Granted Patent
B2
US 12,643,926 · App. 17/324,354 · Granted Jun 2, 2026
LysM receptor motifs
Inventors:
Elena Simona Radutoiu (Aarhus, DK); Kasper Røjkjær Andersen (Aarhus, DK); Jens Stougaard Jensen (Aarhus, DK); Damiano Lironi (Aarhus, DK); Christina Krönauer (Aarhus, DK); Mette Laursen (Aarhus, DK)
Assignee:
Aarhus Universitet
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Abstract
Aspects of the present disclosure relate to genetically altered LysM receptors. In particular, the present disclosure relates replacement of part or all of motifs in the LysM1 domain with the corresponding motifs of the LysM1 domain from a donor LysM receptor that can alter the affinity, selectivity, and/or specificity for an oligosaccharide, particularly for Nod factors (lipochitooligosaccharides (LCOs)). The present disclosure also relates to genetically altering LysM receptors in plants to include a modified LysM1 domain and to genetically altering LysM receptors in plants by replacement of part or all of motifs in the LysM1 domain. The present disclosure further relates to combining LysM1 domain modifications with modifications of LysM2 domains to include a hydrophobic patch or alter the hydrophobic patch, whereby the LysM2 domain modifications can alter the affinity, selectivity, and/or specificity for an oligosaccharide, particularly for Nod factors (lipochitooligosaccharides (LCOs)).
Claims (15)
1 . A method of making a modified plant LYK3 or CERK6 LysM receptor polypeptide comprising modifying a nucleic acid encoding a wild-type plant LYK3 or CERK6 LysM receptor polypeptide to produce a modified nucleic acid that encodes the modified plant LYK3 or CERK6 LysM receptor polypeptide,
wherein the wild-type plant LYK3 or CERK6 LysM receptor polypeptide comprises a region II characterized by the amino acids that align to residues 44-49 of SEQ ID NO: 164 and comprises a region IV characterized by the amino acids that align to residues 76-81 of SEQ ID NO: 164,
wherein the modified plant LYK3 or CERK6 LysM receptor polypeptide comprises SEQ ID NO: 87 at region II and SEQ ID NO: 129 at region IV, and
wherein the modified plant LYK3 or CERK6 LysM receptor polypeptide has altered binding kinetics for one or more Nod factors as compared to its respective wild-type plant LYK3 or CERK6 LysM receptor polypeptide.
2 . The method of claim 1 , wherein the Nod factor is a Nod factor produced by nitrogen-fixing bacteria and the nitrogen fixing bacteria is Mesorhizobium or Sinorhizobium.
3 . The method of claim 1 , wherein the modified plant LYK3 or CERK6 LysM receptor polypeptide binds one or more Nod factors with;
(a) higher selectivity and higher affinity as compared to the respective wild-type plant LYK3 or CERK6 LysM receptor polypeptide; or
(b) altered specificity as compared to the respective wild-type plant LYK3 or CERK6 LysM receptor polypeptide.
4 . The method of claim 1 , wherein the nucleic acid is modified by site-directed mutagenesis, by chemical synthesis, by genetic editing, or by genetic engineering.
5 . The method of claim 1 , wherein the nucleic acid encoding the wild-type plant LYK3 or CERK6 LysM receptor polypeptide is an endogenous plant gene in a plant cell.
6 . A genetically altered nodulating plant comprising the modified plant LYK3 or CERK6 LysM receptor polypeptide encoded by the modified nucleic acid produced by the method of claim 1 .
7 . The plant of claim 6 , wherein the nodulating plant is a leguminous plant.
8 . The genetically altered plant of claim 6 , wherein the modified nucleic acid is a transgene.
9 . The genetically altered plant of claim 6 , wherein the nucleic acid encoding the wild-type plant LYK3 or CERK6 LysM receptor polypeptide is an endogenous plant LYK3 or CERK6 LysM receptor gene.
10 . The plant of claim 7 , wherein the leguminous plant is bean, soybean, pea, chickpea, cowpea, pigeon pea, lentil, Bambara groundnut, lupin, pulses, Medicago spp., Lotus spp., forage legumes, indigo, or legume trees.
Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Nov 30, 2023
From: RADUTOIU, ELENA SIMONA; ANDERSON, KASPER RØJKJÆR; JENSEN, JENS STOUGAARD; LIRONI, DAMIANO; KRÖNAUER, CHRISTINA; LAURSEN, METTE
To: AARHUS UNIVERSITET
Reel/Frame 065723/0791 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Jun 28, 2021
From: RADUTOIU, SIMONA; ANDERSEN, KASPER RØJKJÆR; STOUGAARD, JENS; LIRONI, DAMIANO; KRÖNAUER, CHRISTINA; LAURSEN, METTE
To: AARHUS UNIVERSITET
Reel/Frame 056693/0216 →
Continuity (2)
Provisional Application
63027151
· May 19, 2020
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