Granted Patent
B2
US 12,637,503 · App. 18/051,456 · Granted May 26, 2026
Antigen-binding molecules that bind to
Assignee:
K-Bio Investment, LLC
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Abstract
Antigen-binding molecules (ABMs) that bind to Porphyromonas gingivalis are described. The ABMs may be human or humanized ABMs. The ABMs find use in treating infections involving P. gingivalis , such as periodontal disease. Also provided are methods of treating or preventing a disorder or disease by administering the ABM.
Claims (41)
1 . An antigen-binding molecule that binds to Porphyromonas gingivalis , wherein the antigen-binding molecule comprises:
a heavy chain comprising a heavy chain variable region (HVR) comprising an HVR complementarity determining region (HCDR) 1, an HCDR2, and an HCDR3 from the sequence of SEQ ID NO:9 or 37 per the Kabat or Chothia definition of CDRs; and
a light chain comprising a light chain variable region (LVR) comprising an LVR complementarity determining region (LCDR) 1, an LCDR2, and an LCDR3 from the sequence of SEQ ID NO:10 or 38 per the Kabat or Chothia definition of CDRs;
wherein the heavy chain comprises an amino acid other than lysine at position 105 as numbered according to the numbering as provided in the sequence of SEQ ID NO: 172; and
wherein the heavy chain comprises an amino sequence at least 80% identical to the sequence of SEQ ID NO:263 and the light chain comprises an amino sequence at least 80% identical to the sequence of SEQ ID NO:253.
2 . The antigen-binding molecule of claim 1 , wherein the HCDR1 comprises the sequence of SEQ ID NO:3, the HCDR2 comprises the sequence of SEQ ID NO: 4, the HCDR3 comprises the sequence of SEQ ID NO:5, the LCDR1 comprises the sequence of SEQ ID NO:6, the LCDR2 comprises the sequence of SEQ ID NO:7, and the LCDR3 comprises the sequence of SEQ ID NO:8.
3 . The antigen-binding molecule of claim 1 , wherein the HVR comprises the sequence of SEQ ID NO:37 and the LVR comprises the amino acid sequence of SEQ ID NO:38.
4 . The antigen-binding molecule of claim 1 , wherein the antigen-binding molecule comprises at least one of:
one or more HVR residues selected from L48, L67, K71, V78, and M92, as numbered according to the numbering as provided in the sequence of SEQ ID NO:37, and
one or more LVR residues selected from Q46, W48, A61, Y72, and T86, as numbered according to the numbering as provided in the sequence of SEQ ID NO:38.
5 . The antigen-binding molecule of claim 1 , wherein the HVR comprises an amino acid sequence at least 80% identical to the sequence of any one of SEQ ID NOs: 29-32 and the LVR comprises an amino acid sequence at least 80% identical to the sequence of any one of SEQ ID NOs: 33-36.
6 . The antigen-binding molecule of claim 1 , wherein the HVR comprises the sequence of any one of SEQ ID NOs: 29-32 and the LVR comprises the sequence of any one of SEQ ID NOs: 33-36.
7 . The antigen-binding molecule of claim 1 , wherein the HVR comprises the sequence of SEQ ID NO:30 and the LVR comprises the sequence of SEQ ID NOs: 33.
8 . The antigen-binding molecule of claim 1 , wherein the HVR comprises the sequence of SEQ ID NO:30 and the LVR comprises the sequence of SEQ ID NO: 35.
9 . The antigen-binding molecule of claim 1 , wherein the antigen-binding molecule is humanized.
10 . The antigen-binding molecule of claim 1 , wherein the amino acid other than lysine at position 105 as numbered in the sequence of SEQ ID NO:172 is an alanine.
11 . An antigen-binding molecule that binds to Porphyromonas gingivalis , wherein the antigen-binding molecule comprises a heavy chain and a light chain, wherein the heavy chain comprises the sequence of any one of SEQ ID NOs: 245, 247, 249, 251, 261, 263, 265, and 267 and the light chain comprises the sequence of any one of SEQ ID NOs: 253, 255, 257, and 259.
12 . An antigen-binding molecule that binds to Porphyromonas gingivalis , wherein the antigen-binding molecule comprises a heavy chain and a light chain, wherein the heavy chain comprises the sequence of SEQ ID NO:263 and the light chain comprises the sequence of SEQ ID NO:253.
