IP Library › Granted Patent US 12,630,526
Granted Patent B2
US 12,630,526 · App. 17/609,856 · Granted May 19, 2026

Processes for preparing aminopyrimidine compounds

Inventors: Manmohan Reddy Leleti (Dublin, CA); Dillon Harding Miles (Berkeley, CA); Brandon Reid Rosen (San Mateo, CA); Ehesan Ul Sharif (Menlo Park, CA); Jay Patrick Powers (Pacifica, CA)
Assignee: ARCUS BIOSCIENCES, INC.
C07D401/14C07D239/42
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Quick Facts
Patent No.
US 12,630,526
App. No.
17/609,856
Granted
May 19, 2026
Kind
B2
Abstract

Provided herein are improved processes for preparing aminopyrimidine compounds of Formula (I). The disclosed processes advantageously proceed through a β-diketoester intermediate of Formula (A) and avoid the direct linking of a pyrimidine and phenyl moieties. The disclosed methods significantly increase yield of the desired compounds and simplifies the synthetic route.

Claims (47)

1 . A process for preparing a compound of Formula (IIa):

said process comprising:

a′) contacting a compound of Formula (A)

wherein

R 1a is CH 3 ;

R 2a is CN;

R 1b , R 2b , and R 3 are each H;

R 4 is selected from the group consisting of C1-C8 alkyl and C3-C8 cycloalkyl, each of which is optionally substituted with from 1 to 6 members independently selected from the group consisting of F and Cl;

with a guanidine reagent in 2,2,2-trifluoroethanol to obtain a compound of Formula (B)

wherein said guanidine reagent is guanidine HCl or guanidine carbonate;

and contacting the compound of Formula (B) with a halogenation agent to form a compound of Formula (Ia)

 wherein X is Cl or Br and the halogenation agent is POCl 3 , PSCl 3 , SOCl 2 , (COCl) 2 , PCl 5 , PBr 3 , N-chlorosuccinimide (NCS), or N-bromosuccinimide (NBS);

b′) converting said compound of Formula (Ia) to a compound of Formula (Ca);

and

c′) combining said compound of Formula (Ca) with a compound of Formula (D),

to produce said compound of Formula (IIa).

2 . The process according to claim 1 , wherein step b′) comprises

b′-1) contacting the compound of Formula (Ia) with triisopropylsilylacetylene under Sonogashira coupling conditions to produce an intermediate of Formula (Ca′):

and

b′-2) contacting the compound of Formula (Ca′) with a desilylating reagent to produce said compound of Formula (Ca).

3 . The process according to claim 2 , wherein said Sonogashira coupling conditions comprise a palladium catalyst and a copper co-catalyst.

4 . The process according to claim 2 , wherein said Sonogashira coupling conditions further comprise an amine base.

5 . The process according to claim 4 , wherein the amine base is triethylamine or N,N-diisopropylethylamine.

6 . The process according to claim 2 , wherein said desilylating reagent is selected from the group consisting of tetrabutylammonium fluoride (TBAF), tetrabutylammonium hydroxide (TBAH), sodium tetrachloroaurate (III), and acetyl chloride.

7 . The process according to claim 1 , wherein step c′) further comprises a copper catalyst.

8 . The process in accordance with claim 1 , wherein R 4 is selected from the group consisting of methyl, ethyl, isopropyl, tert-butyl, and 2,2,2-rifluoroethyl.

9 . The process in accordance with claim 1 , wherein contacting the compound of Formula (B) with a halogenation agent is carried out in a polar aprotic organic solvent.

10 . The process in accordance with claim 9 , wherein said polar aprotic organic solvent is selected from the group consisting of acetonitrile, DMF, DMSO, NMP, and combinations thereof.

11 . The process in accordance with claim 9 , wherein the halogenation agent is POCl 3 ; X is Cl; said guanidine reagent is guanidine carbonate; and said polar aprotic organic solvent is acetonitrile.

12 . The process in accordance with claim 1 , wherein contacting the compound of Formula (B) with a halogenation agent is conducted at a temperature of about 67-83° C.

13 . The process in accordance with claim 1 , wherein said compound of Formula (A), said guanidine reagent, and 2,2,2-trifluoroethanol are heated to reflux temperature.

14 . The process in accordance with claim 1 , wherein, prior to contacting the compound of Formula (B) with a halogenation agent, said compound of Formula (B) is triturated with an organic solvent to remove a byproduct of Formula (i)

15 . The process in accordance with claim 14 , wherein said organic solvent is selected from the group consisting of ethanol, methanol, acetonitrile, dichloromethane, ethyl acetate, and methyl tert-butyl ether.

16 . The process in accordance with claim 9 , wherein contacting the compound of Formula (B) with a halogenation agent is conducted with a base additive.

