Method of producing sulfur-containing amino acid or derivative thereof
A method of producing sulfur-containing amino acids or derivatives of the sulfur-containing amino acids.
1. A method of producing a sulfur-containing amino acid or a derivative of the sulfur-containing amino acid, the method comprising culturing a genetically modified microorganism in a culture medium including thiosulfate, and recovering the sulfur-containing amino acid or the derivative of the sulfur-containing amino acid from the microorganism or the culture medium,
wherein the microorganism comprises a genetic modification to increase an expression level of a protein encoded by ssuD gene compared to a non-modified microorganism,
wherein the production of the sulfur-containing amino acid by the microorganism comprising the genetic modification is increased compared to the non-modified microorganism;
wherein the protein encoded by the ssuD gene comprises an amino acid sequence having at least 80% sequence identity to SEQ ID NO: 43.
2. The method of claim 1 , wherein the protein encoded by ssuD gene has thiosulfate reductase activity.
3. The method of claim 1 , wherein the protein encoded by ssuD gene comprises an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 43.
4. The method of claim 1 , wherein the microorganism is a microorganism belonging to the genus Corynebacterium or the genus Escherichia sp.
5. The method of claim 4 , wherein the microorganism is Corynebacterium glutamicum, Corynebacterium callunae, Corynebacterium deserti, Corynebacterium crenatum , or Escherichia coli.
6. The method of claim 1 , wherein genetic modification to increase the activity of the protein is achieved by i) increasing a copy number of a polynucleotide encoding the protein in a cell, ii) replacing an expression regulatory region of a polynucleotide encoding the protein with a sequence with stronger activity, iii) modifying an initiation codon or 5′-UTR of a polynucleotide encoding the protein, iv) modifying a nucleotide sequence on a chromosome to enhance the activity of the protein, v) introducing a foreign polynucleotide expressing the activity of the protein or a codon optimized variant polynucleotide of the polynucleotide encoding the protein, or vi) a combination thereof.
7. The method of claim 1 , wherein the sulfur-containing amino acid or the derivative of the sulfur-containing amino acid comprises at least one selected from the group consisting of methionine, cysteine, cystine, lanthionine, homocysteine, homocystine, homolanthionine, and taurine.