Granted Patent B2

US 12,630,831 · App. 17/036,257 · Granted May 19, 2026

Inventors: Roee Amit (Tel Aviv, IL); Noa Katz (Tirat Carmel, IL)

Assignee: TECHNION RESEARCH & DEVELOPMENT FOUNDATION LIMITED

C12N15/67C07H21/00C12P21/02C12N15/1037C12N15/115

Abstract

Claims (12)

1 . A synthetic RNA molecule, comprising at least 5 different RNA-binding protein (RBP)-binding motifs, wherein said at least 5 RBP-binding motifs (1) bind the same RBP selected from a phage coat protein selected from PP7 phage coat protein (PCP), QB phage coat protein (QCP) and MS2 phage coat protein (MCP) and (2) comprise non-identical sequences, wherein

said RBP is QCP and the at least 5 RBP-binding motifs are QCP-binding motifs selected from the group consisting of SEQ ID NOs 3-102 wherein at least one QCP-binding motif is selected from the group consisting of SEQ ID NOs 3-9, and wherein each non-identical sequence of the at least 5 QCP-binding motifs comprises at least 5 nucleotide differences from all other QCP-binding motifs in the synthetic RNA molecule;

said RBP is MCP and the at least 5 RBP-binding motifs are MCP-binding motifs selected from the group consisting of SEQ ID NOs 103-202 wherein at least one MCP-binding motif is selected from the group consisting of SEQ ID NOs 103-108 and 110, and wherein each non-identical sequence of the at least 5 MCP-binding motifs comprises at least 5 nucleotide differences from all other MCP-binding motifs in the synthetic RNA molecule; or

said RBP is PCP and the at least 5 RBP-binding motifs are PCP-binding motifs selected from the group consisting of SEQ ID NOs 203-302 wherein at least one PCP-binding motif is selected from the group consisting of SEQ ID NOs 203-204 and 206-210, and wherein each non-identical sequence of the at least 5 PCP-binding motifs comprises at least 5 nucleotide differences from all other PCP-binding motifs in the synthetic RNA molecule.

2 . The synthetic RNA molecule of claim 1 , wherein said at least 5 different RBP-binding motifs comprise at least 10 different RBP-binding motifs that all bind the same phage coat protein and all comprise non-identical sequences.

3 . The synthetic RNA molecule of claim 1 , further comprising at least two different RBP-binding motifs to a second RBP, wherein said RBP and said second RBP are different phage coat proteins selected from PCP, QCP and MCP, and wherein said at least two different RBP-binding motifs to the second RBP comprise a second set of at least 2 non-identical sequences and wherein each RBP-binding motif of said second set of at least 2 non-identical sequences comprises at least 5 nucleotide differences from all other RBP-binding motifs of said second set.

4 . The synthetic RNA molecule of claim 3 , comprising at least 5 second RBP-binding motifs that bind said second RBP and comprise a second set of at least 5 non-identical sequences, wherein each RBP-binding motif of said second set of at least 5 non-identical sequences comprises at least 5 nucleotide differences from all other RNA-binding motifs of said second set.

5 . The synthetic RNA molecule of claim 3 , wherein said at least 5 RBP-binding motifs and said at least two second RBP-binding motifs do not overlap with each other.

6 . The synthetic RNA molecule of claim 3 , further comprising at least two different RBP-binding motifs to a third RBP wherein said at least two different RBP-binding motifs to a third RBP comprise a third set of at least 2 non-identical sequences and wherein each RBP-binding motif of said third set of at least 2 non-identical sequences comprises at least 5 nucleotide differences from all other RBP-binding motifs of said third set, and wherein said RBP is PCP, said second RBP is QCP and said third RBP is MCP.

7 . The synthetic RNA molecule of claim 1 , wherein said synthetic RNA molecule does not encode a protein.

8 . The synthetic RNA molecule of claim 7 , wherein said synthetic RNA molecule does not comprise a microRNA (miR) or small interfering RNA (siRNA).

9 . The synthetic RNA molecule of claim 8 , comprising at least 5 third RBP-binding motifs that bind said third RBP and comprise a third set of at least 5 non-identical sequences, wherein each RBP-binding motif of said third set of at least 5 non-identical sequences comprises at least 5 nucleotide differences from all other RBP-binding motifs of said third set.

Assignments (1)

Continuity (2)

References Cited (73)