Cell culture device
A cell culture device is provided, which comprises a cavity and a base layer, wherein the base layer is a type of plastic thin film and has a thickness of 1 μm to 100 μm; a second-moment of inertia lower than 6×10 6 μm 4 ; and a resultant flexural rigidity of 1×10 −6 Pa·m 4 to 0.02 Pa·m 4 . Accordingly, the base layer can produce an out-of-plane strain, and bending deformation can occur. Therefore, a growth space close to in vivo environment is provided by the base layer for the cells when the cells attach to the base layer, thereby promoting the growth and maturation of the cells and tissues.
1 . A cell culture device, comprising:
a cavity including an opening and a bottom;
a base layer disposed at the bottom of the cavity and closing the bottom, wherein a thickness of the base layer is between 1 μm to 100 μm, a second-moment of inertia of the base layer is lower than 6×10 6 μm 4 , and a resultant flexural rigidity of the base layer is between is 1×10 −6 Pa·m 4 to 0.02 Pa·m 4 ; and
a hollow spacer disposed under the base layer and contacting with an edge of the base layer;
wherein the hollow spacer is configured to prevent the base layer from contacting any surface thereunder and allows the base layer to generate out-of-plane strain during a cell culture process;
wherein the base layer is made of a plastic film at least one selected from the group consisting of polymethyl methacrylate, polypropylene, polystyrene, polyethylene, polyethylene terephthalate, polyvinyl chloride, polydimethylsiloxane, polyurethane, polyacrylamide, and mixtures thereof;
wherein the base layer further includes a plurality of microgrooves, wherein each one of the plurality of microgrooves are arranged adjacent and parallel to each other.
2 . The cell culture device as claimed in claim 1 , wherein a width of each of the microgrooves is 5 μm to 50 μm, and a gap between the adjacent microgrooves is 5 μm to 50 μm.
3 . The cell culture device as claimed in claim 1 , wherein a depth of each of the microgrooves is between 1 μm to 10 μm.
4 . The cell culture device as claimed in claim 1 , further comprising a coating layer formed on the base layer, wherein the coating is at least one selected from the group consisting of matrix hydrogel, collagen, fibronectin, laminin, gelatin, and mixtures thereof.
5 . A cell culture multi-well plate, comprising:
a main body including an accommodating space and a first surface; and
a plurality of the cell culture device claimed in claim 1 , each of the plurality of cell culture devices is disposed in the accommodating space;
wherein the opening of the cavity of each of the plurality of cell culture devices is formed on the first surface.