IP Library › Granted Patent US 12,653,847
Granted Patent B2
US 12,653,847 · App. 18/508,430 · Granted Jun 16, 2026

Patent

Inventors: John Deaton (Kennesaw, GA); Ana Maria Cuentas (Woodstock, GA)
Assignee: Deerland Enzymes, Inc.
A61K35/742C12R2001/125
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Quick Facts
Patent No.
US 12,653,847
App. No.
18/508,430
Granted
Jun 16, 2026
Kind
B2
Abstract

Methods of using Bacillus subtilis probiotic supplementation to promote lower circulating TNF-α in resistance trained males are presented. Methods of administering Bacillus subtilis probiotic supplementation to an athletic population in conjunction with a sound nutrition and training regimen are presented herein. College athletes typically undergo periods of elevated stress both physically and mentally which may negatively affect recovery and adaptation. 12-weeks of probiotic supplementation resulted in attenuated circulating TNF-α concentrations in college athletes following offseason training.

Claims (17)

1 . A method of reducing tumor necrosis factor-alpha (TNF-α) in human serum, comprising the steps of:

(a) administering orally to a human a composition comprising an isolated strain of Bacillus subtilis subspecies inaquosorum having accession number NRRL B-67989 in a dose of from about 1·10 8 colony forming units (CFU) per day to about 1·10 11 CFU per day for about 12 weeks; and

(b) submitting the human to a resistance training program throughout the 12 weeks.

2 . The method of claim 1 , wherein the resistance program includes workouts 2-3 times per week.

3 . The method of claim 1 , further comprising the steps of measuring TNF-α in blood plasma of the human both before and after steps (a) and (b).

4 . The method of claim 1 , wherein the composition comprises the isolated strain of Bacillus subtilis in a dose of from about 1·10 9 CFU per day to about 1·10 10 CFU per day.

5 . The method of claim 4 , wherein the TNF-α level is reduced by about 0.2 pg/mL in serum.

6 . A method for treating or reducing inflammation in a human, comprising the steps of:

(a) administering orally to the human in need of such treatment a composition comprising an isolated strain of Bacillus subtilis subspecies inaquosorum having accession number NRRL B-67989 in a dose of from about 1·10 8 CFU per day to about 1·10 11 CFU per day for about 12 weeks, and

(b) measuring TNF-α in blood plasma of the human,

wherein the TNF-α level is reduced in serum.

7 . The method of claim 6 , further comprising the step of submitting the human to a resistance training program throughout the 12 weeks of step (a).

8 . The method of claim 6 , wherein the composition comprises the isolated strain of Bacillus subtilis in a dose of from about 1·10 9 CFU per day to about 1·10 10 CFU per day.

9 . The method of claim 7 , wherein the composition comprises the isolated strain of Bacillus subtilis in a dose of from about 1·10 9 CFU per day to about 1·10 10 CFU per day.

10 . The method of claim 9 , wherein the resistance program includes workouts 2-3 times per week.

11 . The method of claim 10 , further comprising the step of measuring TNF-α in blood plasma of the human before step (a).

12 . The method of claim 11 , wherein the TNF-α level is reduced by about 0.2 pg/mL in serum.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jan 28, 2025
From: DEATON, JOHN; CUENTAS, ANA MARIA
To: DEERLAND ENZYMES, INC.
Reel/Frame 070031/0961 →
Continuity (3)
Continuation In Part 16593469 · Oct 4, 2019
Provisional Application 62741300 · Oct 4, 2018
Related Publication 20240075078A1 · Mar 7, 2024
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