Granted Patent
B2
US 12,618,837 · App. 17/596,950 · Granted May 5, 2026
Method of identifying pro-inflammatory dendritic cells
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Abstract
There is provided a method of identifying pro-inflammatory dendritic cells, the method comprising: determining an expression of CD5, CD14 and/or CD163 in cells, wherein CD5 − , CD14 + and/or GD163 + cells are identified as pro-inflammatory dendritic cells. Also disclosed is a method of characterising inflammation and/or inflammatory disease in a subject, the method comprising: determining a proportion of CD5 − , CD14 + and/or GD163 + dendritic cells in the subject's sample, wherein the proportion positively correlates with the level of inflammation and/or the severity of inflammatory disease in the subject.
Claims (19)
1 . A method of characterising CD1c + dendritic cells, the method comprising:
determining an expression of one or more of CD5, CD14 and CD163 in the dendritic cells,
wherein where the dendritic cells are determined to be CD5−, CD14+ and CD163+, identifying the dendritic cells as pro-inflammatory dendritic cells.
2 . The method according to claim 1 , wherein where the dendritic cells are determined to be CD163+CD14+, identifying the dendritic cells as highly pro-inflammatory dendritic cells that are more pro-inflammatory than CD163− or CD14− dendritic cells.
3 . The method according to claim 1 , the method further comprising determining a ratio of CD163+CD14+ dendritic cells to a total number of dendritic cells in a sample.
4 . The method according to claim 1 , the method further comprising determining an expression of one or more of CD11b, CD36, CD64, CD87, CD107a, CD206, CD274, CD354, FcεRIα, HLA-DQ, CD2, CD59, CD81, CD166, CD229, CD271 and Integrin β7 in the dendritic cells.
5 . The method according to claim 1 , wherein the dendritic cells have one or more of the following properties:
(i) is a conventional CD1c + dendritic cell 2 (cDC2);
(ii) is dependent on IRF4 for differentiation;
(iii) is dependent on KLF4 for differentiation;
(iv) is dependent on FLT3 ligand (FLT3L) for differentiation; and
(v) is capable of activating and/or polarizing T cells.
6 . The method according to claim 1 , the method further comprising determining a ratio of CD163+CD14+ dendritic cells to a total number of dendritic cells having one or more of the properties selected from the group consisting of:
(i) a conventional dendritic cell;
(ii) dependent on IRF4 for differentiation;
(iii) dependent on KLF4 for differentiation;
(iv) dependent on FLT3 ligand (FLT3L) for differentiation; and
(v) capable of activating and/or polarizing T cells.
7 . A kit for characterising CD1c + dendritic cells, inflammation and/or inflammatory disease, the kit comprising reagents for detecting CD5, CD14 and CD163, wherein the kit further comprises methods for identifying dendritic cells to be pro-inflammatory dendritic cells where the dendritic cells are determined to be CD5−, CD14+ and CD163+.
Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Dec 22, 2021
From: GINHOUX, FLORENT; DUTERTRE, CHARLES ANTOINE
To: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
Reel/Frame 058461/0801 →
Priority Claims (1)
SG 10201905956V
· Jun 26, 2019
· national
Continuity (1)
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