Concentration measurement device
Provided is a concentration measurement device that includes a light source that emits light, a first optical system that is provided on an optical path of the light emitted from the light source and condenses the light emitted from the light source, a light-transmissive tubular body that is disposed at a position on a rear stage side with respect to a focal position of the first optical system on the optical path and collimates the light incident on a side surface in a state where a fluid flows inside, and a detection unit that detects light through the tubular body.
1 . A concentration measurement device, comprising:
a light source configured to emit light;
a first optical system configured to condense the emitted light, wherein
the first optical system is on an optical path of the emitted light;
a light-transmissive tubular body configured to collimate the condensed light incident on a side surface of the light-transmissive tubular body, wherein
a fluid is inside the light-transmissive tubular body, and
the light-transmissive tubular body is at a position on a rear stage side with respect to a focal position of the first optical system on the optical path; and
a detection unit configured to detect the light through the light-transmissive tubular body.
2 . The concentration measurement device according to claim 1 , wherein
the light-transmissive tubular body has a cylindrical shape, and
the light-transmissive tubular body is further configured to collimate the light that is transmitted through the light-transmissive tubular body in a plane orthogonal to a longitudinal direction of the light-transmissive tubular body.
3 . The concentration measurement device according to claim 1 , further comprising:
a second optical system between the light-transmissive tubular body and the detection unit, wherein
the second optical system is configured to collimate the light transmitted through the light-transmissive tubular body in a plane including a longitudinal direction of the light-transmissive tubular body.
4 . The concentration measurement device according to claim 1 , further comprising:
a light shielding plate between the light-transmissive tubular body and the detection unit, wherein the light shielding plate includes an opening that is through a first surface of the light shielding plate and a second surface of the light shielding plate; and
a third optical system between the light-transmissive tubular body and the light shielding plate, wherein the third optical system is configured to condense the light through the light-transmissive tubular body on the opening.
5 . The concentration measurement device according to claim 4 , wherein the light shielding plate is in contact with an optical path front stage side of the detection unit.
6 . The concentration measurement device according to claim 1 , wherein the fluid contains a biological particle.
7 . The concentration measurement device according to claim 6 , wherein the fluid is a cell suspension stained with an antibody dye.
8 . The concentration measurement device according to claim 1 , wherein the light source is further configured to emit the light in a wavelength band of 700 nm or greater.
9 . The concentration measurement device according to claim 1 , further comprising:
a control unit configured to calculate a concentration of the fluid based on absorbance of the fluid, wherein
the absorbance of the fluid is based on Beer-Lambert law,
the detection unit is further configured to detect the light through the light-transmissive tubular body having a reference fluid, to obtain a first detection result,
the reference fluid has a concentration of 0,
the detection unit is further configured to detect the light through the light-transmissive tubular body having the fluid, to obtain a second detection result, and
the control unit is further configured to calculate the absorbance from the first detection result and the second detection result.