Granted Patent
B2
US 12,697,419 · App. 18/390,157 · Granted Aug 4, 2026
Anti-adhesive barrier membrane using alginate and hyaluronic acid for biomedical applications
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Abstract
A non-synthetic, hydrophilic, biodegradable, biocompatible polysaccharide based non-toxic anti-adhesion hydrogel barrier is disclosed herein. The barrier of the present invention is formed by constructing a unique interpenetrating, crosslinked network with a unique porosity. Furthermore, the barrier of the present invention is comprised of tunable biopolymers for controllable mechanical robustness and degradation. The barrier of the present invention effectively reduces unwanted adhesions using non-synthetic components.
Claims (22)
1 . An apparatus comprising:
a wrap comprising first and second layers;
wherein the first layer includes a desiccated, translucent, amorphous hydrogel film, the hydrogel film comprising hyaluronic acid and sodium alginate;
wherein the second layer includes collagen;
wherein the first layer is fused to the second layer;
wherein the first layer has at least 1% hyaluronic acid by dry weight.
2 . The apparatus of claim 1 , wherein the hyaluronic acid is uncrosslinked.
3 . The apparatus of claim 2 , wherein the sodium alginate in the first layer is uncross-linked.
4 . The apparatus of claim 3 , wherein the first layer includes no more than 33% hyaluronic acid by dry weight.
5 . The apparatus of claim 4 , wherein the first and second layers are both flat and planar.
6 . The apparatus of claim 4 , wherein the hydrogel film consists essentially of the hyaluronic acid and the sodium alginate.
7 . The apparatus of claim 4 , wherein the hydrogel film is unpatterned on at least one surface of the hydrogel film.
8 . The apparatus of claim 7 , wherein the first layer has a degree of bioabsorbability based on a ratio of the hyaluronic acid to the sodium alginate.
9 . The apparatus of claim 8 , wherein the hydrogel film is flat and includes a width, a length, and a thickness that is less than the width and the length.
10 . The apparatus of claim 8 , wherein the hydrogel film includes an anionic polymer.
11 . The apparatus of claim 10 , wherein the first layer is negatively charged.
12 . The apparatus of claim 11 , wherein the hydrogel film is non-synthetic.
13 . The apparatus of claim 12 , wherein the hydrogel film is a cast film.
14 . The apparatus of claim 12 , wherein the first layer is hydrophilic.
15 . The apparatus of claim 14 , wherein the first layer is anti-adhesive and non-attractive to cells.
16 . The apparatus of claim 15 , wherein the second layer is adhesive to cells.
17 . The apparatus of claim 16 , wherein the first layer is non-cytotoxic.
Continuity (8)
Provisional Application
61391299
· Oct 8, 2010
Related Publication
20240226389A1
· Jul 11, 2024
References Cited (336)
US 4141973A
· Balazs
· 1979
[cited by applicant]
US 4196070A
· Chao et al.
· 1980
[cited by applicant]
US 4818542A
· DeLuca et al.
· 1989
[cited by applicant]
US 4937270A
· Hamilton et al.
· 1990
[cited by applicant]
US 5017229A
· Burns et al.
· 1991
[cited by applicant]
US 5204331A
· Nishi
· 1993
[cited by applicant]
US 5266326A
· Barry et al.
· 1993
[cited by applicant]
US 5395383A
· Adams et al.
· 1995
[cited by applicant]
US 5415631A
· Churinetz
· 1995
[cited by applicant]
US 5531716A
· Luzio et al.
· 1996
[cited by applicant]
US 5531735A
· Thompson
· 1996
[cited by applicant]
US 5532221A
· Huang et al.
· 1996
[cited by applicant]
US 5563186A
· Thompson
· 1996
[cited by applicant]
US 5622707A
· Dorigatti et al.
· 1997
[cited by applicant]
US 5688775A
· Renn et al.
· 1997
[cited by applicant]
US 5714166A
· Tomalia
· 1998
[cited by applicant]
US 5750585A
· Park et al.
· 1998
[cited by applicant]
US 5760200A
· Miller et al.
· 1998
[cited by applicant]
US 5795584A
· Totakura
· 1998
[cited by applicant]
US 5863551A
· Woerly
· 1999
[cited by applicant]
US 5866554A
· Shalaby et al.
