Promoter and use thereof
The present invention relates to a novel promoter, a vector comprising the promoter, a microorganism comprising the promoter or the vector, and a method for producing a target product using the microorganism.
1. A nucleic acid molecule having a promoter activity consisting of any one of the nucleotide sequence selected from the group consisting of SEQ ID NOS: 1 to 3.
2. A gene expression cassette comprising the nucleic acid molecule of claim 1 and a target gene.
3. A recombinant vector comprising the nucleic acid molecule of claim 1 .
4. A recombinant microorganism of the genus Corynebacterium comprising the nucleic acid molecule of claim 1 .
5. The recombinant microorganism according to claim 4 , wherein the microorganism of the genus Corynebacterium is Corynebacterium glutamicum or Corynebacterium ammoniagenesis.
6. A method of producing a target product, comprising:
(a) culturing the recombinant microorganism of claim 4 in a medium; and
(b) recovering a target product from the microorganism or the medium.
7. The method according to claim 6 , wherein the target product is psicose, tagatose, or an amino acid.
8. A recombinant vector comprising the gene expression cassette of claim 2 .
9. A recombinant microorganism of the genus Corynebacterium comprising the recombinant vector of claim 3 .
10. A recombinant microorganism of the genus Corynebacterium comprising the recombinant vector of claim 8 .
11. The recombinant microorganism according to claim 9 , wherein the microorganism of the genus Corynebacterium is Corynebacterium glutamicum or Corynebacterium ammoniagenesis.
12. The recombinant microorganism according to claim 10 , wherein the microorganism of the genus Corynebacterium is Corynebacterium glutamicum or Corynebacterium ammoniagenesis.
13. A method of producing a target product, comprising:
(a) culturing the recombinant microorganism of claim 9 in a medium; and
(b) recovering a target product from the microorganism or the medium.
14. A method of producing a target product, comprising:
(a) culturing the recombinant microorganism of claim 10 in a medium; and
(b) recovering a target product from the microorganism or the medium.
15. The method according to claim 13 , wherein the target product is psicose, tagatose, or an amino acid.
16. The method according to claim 14 , wherein the target product is psicose, tagatose, or an amino acid.