Promoter and method for producing desired substance using same
The present application relates to a novel promoter and a method for producing a desired substance using the same.
1 . A polynucleotide comprising a nucleotide sequence represented by General Formula 1 below:
X-Y-Z [General Formula 1]
wherein,
X is CN 1 GN 2 ,
Y is CTAATTN 3 , and
Z is CATGTGTGTGGTATAAT,
in which N 1 of X is cytosine (C) or guanine (G), N 2 of X is adenine (A) or thymine (T), and N 3 of Y is adenine (A) or guanine (G).
2 . The polynucleotide of claim 1 , wherein X is any one of SEQ ID NOS: 8 to 11, and Y is SEQ ID NO: 6 or 7.
3 . The polynucleotide of claim 1 , wherein the polynucleotide comprises any one polynucleotide sequence selected from SEQ ID NOS: 13 to 20.
4 . A promoter comprising the polynucleotide of claim 1 .
5 . A vector comprising the promoter of claim 4 and a gene encoding a target protein.
6 . The vector of claim 5 , wherein the target protein is acetohydroxy acid isomeroreductase.
7 . A microorganism of the genus Corynebacterium , comprising the polynucleotide of claim 1 .
8 . The microorganism of claim 7 , wherein the microorganism of the genus Corynebacterium is Corynebacterium glutamicum.
9 . A method for producing a target substance, the method comprising:
culturing the microorganism of the genus Corynebacterium of claim 7 in a medium; and
recovering the target substance in the medium.
10 . The method of claim 9 , wherein the target substance is an amino acid.
11 . The method of claim 10 , wherein the amino acid is an L-branched-chain amino acid.