· 1999
[cited by applicant]
US 5874100A
· Mahoney et al.
· 1999
[cited by applicant]
US 5919442A
· Yin et al.
· 1999
[cited by applicant]
US 5925009A
· Mahoney et al.
· 1999
[cited by applicant]
US 5939323A
· Valentini et al.
· 1999
[cited by applicant]
US 5981821A
· Barikosky
· 1999
[cited by applicant]
US 5981825A
· Brekke
· 1999
[cited by applicant]
US 5984948A
· Hassan
· 1999
[cited by applicant]
US 5993661A
· Ruckenstein et al.
· 1999
[cited by applicant]
US 6007833A
· Chudzik
· 1999
[cited by applicant]
US 6030958A
· Burns et al.
· 2000
[cited by applicant]
US 6060534A
· Ronan et al.
· 2000
[cited by applicant]
US 6083930A
· Roufa et al.
· 2000
[cited by applicant]
US 6096018A
· Luzio et al.
· 2000
[cited by applicant]
US 6124273A
· Drohan et al.
· 2000
[cited by applicant]
US 6133325A
· Schwartz et al.
· 2000
[cited by applicant]
US 6150581A
· Jiang et al.
· 2000
[cited by applicant]
US 6156572A
· Bellamkonda
· 2000
[cited by applicant]
US 6165201A
· Sawhney et al.
· 2000
[cited by applicant]
US 6174999B1
· Miller et al.
· 2001
[cited by applicant]
US 6184266B1
· Ronan et al.
· 2001
[cited by applicant]
US 6235726B1
· Burns
· 2001
[cited by applicant]
US 6271278B1
· Park et al.
· 2001
[cited by applicant]
US 6281341B1
· Mares-Guia et al.
· 2001
[cited by applicant]
US 6294202B1
· Burns et al.
· 2001
[cited by applicant]
US 6334968B1
· Shapiro et al.
· 2002
[cited by applicant]
US 6368356B1
· Zhong et al.
· 2002
[cited by applicant]
US 6372244B1
· Antanavich
· 2002
[cited by applicant]
US 6387878B1
· Ronan
· 2002
[cited by applicant]
US 6387978B2
· Ronan et al.
· 2002
[cited by applicant]
US 6410044B1
· Chudzik
· 2002
[cited by applicant]
US 6425918B1
· Shapiro et al.
· 2002
[cited by applicant]
US 6500777B1
· Wiseman et al.
· 2002
[cited by applicant]
US 6511650B1
· Eiselt
· 2003
[cited by applicant]
US 6521223B1
· Calias et al.
· 2003
[cited by applicant]
US 6548081B2
· Sadozai et al.
· 2003
[cited by applicant]
US 6565878B2
· Schoenfeldt et al.
· 2003
[cited by applicant]
US 6566345B2
· Miller et al.
· 2003
[cited by applicant]
US 6599526B2
· Dimitrijevich
· 2003
[cited by applicant]
US 6600011B2
· McDonnell
· 2003
[cited by applicant]
US 6608117B1
· Gvozdic
· 2003
[cited by applicant]
US 6610669B1
· Calias et al.
· 2003
[cited by applicant]
US 6630167B2
· Zhang
· 2003
[cited by applicant]
US 6630457B1
· Aeschlimann et al.
· 2003
[cited by applicant]
US 6638917B1
· Li et al.
· 2003
[cited by applicant]
US 6642363B1
· Mooney et al.
· 2003
[cited by applicant]
US 6653240B2
· Crawford
· 2003
[cited by applicant]
US 6653420B2
· Domschke
· 2003
[cited by applicant]
US 6656974B1
· Renn et al.
· 2003
[cited by applicant]
US 6693089B1
· Li et al.
· 2004
[cited by applicant]
US 6703041B2
· Burns et al.
· 2004
[cited by applicant]
US 6723709B1
· Pressato et al.
· 2004
[cited by applicant]
US 6750262B1
· Hahnle
· 2004
[cited by applicant]
US 6767928B1
· Murphy et al.
· 2004
[cited by applicant]
US 6793675B2
· Shapiro
· 2004
[cited by applicant]
US 6818018B1
· Sawhney
· 2004
[cited by applicant]
US 6841153B1
· Chegini et al.
