Method for producing t cells modified by chimeric antigen receptor
A method for producing γδ T cells modified by a chimeric antigen receptor, comprising: transfecting K562 cells with shFPPS targeted to FPP synthase by means of a lentiviral vector, such that the expression of FPPS in the K562 cells is lowered and a K562-shFPPS cell line with reduced FPPS expression is constructed; adding the K562-shFPPS cell line into a γδ T cell culture system for co-culturing with the γδ T cells, wherein it is found that the K562-shFPPS cell line can facilitate in vitro differentiation and proliferation of the γδ T cells; and adding a CAR-expressing lentiviral vector to the γδ T cell culture system comprising the cell line for co-culturing, wherein it is found that the K562-shFPPS cell line can further effectively improve the transfection rate of CAR genes. The provided solution effectively overcomes the technical challenge of the large-scale production of CAR-γδ T cells, and has a good application prospect.
1 . A method for expanding γδ T cells, comprising:
introducing shRNA that inhibits the expression of FPPS (Farnesyl pyrophosphate Synthase)-encoding gene into tumor cells to obtain tumor cells with reduced FPPS expression and activity;
adding the tumor cells with reduced FPPS expression and activity to a γδ T cell culture system,
wherein the shRNA that inhibits the expression of FPPS-encoding gene is single-stranded RNA comprising in 5′ to 3′ order: a stem I, a loop and a stem II;
wherein the stem I consists of the sequence of positions 1-21 of SEQ ID NO: 2; the loop consists of the sequence of positions 22-27 of SEQ ID NO: 2; and the stem II consists of the sequence of positions 28-48 of SEQ ID NO: 2.
2 . The method according to claim 1 , wherein a ratio of the number of the γδ T cells to the number of the tumor cells with reduced FPPS expression and activity is (1-10):1.
3 . A method for producing CAR-γδ T cells, comprising:
a) introducing shRNA that inhibits the expression of FPPS (Farnesyl pyrophosphate Synthase)-encoding gene into tumor cells to obtain tumor cells with reduced FPPS expression and activity; adding the tumor cells with reduced FPPS expression and activity to a γδ T cell culture system and culturing to obtain a mixed culture system; and
b) adding a vector encoding a chimeric antigen receptor (CAR) to the mixed culture system and culturing to obtain CAR-γδ T cells,
wherein the shRNA that inhibits the expression of FPPS-encoding gene is single-stranded RNA comprising in 5′ to 3′ order a stem I, a loop and a stem II;
wherein the stem I consists of the sequence of positions 1-21 of SEQ ID NO: 2; the loop consists of the sequence of positions 22-27 of SEQ ID NO: 2; and the stem II consists of the sequence of positions 28-48 of SEQ ID NO: 2.
4 . The method according to claim 3 , wherein in step a), a ratio of the number of the γδ T cells to the number of the tumor cells with reduced FPPS expression and activity is (1-10):1.
5 . The method according to claim 3 , wherein in step b), the vector encoding the chimeric antigen receptor is a lentiviral vector.
6 . A method for tumor immunotherapy, comprising:
a) producing CAR-γδ T cells according to the method of claim 3 ; and
b) transfusing the CAR-γδ T cells into a tumor patient, wherein said CAR-γδ T cells recognize and kill tumor cells in said patient, thereby treating said patient.
7 . The method according to claim 1 , wherein the tumor cells are a chronic myelogenous leukemia cell line.
8 . The method according to claim 7 , wherein the chronic myelogenous leukemia cell line is K562 cells.
9 . The method according to claim 3 , wherein the tumor cells are a chronic myelogenous leukemia cell line.