13 . The antigen-binding molecule of claim 12 , wherein the heavy chain comprises the sequence of SEQ ID NO:239 and the light chain comprises the sequence of SEQ ID NO:229.
14 . An antigen-binding molecule that binds to Porphyromonas gingivalis , wherein the antigen-binding molecule comprises a heavy chain and a light chain, wherein the heavy chain comprises the sequence of SEQ ID NO:263 and the light chain comprises the sequence of SEQ ID NO:357.
15 . The antigen-binding molecule of claim 14 , wherein the heavy chain comprises the sequence of SEQ ID NO:239 and the light chain comprises the sequence of SEQ ID NO:233.
16 . A pharmaceutical composition comprising the antigen-binding molecule of claim 1 , and a pharmaceutically acceptable carrier.
17 . A pharmaceutical composition comprising the antigen-binding molecule of claim 11 , and a pharmaceutically acceptable carrier.
18 . A nucleic acid encoding the antigen-binding molecule of claim 1 .
19 . A nucleic acid encoding the antigen-binding molecule of claim 11 .
20 . A vector comprising the nucleic acid of claim 18 .
21 . A vector comprising the nucleic acid of claim 19 .
22 . A cell comprising a nucleic acid encoding the antigen-binding molecule of claim 1 , or a vector comprising the nucleic acid.
23 . A cell comprising a nucleic acid encoding the antigen-binding molecule of claim 11 , or a vector comprising the nucleic acid.
24 . A method of treating a condition, disorder, or disease associated with a P. gingivalis infection in a subject in need thereof, the method comprising administering to the subject a therapeutically effective amount of the antigen-binding molecule of claim 1 .
25 . A method of treating a condition, disorder, or disease associated with a P. gingivalis infection in a subject in need thereof, the method comprising administering to the subject a therapeutically effective amount of the antigen-binding molecule of claim 11 .
26 . The method of claim 24 , wherein the condition, disorder, or disease associated with a P. gingivalis infection is an age-related cognitive disorder.
27 . The method of claim 26 , wherein the age-related cognitive disorder is dementia or Alzheimer's disease.
28 . The method of claim 24 , wherein the condition, disorder, or disease associated with a P. gingivalis infection is a vascular disease.
29 . The method of claim 28 , wherein the vascular disease is cardiovascular disease, atherosclerosis, coronary artery disease, myocardial infarction, stroke, or cardiac hypertrophy.
30 . The method of claim 24 , further comprising administering to the subject at least one other therapeutic agent for treating the condition, disorder, or disease associated with a P. gingivalis infection.
31 . The method of claim 25 , wherein the condition, disorder, or disease associated with a P. gingivalis infection is an age-related cognitive disorder.
32 . The method of claim 31 , wherein the age-related cognitive disorder is dementia or Alzheimer's disease.
33 . The method of claim 25 , wherein the condition, disorder, or disease associated with a P. gingivalis infection is a vascular disease.
34 . The method of claim 33 , wherein the vascular disease is cardiovascular disease, atherosclerosis, coronary artery disease, myocardial infarction, stroke, or cardiac hypertrophy.
35 . The method of claim 25 , further comprising administering to the subject at least one other therapeutic agent for treating the condition, disorder, or disease associated with a P. gingivalis infection.
Assignments (2)
FORECLOSURE AND SECURED PARTY AFFIDAVIT OF DEFAULT AND TRANSFER OF ASSETS
Recorded Apr 30, 2024
From: KEYSTONE BIO, INC.
To: K-BIO INVESTMENT, LLC
Reel/Frame 067274/0140 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Nov 10, 2022
From: NARA, PETER L.; SINDELAR, DANIEL L.
To: KEYSTONE BIO, INC.
Reel/Frame 061725/0287 →
Continuity (10)
Continuation In Part
PCTUS2021057758
· Nov 2, 2021
Provisional Application
63364592
· May 12, 2022
Provisional Application
63364182
· May 4, 2022
Provisional Application
63231964
· Aug 11, 2021
Provisional Application
63225295
· Jul 23, 2021
Provisional Application
63221405
· Jul 13, 2021
Provisional Application
63208873
· Jun 9, 2021
Provisional Application
63135878
· Jan 11, 2021
Provisional Application
63109286
· Nov 3, 2020
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