· 2005
[cited by applicant]
US 6869938B1
· Schwartz et al.
· 2005
[cited by applicant]
US 6897271B1
· Domschke
· 2005
[cited by applicant]
US 6913765B2
· Li et al.
· 2005
[cited by applicant]
US 6924370B2
· Chudzik
· 2005
[cited by applicant]
US 6939562B2
· Spiro
· 2005
[cited by applicant]
US 6943154B2
· Miller et al.
· 2005
[cited by applicant]
US 6960617B2
· Omidian
· 2005
[cited by applicant]
US 6977231B1
· Matsuda
· 2005
[cited by applicant]
US 6991652B2
· Burg
· 2006
[cited by applicant]
US 7022313B2
· O'Connor
· 2006
[cited by applicant]
US 7060287B1
· Hubbard et al.
· 2006
[cited by applicant]
US 7083697B2
· Dao et al.
· 2006
[cited by applicant]
US 7201917B2
· Malaviya
· 2007
[cited by applicant]
US 7235295B2
· Laurencin et al.
· 2007
[cited by applicant]
US 7252832B1
· Stone et al.
· 2007
[cited by applicant]
US 7265098B2
· Miller et al.
· 2007
[cited by applicant]
US 7347850B2
· Sawhney
· 2008
[cited by applicant]
US 7347988B2
· Hu et al.
· 2008
[cited by applicant]
US 7459021B2
· Bukshpan
· 2008
[cited by applicant]
US 7504286B2
· Cho
· 2009
[cited by applicant]
US 7553903B2
· Riegel
· 2009
[cited by applicant]
US 7572894B2
· Jin et al.
· 2009
[cited by applicant]
US 7592418B2
· Pathak et al.
· 2009
[cited by applicant]
US 7629388B2
· Mikos et al.
· 2009
[cited by applicant]
US 7682540B2
· Boyan et al.
· 2010
[cited by applicant]
US 7741476B2
· Lebreton
· 2010
[cited by applicant]
US 7758654B2
· Hoganson
· 2010
[cited by applicant]
US 7833284B2
· Lieberman
· 2010
[cited by applicant]
US 7919542B2
· Hudgins
· 2011
[cited by applicant]
US 7968110B2
· Hubbard
· 2011
[cited by applicant]
US 7988992B2
· Omidian
· 2011
[cited by applicant]
US 7989505B2
· Hu et al.
· 2011
[cited by applicant]
US 7998204B2
· Stone et al.
· 2011
[cited by applicant]
US 7998380B2
· Turng
· 2011
[cited by applicant]
US 8025901B2
· Kao
· 2011
[cited by applicant]
US 8075908B2
· Delaney
· 2011
[cited by applicant]
US 8097273B2
· Fukuhira et al.
· 2012
[cited by applicant]
US 8110242B2
· Hawkins
· 2012
[cited by applicant]
US 8133840B2
· Mika et al.
· 2012
[cited by applicant]
US 8277831B2
· Young et al.
· 2012
[cited by applicant]
US 8323675B2
· Greenawalt
· 2012
[cited by applicant]
US 8324184B2
· Prestwich et al.
· 2012
[cited by applicant]
US 8455001B2
· Ito et al.
· 2013
[cited by applicant]
US 8460695B2
· Greenawalt
· 2013
[cited by applicant]
US 8551136B2
· Lu
· 2013
[cited by applicant]
US 8668863B2
· Zawko
· 2014
[cited by applicant]
US 8802115B2
· Altschuler et al.
· 2014
[cited by applicant]
US 8809301B2
· Athanasiadis et al.
· 2014
[cited by applicant]
US 8927521B2
· Jackson
· 2015
[cited by applicant]
US 9034357B2
· Stopek
· 2015
[cited by applicant]
US 9095558B2
· Mayes
· 2015
[cited by examiner]
US 9421221B2
· McKay et al.
· 2016
[cited by applicant]
US 9656001B2
· Mayes
· 2017
[cited by examiner]
US 9770539B2
· Parakka et al.
· 2017
[cited by applicant]
US 9987130B2
· Weber
· 2018
[cited by applicant]
US 20020131933A1
· Delmotte
· 2002
[cited by applicant]
US 20040091605A1
· Bayer et al.
· 2004
[cited by applicant]
US 20040138329A1
· Hubbell et al.
