IP Library › Granted Patent US 12,655,130
Granted Patent B2
US 12,655,130 · App. 17/920,544 · Granted Jun 16, 2026

5-membered heteroaryl carboxamide compounds for treatment of HBV

Inventors: Thilo Heckrodt (South San Francisco, CA); Michael Walker (South San Francisco, CA); Min Zhong (South San Francisco, CA)
Assignee: ASSEMBLY BIOSCIENCES, INC.
C07D403/08A61P31/20C07D233/90C07D263/34C07D277/56C07D401/08C07D471/04C07D487/04C07D498/04C07B2200/05
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Quick Facts
Patent No.
US 12,655,130
App. No.
17/920,544
Granted
Jun 16, 2026
Kind
B2
Abstract

The present disclosure relates to 5-membered heteroaryl carboxamide compounds, which may disrupt Hepatitis B (HBV) core protein assembly. The present disclosure further relates to pharmaceutical compositions comprising 5-membered heteroaryl carboxamide compounds which may disrupt HBV core protein assembly. The present disclosure further relates to uses of 5-membered heteroaryl carboxamide compounds and pharmaceutical compositions thereof in methods of treating Hepatitis B (HBV) infection.

Claims (279)

1 . A compound of Formula I

or a pharmaceutically acceptable salt thereof, wherein:

L is C 1-4 alkylene or haloC 1-4 alkylene;

L 1 is a bond, C 1-6 alkylene, O, NR c , C(O), C(O)O, C(O)NR c , S(O) t or S(O) t NR c ;

X 1 is NR x1 ;

X 3 is CR 4 R 8 ;

R a , R b and R c are independently selected for each occurrence from the group consisting of hydrogen, C 1-6 alkyl, haloC 1-6 alkyl and C 3-6 monocycloalkyl;

R d is hydrogen, OH, C 1-6 alkyl or C 1-6 alkoxy;

R x1 is hydrogen, C 1-4 alkyl, C 1-4 alkenyl, C 1-4 alkynyl, haloC 1-4 alkyl, or C 3-6 monocycloalkyl; or R x1 and R 2 together form a —CH 2 CH 2 CH 2 —, —CH 2 CH 2 CH 2 CH 2 —, —CH 2 CH 2 O—, —CH 2 OCH 2 —, —CH 2 CH 2 CH 2 O— —CH 2 CH 2 OCH 2 —, —CH 2 CH 2 —NH— —CH 2 NHCH 2 —, —CH 2 CH 2 CH 2 NH— or —CH 2 CH 2 NHCH 2 — group;

R 0a is independently selected for each occurrence from the group consisting of hydrogen, halogen, OH, CN, NO 2 , R a R b N—, C 1-4 alkyl and haloC 1-4 alkyl;

R 6a are independently hydrogen or C 1-4 alkyl;

R 0 , R 6 are independently selected for each occurrence from the group consisting of hydrogen, halogen, OH, CN, NO 2 , oxo, R d N═, hydrazino, formyl, azido, silyl, siloxy, HOC(O)—, R a R b N—, R a R b NS(O) t —, C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, haloC 1-6 alkyl, hydroxyC 1-6 alkyl-, R a R b NC 1-6 alkyl-, HOC(O)C 1-6 alkyl-, R a R b NC 1-6 alkylNR c —, C 1-6 alkylNR a C 1-6 alkylNR c —, C 1-6 alkoxy, haloC 1-6 alkoxy, hydroxyC 1-6 alkoxy-, R a R b NC 1-6 alkoxy-, C 1-6 alkoxyC 1-6 alkyl-, haloC 1-6 alkoxyC 1-6 alkyl-, R a R b NC(O)—, C 1-6 alkylC(O)—, C 1-6 alkoxyC(O)—, C 1-6 alkylC(O)O—, C 1-6 alkylS(O) q —, C 1-6 alkylS(O) t NR c —, C 1-6 alkylS(O) t C 1-6 alkyl-, C 1-6 alkylS(O) t NR a C 1-6 alkyl-, C 3-6 cycloalkylS(O) t C 1-6 alkyl-, C 1-6 alkylC(O)C 1-6 alkyl-, and C 1-6 alkylC(O)OC 1-6 alkyl-;

each R 11 independently is hydrogen, OH, CN, NO 2 , C 1-6 alkyl, haloC 1-6 alkyl, HOC(O)—, HOC(O)C 1-6 alkyl, R a R b N—, C 2-6 alkenyl, C 2-6 alkynyl, C 1-6 alkoxy, haloC 1-6 alkoxy, or C 1-6 alkoxy;

R 1 is a phenyl or 5-6 membered monocyclic heteroaryl, wherein the phenyl or 5-6 membered monocyclic heteroaryl is optionally substituted with one, two, or three independently selected R 11 groups;

R 2 is selected from the group consisting of hydrogen, halo, CN, OH, R a R b N, C 1-4 alkyl, haloC 1-4 alkyl, C 3-5 monocycloalkyl, C 1-4 alkoxy, and haloC 1-4 alkoxy;

R 8 is selected from the group consisting of hydrogen, halo, CN, OH, R a R b N, C 1-4 alkyl, haloC 1-4 alkyl, C 3-5 monocycloalkyl, C 1-6 alkoxy, and haloC 1-4 alkoxy;

R 3 is

R 4 is R 5 -L 1 -, R 6 or R 9 ;

R 5 is

R 9 is R 14 S(O) q -L-, R 14 S(O) q NH-L-, or R 14 C(O)NH-L-;

R 14 is R a R b N—, C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, C 1-6 haloalkyl, C 1-6 alkoxy, C 1-6 haloalkyl, C 1-6 haloalkoxy, or R 5 -L 1 -;

q, r, t, and w are independently selected for each occurrence from 0, 1 and 2.

