IP Library › Granted Patent US 12,643,103
Granted Patent B2
US 12,643,103 · App. 17/802,661 · Granted Jun 2, 2026

Systems, devices and methods for multiplexed analysis

Inventors: Benjamin Ports (Guilford, CT); Igor Nikonorov (Whitestone, NY); Peter Tsiomplikas (Bridgeport, CT); Michael Kane (Branford, CT); Sergei Ivani (Branford, CT); Patrick Paczkowski (East Haven, CT); Luka Djapic (Branford, CT)
Assignee: IsoPlexis Corporation
B01L3/502761B01L2200/0647B01L2300/044B01L2400/0481B01L2400/082
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Quick Facts
Patent No.
US 12,643,103
App. No.
17/802,661
Granted
Jun 2, 2026
Kind
B2
Abstract

Embodiments of the current disclosure are directed to systems, methods and apparatus for the multiplexed analysis of biological material. In some embodiments, the apparatus may comprise an assembly including a first frame including a plurality of first openings; a capture agent slide; and a channel membrane.

Claims (71)

1 . A multiplex assay device (MAD) configured for at least one of multiplexed analysis of biological material and a cell suspension incubator, the MAD comprising an assembly including:

a first frame including a plurality of first openings arranged in a plurality of rows, wherein each first opening extends from a first side of the first frame to a second side of the first frame;

a capture agent (CA) slide, wherein a plurality of capture agents are attached to a surface of the CA slide;

a channel membrane configured with:

a plurality of elongated slots configured as channels, each extending substantially from a first end of the channel membrane to a second end of the channel membrane,

with a first side for positioning adjacent the first frame, and

a second side to overlay the CA slide such that capture agents attached to the surface of the CA slide are within each channel of the plurality of channels;

and

a second frame;

wherein the first frame is configured to couple with the second frame such that the CA slide and channel membrane are arranged therebetween; and

each channel of the channel membrane is positioned below a respective first opening of the plurality of first openings such that a biological sample loaded into a respective first opening proliferates along at least a portion of the respective channel to interact with capture agents of the CA slide.

2 . The device of claim 1 , wherein the first frame is configured to removably couple with the second frame.

3 . The device of claim 1 , further comprising a cover membrane configured to cover the plurality of first openings.

4 . The device of claim 3 , wherein the cover membrane is configured to cover the first openings after a biological material sample has been pipetted into at least one of the first openings.

5 . The device of claim 1 , wherein each first opening includes identifiable indicia.

6 . The device of claim 1 , wherein at least one of the first openings in each row corresponds to a designated background opening (BO) for receiving background medium.

7 . The device of claim 1 , further comprising at least one capillary stop arranged adjacent at least one of the plurality of first openings.

8 . The device of claim 1 , further comprising at least one capillary stop arranged adjacent at least one of the plurality of first openings, wherein the at least one capillary stop is configured to prevent cross-contamination between adjacent first openings.

9 . The device of claim 1 , further comprising at least one capillary stop arranged adjacent at least one of the plurality of first openings, wherein the at least one capillary stop is configured to prevent cross-contamination between at least one first opening of a first row of the plurality of rows and at least one first opening of a second row of the plurality of rows adjacent the first row.

10 . The device of claim 1 , further comprising at least one input opening and at least one output opening.

11 . The device of claim 10 , wherein the at least one input opening is arranged on an end of the first frame.

12 . The device of claim 10 , wherein the at least one input opening extends from the first side of the first frame to the second side of the first frame.

13 . The device of claim 10 , wherein the at least one input opening is configured for receiving a flow.

14 . The device of claim 10 , wherein the at least one output opening is arranged on an end of the first frame.

15 . The device of claim 10 , wherein the at least one output opening extends from the first side of the first frame to the second side of the first frame.

16 . The device of claim 10 , wherein the at least one output opening is configured for exhausting a flow.

17 . The device of claim 1 , further comprising at least one flexible seal.

18 . The device of claim 10 , further comprising a flexible seal provided for the at least one input opening.

19 . The device of claim 10 , further comprising a pair of flexible seals, one each for sealing the at least one input opening and the at least one output opening.

20 . The device of claim 19 , further comprising a respective opening or recess arranged on the first frame for receiving a respective flexible seal of the pair of flexible seals.

21 . The device of claim 19 , wherein the first flexible seal of the pair of flexible seals is provided at a first end of the first frame, and the second flexible seal of the pair of flexible seals is provided at a second, opposite end of the first frame.