· 2004
[cited by applicant]
US 20040241436A1
· Hsieh et al.
· 2004
[cited by applicant]
US 20050107868A1
· Nakayama
· 2005
[cited by applicant]
US 20070031498A1
· Zhong et al.
· 2007
[cited by applicant]
US 20070051630A1
· Larsson et al.
· 2007
[cited by applicant]
US 20070202084A1
· Sadozai et al.
· 2007
[cited by applicant]
US 20080069857A1
· Yeo
· 2008
[cited by applicant]
US 20080182012A1
· Fisher et al.
· 2008
[cited by applicant]
US 20080254091A1
· Lee et al.
· 2008
[cited by applicant]
US 20080264793A1
· Vigh et al.
· 2008
[cited by applicant]
US 20080292664A1
· Giammona et al.
· 2008
[cited by applicant]
US 20090062233A1
· Ji et al.
· 2009
[cited by applicant]
US 20090170973A1
· Mattiasson et al.
· 2009
[cited by applicant]
US 20090294049A1
· Udipi et al.
· 2009
[cited by applicant]
US 20100055158A1
· Vitaris
· 2010
[cited by applicant]
US 20100062232A1
· Schauer et al.
· 2010
[cited by applicant]
US 20100114313A1
· Lack
· 2010
[cited by applicant]
US 20100121261A1
· Kablik et al.
· 2010
[cited by applicant]
US 20100217403A1
· Champion et al.
· 2010
[cited by applicant]
US 20100273667A1
· Kotov
· 2010
[cited by applicant]
US 20120039959A1
· Tessmar et al.
· 2012
[cited by applicant]
US 20120189760A1
· Steel
· 2012
[cited by applicant]
US 20120244107A1
· Heckroth et al.
· 2012
[cited by applicant]
US 20130034592A1
· Yamamoto et al.
· 2013
[cited by applicant]
US 20130052236A1
· Tessmar et al.
· 2013
[cited by applicant]
US 20130195789A1
· Lu
· 2013
[cited by applicant]
US 20130211320A1
· Alkhamesi et al.
· 2013
[cited by applicant]
US 20130252921A1
· Lee et al.
· 2013
[cited by applicant]
US 20130316007A1
· Ma et al.
· 2013
[cited by applicant]
US 20140256831A1
· Ito et al.
· 2014
[cited by applicant]
US 20150010636A1
· Delaney
· 2015
[cited by applicant]
US 20210269638A1
· Kim et al.
· 2021
[cited by applicant]
US 20230095832A1
· Ng
· 2023
[cited by applicant]
US 20230101687A1
· Traverso et al.
· 2023
[cited by applicant]
CN 102665604B
· 2012
[cited by applicant]
CN 108969392A
· 2018
[cited by applicant]
CN 109294002B
· 2021
[cited by applicant]
CN 113813448B
· 2022
[cited by applicant]
CN 116019973A
· 2023
[cited by applicant]
CN 114984300B
· 2023
[cited by applicant]
EP 1105094B1
· 1999
[cited by applicant]
EP 1778144B1
· 2005
[cited by applicant]
EP 1806367A3
· 2007
[cited by applicant]
IN 202011004849A
· 2021
[cited by applicant]
JP H04235124A
· 1992
[cited by applicant]
JP H06100468A
· 1994
[cited by applicant]
JP 2000271207A
· 2000
[cited by applicant]
JP 2001212224A
· 2001
[cited by applicant]
JP 2002539855A
· 2002
[cited by applicant]
JP 2003062057A
· 2003
[cited by applicant]
JP 2004507472A
· 2004
[cited by applicant]
JP 2004507472
· 2004
[cited by examiner]
JP 2006006448A
· 2006
[cited by applicant]
JP 3805654B2
· 2006
[cited by applicant]
JP 3796165B2
· 2006
[cited by applicant]
JP 2009529926A
· 2009
[cited by applicant]
JP 2010095586A
· 2010
[cited by applicant]
KR 20020027747A
· 2002
[cited by applicant]
KR 20020032351A
· 2002
[cited by applicant]
KR 20030055102A
· 2003
[cited by applicant]
KR 101507301B1
· 2015
[cited by applicant]
KR 101985368B1
· 2019
[cited by applicant]
KR 102078334B1
· 2020
[cited by applicant]
WO 199739737A1
· 1997
[cited by applicant]
WO 1997039737A1
· 1997
[cited by applicant]
WO 2002064192A1
· 2002