2 . The compound of claim 1 , or a pharmaceutically acceptable salt thereof, wherein R x1 is hydrogen or methyl.

3 . The compound of claim 2 , or a pharmaceutically acceptable salt thereof, wherein R x1 is methyl.

4 . The compound according to claim 1 , or a pharmaceutically acceptable salt thereof, wherein r is 0.

5 . The compound according to claim 1 , or a pharmaceutically acceptable salt thereof, wherein R 2 is hydrogen.

6 . The compound according to claim 1 , or a pharmaceutically acceptable salt thereof, wherein: R 1 is

R 11 is independently selected for each occurrence from the group consisting of halogen, CN, C 1-6 alkyl and haloC 1-6 alkyl; and z1 is 0, 1,2 or 3.

7 . The compound of claim 6 , or a pharmaceutically acceptable salt thereof, wherein for each occurrence R 11 is independently selected from the group consisting of CN, F, Cl, Br and I.

8 . The compound of claim 7 , or a pharmaceutically acceptable salt thereof, wherein R 1 is

9 . The compound of claim 7 , or a pharmaceutically acceptable salt thereof, wherein R 1 is

10 . The compound according to claim 1 , or a pharmaceutically acceptable salt thereof, wherein R 3 is

11 . The compound according to claim 1 , or a pharmaceutically acceptable salt thereof, wherein R 4 is R 6 .

12 . The compound according to claim 1 , or a pharmaceutically acceptable salt thereof, wherein R 4 is R 5 -L 1 .

13 . The compound of claim 12 , or a pharmaceutically acceptable salt thereof, wherein L 1 is a bond.

14 . The compound according to claim 1 , or a pharmaceutically acceptable salt thereof, wherein R 4 is R 9 .

15 . The compound according to claim 1 , or a pharmaceutically acceptable salt thereof, wherein R 8 is hydrogen, OH or C 1-6 alkoxy.

16 . The compound of claim 15 , or a pharmaceutically acceptable salt thereof, wherein R 8 is OH.

17 . A pharmaceutical composition comprising the compound according to claim 1 , or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable excipient.

18 . A method of treating Hepatitis B (HBV) infection in a subject in need thereof, the method comprising: administering to the subject a therapeutically effective amount of a compound according to claim 1 , or a pharmaceutically acceptable salt thereof.

19 . A method of treating Hepatitis B (HBV) infection in a subject in need thereof, the method comprising: administering to the subject a therapeutically effective amount of pharmaceutical composition of claim 17 .

20 . A compound, or a pharmaceutically acceptable salt thereof, wherein the compound is selected from:

Example

No.

Name/Structure

Example  1

Example  2

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Example  67

Example

N-(3-Chloro-4-fluorophenyl)-4-(5-hydroxy-5-

 68

(3-((1-hydroxypropan-2-yl)oxy)-1-methyl-1H-

pyrazol-5-yl)octahydropentalen-2-y1)-1-

methyl-1H-imidazole-5-carboxamide,

Example  70

Example  71

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Example 223

21 . A compound

or a pharmaceutically acceptable salt thereof.

22 . A compound

or a pharmaceutically acceptable salt thereof.

23 . A diastereomer of a compound

or a pharmaceutically acceptable salt thereof.

24 . A pharmaceutical composition comprising the compound according to claim 21 , or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable excipient.

25 . A method of treating Hepatitis B (HBV) infection in a subject in need thereof, the method comprising: administering to the subject a therapeutically effective amount of a compound according to claim 21 , or a pharmaceutically acceptable salt thereof.

26 . A method of treating HBV infection in a subject in need thereof, the method comprising: administering to the subject a therapeutically effective amount of pharmaceutical composition of claim 24 .

27 . A pharmaceutical composition comprising the compound according to claim 22 , or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable excipient.

28 . A method of treating HBV infection in a subject in need thereof, the method comprising: administering to the subject a therapeutically effective amount of a compound according to claim 22 , or a pharmaceutically acceptable salt thereof.

29 . A method of treating HBV infection in a subject in need thereof, the method comprising: administering to the subject a therapeutically effective amount of pharmaceutical composition of claim 27 .

30 . A pharmaceutical composition comprising the compound according to claim 23 , or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable excipient.

31 . A method of treating HBV infection in a subject in need thereof, the method comprising: administering to the subject a therapeutically effective amount of a compound according to claim 23 , or a pharmaceutically acceptable salt thereof.

32 . A method of treating HBV infection in a subject in need thereof, the method comprising: administering to the subject a therapeutically effective amount of pharmaceutical composition of claim 30 .

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded May 1, 2026
From: HECKRODT, THILO; WALKER, MICHAEL; ZHONG, MIN
To: ASSEMBLY BIOSCIENCES, INC.
Reel/Frame 074542/0169 →
Continuity (2)
Provisional Application 63014001 · Apr 22, 2020
Related Publication 20230183213A1 · Jun 15, 2023
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