Anesthetic method of spotted seabass with plant essential oils
An anesthetic method of the spotted seabass with a plant essential oil includes the following steps. (1) Spotted seabass is acclimated in a water tank for 24 h before anesthesia. The metabolic wastes of the spotted seabass were discharged during acclimatization period. (2) Cinnamomum camphora essential oil, ethanol, and Tween-80 are mixed and dissolved to obtain an emulsified essential oil. (3) The emulsified essential oil is respectively added into a plurality of aquariums. The spotted seabass is transferred into the plurality of aquariums. Concentrations of Cinnamomum camphora essential oils in the plurality of aquariums and anesthesia time of the spotted seabass are recorded. (4) The spotted seabass are captured and placed into an aquarium containing clean water for recovery when the spotted seabass enter the deep anesthesia period. The resuscitation time is recorded when the spotted seabass have no flopping, and swim normally and is active.
1 . A method of anesthetizing a spotted seabass with a Cinnamomum camphora essential oil, consisting essentially of:
a) acclimating the spotted seabass in a water tank for 24 hours before performing anesthesia, wherein the water quality parameters are maintained within following ranges: dissolved oxygen at 6.0 mg/L, temperature at 22±1° C., salinity of 16%, and pH at 7.5-8.0;
b) discharging the metabolic wastes of the spotted seabass during an acclimatization period;
c) emulsifying and dissolving Cinnamomum camphora essential oil, ethanol, and Polysorbate 80 to obtain an emulsified Cinnamomum camphora essential oil;
d) adding the emulsified Cinnamomum camphora essential oil into seven different aquariums at concentrations of 10 mg/l, 20 mg/l, 30 mg/l, 50 mg/l, 100 mg/l, 150 mg/l, and 200 mg/l, respectively,
e) transferring the spotted seabass to the seven different aquariums with the emulsified Cinnamomum camphora essential oils of the different concentrations, respectively;
f) recording the concentrations of the Cinnamomum camphora essential oils in the seven different aquariums of spotted seabass and the anesthesia time of the spotted seabass;
g) extracting the spotted seabasses once deep anesthesia is achieved; and
h) recording the resuscitation time of the spotted seabasses when the spotted seabasses restore their swimming behavior.