[cited by applicant]
WO 2002085419A2
· 2002
[cited by applicant]
WO 2002092678A1
· 2002
[cited by applicant]
WO 2005020849A2
· 2005
[cited by applicant]
WO 2008127290A1
· 2008
[cited by applicant]
WO 2009108760A2
· 2010
[cited by applicant]
WO 2010146574A1
· 2010
[cited by applicant]
WO 2012048283A1
· 2012
[cited by applicant]
WO 2013174661A1
· 2013
[cited by applicant]
WO 2014093489A1
· 2014
[cited by applicant]
WO 2018056937A1
· 2018
[cited by applicant]
WO 2020051920A1
· 2020
[cited by applicant]
WO 2022025229A1
· 2022
[cited by applicant]
WO 2022038213A1
· 2022
[cited by applicant]
WO 2022265367A1
· 2022
[cited by applicant]
WO 2022265368A1
· 2022
[cited by applicant]
WO 2023019143A1
· 2023
[cited by applicant]
Kang (An Effect of Alginate on the Stability of LDH Nanosheets in Aqueous Solution and Preparation of Alginate/LDH Nanocompositions, 100 Carbohyd. Poly. 158 (Year: 2014).
[cited by examiner]
Jejurikar (A Novel Strategy for Preparing Mechanically Robust Ionically Cross-Linked Alginate Hydrogels, 6 Biomed. Mater. 025010 (Year: 2011).
[cited by examiner]
Gleghorn (Adhesive Properties of Laminated Alginate Gels for Tissue Engineering of Layered Structures, 85A J Biomed. Mater. Res. 611 (Year: 2008).
[cited by examiner]
Cho, W.J., S.H. Oh, and J.H. Lee, “Alginate Film as a Novel Post-Surgical Tissue Adhesion Barrier,” Journal of Biomaterials Science-Polymer, Edition 21, pp. 701-713, 2010, 14 pages total.
[cited by applicant]
Chang, J.-J., et al., “Electrospun anti-adhesion barrier made of chitosan alginate for reducing peritoneal adhesions,” Carbohydrate Polymers, 2012, 9 pages total.
[cited by applicant]
Hirasaki, Y., et al., “Development of a Novel Antiadhesive Material, Alginate Flakes, Ex Vivo and In Vivo,” Surgery Today, 2011, 8 pages total.
[cited by applicant]
Xu, J.B., J.P. Bartley, and R.A. Johnson, “Preparation and characterization of alginate hydrogel membranes crosslinked using a water-soluble carbodiimide,” Journal of Applied Polymer Science, 2003, 7 pages total.
[cited by applicant]
Oerther, S., et al., “Hyaluronate-alginate gel as a novel biomaterial: Mechanical properties and formation mechanism,” 1999, Biotechnology Bioeng, pp. 206-215.
[cited by applicant]
Oerther, S., et al., “High interaction alginate-hyaluronate associations by hyaluronate deacetylation for the preparation of efficient biomaterials,” 2000, Biopolymers, pp. 273-281.
[cited by applicant]
Ceana, H. Nezhat, et al., “Adhesion Prevention and Management,” Prevention & Management of Laparoendoscopic Surgical Complications, 3rd edition, Society of Laparoendoscopic Surgeons, 2011, 11 total pages.
[cited by applicant]
Zhang, Y., et al., “Thermosensitive methyl cellulose-based injectable hydrogels for post-operation anti-adhesion,” Jan. 30, 2014, 8 total pages.
[cited by applicant]
Wiseman, D.M., et al., “Metaanalysis of the safety and efficacy of an adhesion barrier (Interceed TC7) in laparotomy,” Apr. 1999, 3 total pages.
[cited by applicant]
Dania Al-Jaroudi, MD and Togas Tulandi MD et al., “Adhesion prevention in gynecologic surgery,” Aug. 2005, 9 total pages.
[cited by applicant]
Ten Broek, R.P., et al., “Efficacy of polyethylene glycol adhesion barrier after gynecological laparoscopic surgery: results of a randomized controlled pilot study,” 2012, 7 pages total.
