Isopropylmalate synthase variant and a method of producing L-leucine using the same
The present disclosure relates to an isopropylmalate synthase variant and a method of producing L-leucine using the same.
1 . A polypeptide variant having an isopropylmalate synthase activity, comprising one or more substitutions selected from the group consisting of i) a substitution of an amino acid residue corresponding to position 138 with another amino acid residue, ii) a substitution of an amino acid residue corresponding to position 162 with another amino acid residue, iii) a substitution of an amino acid residue corresponding to position 211 with another amino acid residue, iv) a substitution of an amino acid residue corresponding to position 245 with another amino acid residue, and v) a substitution of an amino acid residue corresponding to position 588 with another amino acid residue, in the amino acid sequence of SEQ ID NO: 1.
2 . The polypeptide variant of claim 1 , wherein i) leucine, which is the amino acid residue corresponding to position 138, is substituted with glycine.
3 . The polypeptide variant of claim 1 , wherein ii) histidine, which is the amino acid residue corresponding to position 162, is substituted with glutamate.
4 . The polypeptide variant of claim 1 , wherein iii) serine, which is the amino acid residue corresponding to position 211, is substituted with leucine.
5 . The polypeptide variant of claim 1 , wherein iv) asparagine, which is the amino acid residue corresponding to position 245, is substituted with serine.
6 . The polypeptide variant of claim 1 , wherein v) isoleucine, which is the amino acid residue corresponding to position 588, is substituted with proline.
7 . The polypeptide variant of claim 1 , comprising any one or more amino acid sequences selected from the group consisting of SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, and SEQ ID NO: 14.
8 . A polynucleotide encoding the polypeptide variant of claim 1 .
9 . A vector comprising the polynucleotide of claim 8 .
10 . A microorganism of the genus Corynebacterium producing L-leucine, the microorganism comprising the polypeptide variant of claim 1 ; a polynucleotide encoding the same;
or a vector including the same.
11 . The microorganism of the genus Corynebacterium of claim 10 , wherein the microorganism of the genus Corynebacterium is Corynebacterium glutamicum.
12 . A method of producing L-leucine, the method comprising a step of culturing, in a medium, a microorganism of the genus Corynebacterium producing L-leucine, the microorganism comprising the polypeptide variant of claim 1 ; a polynucleotide encoding the same; or a vector including the same.
13 . The method of claim 12 , further comprising a step of recovering L-leucine from the medium or from the microorganism after the step of culturing.
14 . A composition for producing L-leucine, the composition comprising a microorganism of genus Corynebacterium producing L-leucine, the microorganism comprising the polypeptide variant of claim 1 , a polynucleotide encoding the same, or a vector including the same; or a medium in which the microorganism is cultured.
15 . The polypeptide variant according to claim 1 , comprising at least 80% sequence identity to SEQ ID NO: 1.