Citrate synthase variant and method for producing O-acetyl-L-homoserine or L-methionine using same
The present disclosure relates to a citrate synthase variant, a microorganism comprising the variant, and a method for producing O-acetyl-L-homoserine and L-methionine using the microorganism.
1 . A citrate synthase variant having citrate synthase activity,
wherein the amino acid at position corresponding to position 415 of the polypeptide of SEQ ID NO: 1 is histidine,
the amino acid at the position corresponding to position 241 of the polypeptide of SEQ ID NO: 1 is threonine, and
the variant has an amino acid sequence at least 95% identical to SEQ ID NO: 1.
2 . The variant of claim 1 , wherein the variant comprises the amino acid sequence of SEQ ID NO: 6.
3 . The variant of claim 1 , wherein the variant comprises the amino acid sequence of SEQ ID NO: 33, in which the amino acid at position 1 of SEQ ID NO: 33 is asparagine or a serine, the amino acid at position 9 of SEQ ID NO: 33 is alanine or glutamic acid, and the amino acid at position 73 of SEQ ID NO: 33 is tyrosine or cysteine.
4 . The variant of claim 1 , wherein the variant comprises an amino acid sequence at least 95% identical to SEQ ID NO: 6.
5 . A polynucleotide encoding a citrate synthase variant having citrate synthase activity,
wherein the amino acid at position corresponding to position 415 of the polypeptide of SEQ ID NO: 1 is histidine,
the amino acid at the position corresponding to position 241 of the polypeptide of SEQ ID NO: 1 is threonine, and
the variant has an amino acid sequence at least 95% identical to SEQ ID NO: 1.
6 . A microorganism of the genus Corynebacterium , comprising
(a) a citrate synthase variant having citrate synthase activity, wherein the amino acid at the position corresponding to position 415 of the polypeptide of SEQ ID NO: 1 is histidine, the amino acid at the position corresponding to position 241 of the polypeptide of SEQ ID NO: 1 is threonine, and the variant has an amino acid sequence at least 95% identical to SEQ ID NO: 1; or
(b) a polynucleotide encoding the citrate synthase variant of (a).
7 . The microorganism of claim 6 , wherein the microorganism has an O-acetyl-L-homoserine-producing ability.
8 . The microorganism of claim 6 , wherein the microorganism is Corynebacterium glutamicum.
9 . A method for producing O-acetyl-L-homoserine or L-methionine, comprising culturing the microorganism of claim 6 in a medium.
10 . The method of claim 9 , wherein the method further comprises recovering O-acetyl-L-homoserine or L-methionine from the cultured medium or microorganism.
11 . The method of claim 9 , wherein the method for producing L-methionine further comprises converting the O-acetyl-L-homoserine into L-methionine.
12 . A composition for producing O-acetyl-L-homoserine or L-methionine, that comprises the microorganism of claim 6 and a growth medium.
13 . The variant of claim 1 , wherein the variant comprises an amino acid sequence at least 98% identical to SEQ ID NO: 1.
14 . The variant of claim 1 , wherein the variant comprises an amino acid sequence at least 99% identical to SEQ ID NO: 1.
15 . A microorganism comprising the variant of claim 1 .
16 . A microorganism comprising the variant of claim 5 .
17 . A method for producing O-acetyl-L-homoserine or L-methionine, comprising culturing the microorganism of claim 16 in a medium.
18 . The method of claim 17 , wherein the method further comprises recovering O-acetyl-L-homoserine or L-methionine from the cultured medium or microorganism.
19 . A method for producing O-acetyl-L-homoserine or L-methionine, comprising culturing the microorganism of claim 15 in a medium.
20 . The method of claim 19 , wherein the method further comprises recovering O-acetyl-L-homoserine or L-methionine from the cultured medium or microorganism.