[cited by applicant]
Ten Broek, R.P., et al., “Benefits and harms of adhesion barriers for abdominal surgery: a systematic review and meta-analysis,” The Lancet, Jan. 4, 2014, 12 pages total.
[cited by applicant]
Yang, B., et al., “Prevention of abdominal adhesion formation by thermosensitive PECE-hydrogel in a rat uterine horn model,” Jan. 2011, 10 pages total.
[cited by applicant]
Mettler, L., et al., “A safety and efficacy study of a resorbable hydrogel for reduction of post-operative adhesions following myomectomy,” May 2008, 8 pages total.
[cited by applicant]
Lauder, C.I., et al., “Use of a modified chitosan-dextran gel to prevent peritoneal adhesions in a porcine hemicolectomy model,” Aug. 2012, 7 pages total.
[cited by applicant]
Johns, D.A., et al., “Initial feasibility study of a sprayable hydrogel adhesion barrier system in patients undergoing laparoscopic ovarian surgery,” Aug. 2003, 5 pages total.
[cited by applicant]
Tjandra, J.J., et al., “A sprayable hydrogel adhesion barrier facilitates closure of defunctioning loop ileostomy: a randomized trial,” Jun. 2008, 5 pages total.
[cited by applicant]
Diamond, M.P., “Reduction of adhesions after uterine myomectomy by Seprafilm membrane (HAL-F): a blinded, prospective, randomized, multicenter clinical study; Seprafilm Adhesion Study Group,” Dec. 1996, 7 pages total.
[cited by applicant]
Falabella, C.A., et al., “Novel Macromolecular Crosslinking Hydrogel to Reduce Intra-Abdominal Adhesions,” Journal of Surgical Research, Apr. 2010, 7 total pages.
[cited by applicant]
Hill-West, J.L., et al., “Efficacy of adhesion barriers. Resorbable hydrogel, oxidized regenerated cellulose and hyaluronic acid,” Mar. 1996, 5 total pages.
[cited by applicant]
Becker, J.M., et al., “Prevention of postoperative abdominal adhesions by a sodium hyaluronate-based bioresorbable membrane: a prospective, randomized, double-blind multicenter study,” Oct. 1996, 2 total pages.
[cited by applicant]
Li, T.C., et al., “The value of an absorbable adhesion barrier, Interceed, in the prevention of adhesion reformation following microsurgical adhesiolysis,” Apr. 1994, 5 total pages.
[cited by applicant]
Luo, Y., et al., “Cross-linked hyaluronic acid hydrogel films: new biomaterials for drug delivery,” Journal of Controlled Release, Oct. 3, 2000, 16 total pages.
[cited by applicant]
Xiao Zheng Shu, et al., “In situ crosslinkable hyaluronan hydrogels for tissue engineering,” Biomaterials, Mar.-Apr. 2004, 10 total pages.
[cited by applicant]
Vrijland, W.W., et al., “Fewer intraperitoneal adhesions with use of hyaluronic acid-carboxymethylcellulose membrane: a randomized clinical trial,” Feb. 2002, 7 total pages.
[cited by applicant]
Bennett, S.L., et al., “Next-Generation HydroGel Films as Tissue Sealants and Adhesion Barriers,” Nov./Dec. 2003, 7 total pages.
[cited by applicant]
European Patent Office, Examination Report mailed Jul. 29, 2020 in European patent application No. 19 156 162.0, 9 pages total.
[cited by applicant]
F. Shen, et al., “A Study on The Fabrication of Porous Chitosan/Gelatin Network Scaffold For Tissue Engineering,” Polymer International, 2000, 4 pages total.
[cited by applicant]
Lindenhayn et al., “Retention of Hyaluronic Acid in Alginate Beads: Aspects for In Vitro Cartilage Engineering,” J. Biomed. Mater. Res., vol. 44:149-155, 1999.
[cited by applicant]
Parks et al., A Casting Based Process to Fabricate 3D Alginate Scaffolds and to Investigate the Influence of Heat.
[cited by applicant]
Patel et al., “Mucoadhesive Bilayer Tablets of Propranolol Hydrochloride,” AAPS PharmSciTech 83 Article 77, 2007.
