rev Genet. Jul. 2004;5(7):552-7. [cited by applicant]
Li et al., Combined Linkage and Association Mapping Reveals QTL and Candidate Genes for Plant and Ear Height in Maize. Front Plant Sci. Jun. 15, 2016;7:833, 11 page. [cited by applicant]
Muhammad et al., RNA Interference: A Natural Immune System of Plants to Counteract Biotin Stressors. Cells. Jan. 10, 2019;8(1):38, 29 pages. [cited by applicant]
NCBI Reference No. XP_008669015.1. WD repeat-containing protein 44 [ [cited by applicant]
Sekhon et al., Genome-wide atlas of transcription during maize development. Plant J. 2011;66(4):553-563. [cited by applicant]
STN Accession No. OEL26113, 2 pages, Sep. 28, 2016. [cited by applicant]
Su et al., High Density Linkage Map Construction and Mapping of Yield Trait QTLs in Maize ( [cited by applicant]
Sun, Bioinformatics Analysis of the Gene GRMZM2G398848 Related to Kernal Row No. in Maize. Heilongjiang Agricultural Sciences. Dec. 31, 2016;11:4-7. [cited by applicant]
UniProtKB/TrEMBL Accession No. A0A1D6E2N4, 8 pages, (2016). [cited by applicant]
Wang, Combining Large Recombinant Inbred Lines Population and Ultra-high Density Molecular Markers to Identify QTL for Important Agronomic Traits in Maize. Chinese Doctoral Dissertation Full Text Database, Thesis. 93 pa… [cited by applicant]
International Search Report for Application No. PCT/CN2018/117844, dated Feb. 27, 2019, 5 pages. [cited by applicant]
U.S. Appl. No. 16/767,207, filed May 27, 2020, U.S. Pat. No. 11,976,285, Issued. [cited by applicant]