Optimizing the operation of a chromatography system
The present invention relates to a method for purifying a feed comprising at least one target product in a chromatography system having a plurality of purifying units, each having an inlet and an outlet, and a valve assembly having an outlet port and an inlet port. The inlet and the outlet of each purifying unit being connected to a respective port of the valve assembly. The method comprising loading (S 10 ) the plurality of purifying units with feed provided through the inlet port of the valve assembly by sequentially connecting each purifying unit to the inlet port of the valve assembly: eluting (S 12 ) the plurality of purifying units using an elution provided through the inlet port of the valve assembly by sequentially connecting each purifying unit to the inlet port of the valve assembly: and collecting (S 14 ) the at least one target product from the outlet port of the valve.
1 . A method for purifying a feed comprising at least one target product in a chromatography system having a plurality of adsorption purifying units, each purifying unit having an inlet and an outlet, and a valve assembly comprising a single rotary valve, an outlet port, and an inlet port, wherein the inlet and the outlet of each purifying unit is connected to a respective port of the valve assembly, said method comprising:
a) loading the plurality of purifying units with feed provided through the inlet port of the valve assembly by sequentially connecting each purifying unit to the inlet port of the valve assembly;
b) when step a) is completed, eluting the plurality of purifying units using a solution to elute provided through the inlet port of the valve assembly by sequentially connecting each purifying unit to the inlet port of the valve assembly; and
c) collecting the at least one target product from the outlet port of the valve assembly;
wherein the loading step is performed for each of the plurality of purifying units, then the eluting step is performed for each of the plurality of purifying units, and then the collecting step is performed for each of the plurality of purifying units, such that the purification of the feed is a semi-continuous process;
wherein each purifying unit of the chromatography system is a membrane adsorber; and
wherein the plurality of purifying units are connected in parallel.
2 . The method according to claim 1 , wherein the membrane adsorber comprises electrospun material.
3 . The method according to claim 1 , wherein each purifying unit is selected to be a column comprising the membrane adsorber.
4 . The method according to claim 1 , wherein the chromatography system is further provided with a pretreatment device to the inlet of the valve assembly, and the method further comprises conditioning the feed before entering the inlet of the valve assembly.
5 . The method according to claim 4 , wherein the pre-treatment device is selected to be a filter or chromatography column configured to condition the feed by removing selected material.
6 . The method according to claim 1 , wherein the method further comprises when step b) is completed, washing the plurality of purifying units using a solution provided through the inlet port of the valve assembly by sequentially connecting each purifying unit to the inlet port of the valve assembly.
7 . The method according to claim 1 , wherein step b) further comprises, before eluting the plurality of purifying units, washing the plurality of purifying units using a solution provided through the inlet port of the valve assembly by sequentially connecting each purifying unit to the inlet port of the valve assembly.
8 . The method according to claim 1 , wherein steps a)-c) are repeated at least twenty times and during each repeated cycle a part of the same feed is purified using the same purifying units.
9 . The method according to claim 1 , wherein the purifying units are selected to be of the same type.
10 . A chromatography system for purifying a feed comprising at least one target product having a plurality of purifying units, each purifying unit having an inlet and an outlet, and a valve assembly comprising a single rotary valve, an outlet port, and an inlet port, wherein the inlet and the outlet of each purifying unit being connected to a respective port of the valve assembly, said chromatography system further comprises a control unit configured to:
a) load the plurality of purifying units with feed provided through the inlet port of the valve assembly by sequentially connecting each purifying unit to the inlet port of the valve assembly;
b) when step a) completed, elute the plurality of purifying units using a solution to elute provided through the inlet port of the valve assembly by sequentially connecting each purifying unit to the inlet port of the valve assembly; and
c) collect the at least one target product from the outlet port of the valve assembly;
wherein the loading step is performed for each of the plurality of purifying units, then the eluting step is performed for each of the plurality of purifying units, and then the collecting step is performed for each of the plurality of purifying units, such that the purification of the feed is a semi-continuous process;
wherein each purifying unit of the chromatography system is a membrane adsorber; and
wherein the plurality of purifying units are connected in parallel.