[cited by applicant]
Peppas et al., “Hydrogels in Pharmaceutical Formulations,” EU J. Pharmaceutics and Biopharmaceutics, 50:27-46, 2000.
[cited by applicant]
Rivero et al., “Composite and bi-layer films based on gelatin and chitosan,” J. Food Eng., 90:531-539, 2009.
[cited by applicant]
Seidel, “Synthesis of PolyHEMA Hydrogels for Using as Biomaterials. Bulk and Solution Radical-Initiated Polymerization Techniques,” Mater. Res., vol. 3 No. 3, 2000.
[cited by applicant]
Shah et al., “Branching Morphogenesis and Kidney Disease,” Development 131:1449-1462, 2004.
[cited by applicant]
The et al., “Moisture barrier, wetting and mechanical properties of shellac/agar or shellac/cassava starch bilayer bio-membrane for food applications,” J. Membrane Sci., 325:277-283, 2008.
[cited by applicant]
Thu et al., “Gelatin enhances drug dispersion in alginate bilayer film via the formation of crystalline microaggregates,” International J. Pharmaceutics, 454:99-106, 2013.
[cited by applicant]
Transfer on Pore Architecture During Fabrication, Mat. Sci. Eng., vol. 1435, 2008.
[cited by applicant]
Tsang et al., “Fabrication of 30 Hepatic Tissues by Additive Photopatterning of Cellular Hydrogels,” FASEB J. 21:790-801, 2007.
[cited by applicant]
U.S. Patent and Trademark Office, “Dendritic Macroporous Hydrogels Prepared by Crystal Templating,” U.S. Appl. No. 12/919,667, filed Aug. 26, 2010, by Scott Zawko.
[cited by applicant]
U.S. Patent and Trademark Office, “One-Step Processing of Hydrogels for Mechanically Robust and Chemically Desired Features,” U.S. Appl. No. 13/269,366, filed Oct. 7, 2011, by Sarah Mayes.
[cited by applicant]
Uludag et al., “Technology of Mammalian Cell Encapsulation,” Advanced Drug Delivery Reviews, 29-64, 2000.
[cited by applicant]
Xu et al., “Biomimetic Mineralization,” J. Mater. Chem., 415-449, 2007.
[cited by applicant]
Yang et al., “The Design of Scaffolds for Use in Tissue Engineering. Part II. Rapid Prototyping Techniques,” Tissue Engineering, vol. 8 No. 1, 2002.
[cited by applicant]
Zawko et al., “Crystal Templating Dendritic Pore Networks and Fibrillar Microstructure into Hydrogels,” Acta Biomaterials, vol. 6:2415-2421, 2010.
[cited by applicant]
Zhang et al., “Pectin/lysozyme bilayers layer-by-layer deposited cellulose nanofibrous mats for antibacterial application,” Carbohydrate Polymers, 117:687-693, 2015.
[cited by applicant]
State Intellectual Property Office, People's Republic of China, First Office Action mailed Apr. 20, 2016 in Chinese Patent Application No. 201380072748.6.
[cited by applicant]
European Patent Office, Extended European Search Report mailed Jul. 4, 2016 in European Patent Application No. 13863368.0.
[cited by applicant]
Candian Patent Office, Examiner's Office Action mailed May 6, 2016 in Canadian Patent Application No. 2,894,658.
[cited by applicant]
IP Australia, “Patent Examination Report,” issued Oct. 28, 2015, in Australian application No. 2013359344.
[cited by applicant]
Kohane et al., “Polymeric Biomaterials in Tissue Engineering,” 63 Ped. RES., 487 (2008).
[cited by applicant]
Parks et al., A Casting Based Process to Fabricate 3D Alginate Scaffolds and to Investigate the Influence of Heat Transfer on Pore Architecture During Fabrication, 28 Mat. Sci. Eng. C 1435 (2008).
[cited by applicant]
Bekkers et al., “Targeted Dendrotomy Reveals Active and Passive Contributions of the Dendritic Tree to Synaptic Integration and Neuronal Output,” PNAS, Jul. 3, 2007, vol. 104 No. 27:11447-11452.
[cited by applicant]
Boulmedais et al., “Polyelectrolyte multilayer films with pegylated polypeptides as a new type of anti-microbial protection for biomaterials,” Biomaterials, 25 (2004), 2003-2011.
