Measurement sample preparation method, analysis method, reagent, and reagent kit
Disclosed is a preparation method for preparing a measurement sample comprising an aggregate of metal nanoparticles having an analyte bound thereto, the preparation method comprising: contacting the analyte with a linker to bind the analyte to the linker; and contacting the linker that has been bound to the analyte with the metal nanoparticles to bind the linker to the metal nanoparticles.
1 . An analysis method for analyzing analytes in a sample using a spectroscopic analysis, the method comprising:
preparing a measurement sample comprising, wherein the preparing comprises:
contacting the analytes with linkers to bind the analytes to the linkers, whereby a plurality of complexes are formed, each complex having the analyte and the linker bound to the analyte;
contacting the complexes with metal nanoparticles to bind the complexes to the metal nanoparticles, whereby a plurality of first aggregates are formed in a solution, each first aggregate having the metal nanoparticle and the complex bound to the metal nanoparticle; and
adding an inorganic salt or an acid to the solution containing the plurality of the first aggregates, whereby a second aggregate is formed, the second aggregate consists of a plurality of the analytes, a plurality of the linkers and a plurality of the metal nanoparticles;
obtaining an optical spectrum from the second aggregates as a measurement sample; and
outputting information about a type of the analyte in the second aggregate on the basis of the obtained optical spectrum,
wherein the analyte is at least one substance selected from the group consisting of a single amino acid, a nucleic acid, a catecholamine, a polyamine, an organic acid, an extracellular vesicle and a virus.
2 . The analysis method according to claim 1 , wherein the analyte has a functional group, and the linker has a reactive group capable of reacting with the functional group.
3 . The analysis method according to claim 2 , wherein the functional group is at least one selected from the group consisting of an amino group, a carboxyl group and a hydroxyl group.
4 . The analysis method according to claim 3 , wherein the functional group is an amino group and the reactive group is at least one selected from the group consisting of an N-hydroxysuccinimide ester group, an isothiocyanate group, an isocyanate group, an acyl azide group, a sulfonyl chloride group, an aldehyde group, an imide ester group, a fluorobenzene group, an epoxide group, a carbodiimide group, a carbonate group, and a fluorophenyl ester group.
5 . The analysis method according to claim 4 , wherein the reactive group is an N-hydroxysuccinimide ester group, and the linker is at least one component selected from the group consisting of dithiobis(succinimidyl propionate), dithiobis(succinimidyl undecanoate), dithiobis(succinimidyl octanoate) and dithiobis(succinimidyl hexanoate).
6 . The analysis method according to claim 1 , wherein the metal nanoparticle is a nanoparticle, the nanoparticle being at least one metal selected from the group consisting of gold, silver, platinum, copper and palladium.
7 . The analysis method according to claim 1 , wherein a particle diameter of the metal nanoparticle is equal or greater than 10 nm and equal or smaller than 150 nm.
8 . The analysis method according to claim 1 , wherein the sample is blood, serum, plasma, saliva or a body fluid, or is a solution of the analytes in which water or a buffer solution is used as a solvent.
9 . The analysis method according to claim 1 , wherein the spectroscopic analysis is a surface enhanced Raman scattering analysis.