17 . The process in accordance with claim 16 , wherein said base additive is selected from the group consisting of trimethylamine, diisopropylethylamine, dimethylaniline, benzyltrimethylammonium chloride, and benzyltriethylammonium chloride.

18 . The process in accordance with claim 1 , wherein the compound of Formula (A) is prepared by:

i) contacting a compound of Formula (a)

with a halogenation agent to prepare a compound of Formula (b)

wherein

Y is a halogen; and

ii) combining the compound of Formula (b) with a compound of Formula (c)

in the presence of a base to form a compound of Formula (A).

19 . The process in accordance with claim 18 , wherein the compound of Formula (a) is prepared by:

i) contacting a compound of Formula (a′y)

with a cyanation reagent to prepare a compound of Formula (a′x)

or

ii) contacting the compound of Formula (a′y) with a Grignard reagent and CO 2 to give the compound of Formula (a′x).

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Oct 21, 2022
From: LELETI, MANMOHAN REDDY; MILES, DILLON HARDING; ROSEN, BRANDON REID; SHARIF, EHESAN UL; POWERS, JAY PATRICK
To: ARCUS BIOSCIENCES, INC.
Reel/Frame 061492/0206 →
Continuity (2)
Provisional Application 62858050 · Jun 6, 2019
Related Publication 20220235031A1 · Jul 28, 2022
References Cited (28)
US 2778830A · Pasedach et al. · 1957 [cited by applicant]
US 7737153B2 · Feurer · 2010 [cited by examiner]
US 10399962B2 · Beatty et al. · 2019 [cited by applicant]
US 11072597B2 · Beatty et al. · 2021 [cited by applicant]
US 11478479B2 · Karakunnel · 2022 [cited by applicant]
US 11993584B2 · Jeffrey et al. · 2024 [cited by applicant]
US 12195447B2 · Beatty et al. · 2025 [cited by applicant]
US 20220235031A1 · Leleti et al. · 2022 [cited by applicant]
US 20230338377A1 · Karakunnel · 2023 [cited by applicant]
WO WO9744326A1 · 1997 [cited by examiner]
WO WO02083652 · 2002 [cited by applicant]
WO WO2004104007 · 2004 [cited by applicant]
WO WO2010013222 · 2010 [cited by applicant]
WO WO2012149157 · 2012 [cited by applicant]
WO 2018136700A1 · 2018 [cited by applicant]
Khaksar “Fluorinated alcohols: A magic medium for the synthesis of heterocyclic compounds” Journal of Fluorine Chemistry 172 (2015) 51-61. [cited by examiner]
Zhang, M. “Carboxylic acid esters: synthesis with retention of the functional group” Science of Synthesis (2006), 20b, 863-946. [cited by examiner]
International Search Report mailed Sep. 4, 2020 corresponding to PCT/US2020/036379 filed Jun. 5, 2020; 3 pages. [cited by applicant]
Written Opinion of the ISA mailed Sep. 4, 2020 corresponding to PCT/US2020/036379 filed Jun. 5, 2020; 5 pages. [cited by applicant]
Jansa, Petr et al., “5-Substituted 2-amino-4,6-dihydroxypyrimidines and 2-amino-4, 6-dichloropyrimidines: synthesis and inhibitory effects on immune-activated nitric oxide production,” [cited by applicant]
Rosen, Brandon R. et al., “Improved synthesis of sterically encumbered heteroaromatic biaryls from aromatic β-keto esters,” [cited by applicant]
Benderitter et al., 2-Amino-6-iodo-4-tosyloxypyrimidine: a versatile key intermediate for regioselective functionalization of 2-aminopyrimidines in 4- and 6-positions, Tetrahedron 2007, 63, pp. 12465-12470. [cited by applicant]
B-Rao et al., Identification of novel isocytosine derivatives as xanthine oxidase inhibitors from a set of virtual screening hits, Bioorganic & Medicinal Chemistry 2012, vol. 20, pp. 2930-2939, XP028412821. [cited by applicant]
Dandia et al. 2,2,2-Trifluoroethanol as Green Solvent in Organic Synthesis: A Review. Mini-Reviews in Organic Chemistry, 2014, 11, 462-476. [cited by applicant]
Extended European Search Report for European Patent Application No. 20819053.8 dated Apr. 6, 2023. 9 pages. [cited by applicant]
Santos et al., Anti-parasitic Guanidine and Pyrimidine Alkaloids from the Marine Sponge Monanchora arbuscula, J Nat Prod. 2015;78(5):1101-1112. [cited by applicant]
Sharif et al., Development of a Scalable and Practical Synthesis of AB928, a Dual A2a/A2b Receptor Antagonist, Organic Process Research & Development 2020, 24, pp. 1254-1261. [cited by applicant]
Wang et al., Ambient-Light-Promoted Three-Component Annulation: Synthesis of Perfluoroalkylated Pyrimidines, Org. Lett. 2017, 19, pp. 2358-2361, XP093035640. [cited by applicant]