22 . The device of claim 1 , wherein the second frame includes an opening so as to image the CA slide and channels established by the channel membrane facing thereto.

23 . The device of claim 10 , wherein the first frame includes a plurality of passages connecting the at least one input to the at least one output via the plurality of channels of the channel membrane so as to establish a serpentine, serial channel.

24 . The device of claim 10 , wherein the first frame includes a plurality of passages connecting the at least one input to the at least one output via the plurality of channels of the channel membrane so as to establish a serpentine, serial channel, and wherein the plurality of passages includes a first passage connecting the at least one input to an end of the first channel of the channel membrane.

25 . The device of claim 10 , wherein the first frame includes a plurality of passages connecting the at least one input to the at least one output via the plurality of channels of the channel membrane so as to establish a serpentine, serial channel, and wherein the plurality of passages includes a second passage connecting the at least one output to an end of the last channel of the channel membrane.

26 . The device of claim 10 , wherein the first frame includes a plurality of third passages each for connecting every other adjacent end of adjacent channels such that the serpentine channel is established from the at least one input, serially through each channel, and optionally, to the at least one output.

27 . A multiplex assay device (MAD) configured for multiplexed analysis of biological material comprising an assembly including:

a first frame including:

a plurality of first openings arranged in a plurality of rows, wherein:

each first opening including identifiable indicia and each extending from a first side of the first frame to a second side of the first frame,

and

each row including a designated background opening (BO) for receiving background medium;

a plurality of capillary stops arranged adjacent each of the plurality of first openings configured to prevent cross-contamination between at least one first opening of a first row of the plurality of rows and at least one first opening of a second row of the plurality of rows adjacent the first row,

at least one input opening arranged on a first end of the first frame and extending from the first side of the first frame to the second side of the first frame and configured for receiving a flow,

and

at least one output opening arranged on a second end of the frame opposite the first end and extending from the first side of the frame to the second side of the frame and configured for exhausting the flow;

a first membrane configured to cover the plurality of first openings after a biological material sample has been pipetted into at least one of the first openings;

a capture agent (CA) slide;

a channel membrane configured:

with a plurality of elongated slots configured as channels, each extending substantially from a first end of the channel membrane to a second end of the channel membrane, the channels including a first channel and a last channel, with a first side for positioning adjacent the first frame,

and

a second side to overlay the CA slide such that capture agents contained on the slide are within each channel of the plurality of channels,

a second frame;

a pair of flexible seals, each one provided for the at least one input opening and the at least one output opening, at a first end and a second end of the assembly adjacent or within a recess of the second housing or frame;

and

a coded label for identifying the MAD,

wherein:

the first frame is configured to removably couple with the second frame such that the CA slide and channel membrane are arranged therebetween,

the second frame includes an opening so as to image the side of the CA slide and channels established by the channel membrane facing thereto,

each channel of the channel membrane being positioned below at least one first opening of each row of first openings, such that a sample loaded into a respective first opening proliferates along at least a portion of the channel to interact with capture agents of the slide,

and

a plurality of passages connecting the at least one input to the at least one output via the plurality of channels of the channel membrane so as to establish a serpentine, serial channel, the plurality of passages including:

a first passage connecting the at least one input to an end of the first channel of the channel membrane,

a second passage connecting the at least one output to an end of the last channel of the channel membrane,

and

a plurality of passages each for connecting every other adjacent end of adjacent channels such that the serpentine channel is established from the at least one input, serially through each channel to the at least one output.

28 . A multiplex assay system configured for multiplexed analysis of biological material, the system comprising:

a receiving area configured to receive a plurality of multiplex assay devices (MADs) of claim 1 ;

a fluorescing device configured to expose at least one of the capture agent slides and corresponding channels of the channel membrane to fluorescing light;

and

an imager configured to image at least one of the capture agent slides and corresponding channels of the channel membrane upon the capture agent slides and channels being exposed to the fluorescing light.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Oct 4, 2022
From: PORTS, BENJAMIN; NIKONOROV, IGOR; TSIOMPLIKAS, PETER; KANE, MICHAEL; IVANI, SERGEI; PACZKOWSKI, PATRICK; DJAPIC, LUKA
To: ISOPLEXIS CORPORATION
Reel/Frame 061305/0874 →
Continuity (2)
Provisional Application 62982472 · Feb 27, 2020
Related Publication 20230191409A1 · Jun 22, 2023
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