[cited by applicant]
Brisken et al., “Alveolar and Lactogenic Differentiation,” J. Mammary Gland Bio. Neoplasia, 2006, 11:239-248.
[cited by applicant]
Cabodi et al., “A Microfluidic Biomaterial,” JACS (J. Am. Chem. Soc.) Communications, Ithaca, New York, 2005, 2 pages.
[cited by applicant]
Chung, et al., “Effects of Auricular Chondrocyte Expansion on Neocartilage Formation in Photocrosslinked Hyaluronic Acid Networks,” Tissue Eng., Sep. 2006, 12(9) :2665-2673.
[cited by applicant]
Cook, et al., “Layer-by-layer coating of alginate matrices with chitosan-alginate for the improved survival and targeted delivery of probiotic bacteria after oral administration,” Journal of Materials Chemical, B 2013, …
[cited by applicant]
Depierro, “Influence of Polymerization Conditions on Nanostructure and Properties of Polyacrylamide Hydrogels Templated from Lyotropic Liquid Crystals,” Chemical Materials., Oct. 18, 2006, 5609-5617, vol. 18, No. 23, Am…
[cited by applicant]
Duffy, et al., “Rapid Prototyping of Microfluidic Systems in Polydimethylsiloxane,” Anal. Chem., 1998, 70:4974-4984.
[cited by applicant]
Fioramonti, et al., “Multilayer emulsions as a strategy for linseed oil microencapsulation: Effect of pH and alginate concentration,” Food Hydrocolloids, vol. 43, pp. 8-17, 2015.
[cited by applicant]
Gleghorn, et al., “Adhesive properties of laminated alginate gels for tissue engineering of layered structures,” Journal of Biomedical Material Res., 2008, 85A:611-618.
[cited by applicant]
Haidar et al., “Protein release kinetics for coreeshell hybrid nanoparticles based on the layer-by-layer assembly of alginate and chitosan on liposomes,” Biomaterials, 29:1207-1215, 2008.
[cited by applicant]
Huang, “Rapid Fabrication of 3D Branched Microvascular Flow Networks”, Twelfth International Conference on Miniaturized Systems for Chemistry and Life Sciences, 1435-1437, California, 2008.
[cited by applicant]
Huang, “Rapid Fabrication of Bio-inspired 3D Microfluidic Vascular Networks,” Advanced Mater., vol. 21:3567-3571, 2009.
[cited by applicant]
International Searching Authority, “Transmittal of the International Search Report and the Written Opinion of the International Searching Authority,” mailed Jan. 11, 2011 in International Application No. PCT/US2011/0554…
[cited by applicant]
International Searching Authority, “Transmittal of the International Search Report and the Written Opinion of the International Searching Authority,” mailed Dec. 1, 2011 in International Application No. PCT/US2011/05546…
[cited by applicant]
International Searching Authority, “Transmittal of the International Search Report and the Written Opinion of the International Searching Authority,” mailed Oct. 12, 2009 in International Application No. PCT/US2009/0352…
[cited by applicant]
International Searching Authority, “Supplemental Search Report” mailed Feb. 27, 2014 in International Application No. PCT/US2011/055469.
[cited by applicant]
Japanese Patent Office, “Notice of Reasons for Rejection,” Mailed on Jun. 30, 2015 in Japanese Application 2013-532995.
[cited by applicant]
Japanese Patent Office, “Final Rejection,” mailed on Mar. 29, 2016 in application 2013-532995.
[cited by applicant]
Jejurikar, et al., “A novel strategy for preparing mechanically robust ionically cross-linked alginate hydrogels,” BioMed. Mater. 6 (2011), 025010, 12 pp.
[cited by applicant]
Kang, et al., “An effect of alginate on the stability of LDH nanosheets in aqueous solution and preparation of alginate/LDH nanocomposites,” Carbohydrate Polymers, 100 (2014) 158-165.
[cited by applicant]
Khademhosseini et al., “Microscale Technologies for Tissue Engineering and Biology,” PNAS, vol. 103 No. 8:2480-2487, 2006.
[cited by applicant]
King et al., “Biodegradable Microfluidics,” Adv. Mater., vol. 16 No. 22:2007-2012, 2004.
[cited by applicant]
Kohane et al., “Polymeric Biomaterials in Tissue Engineering,” 63 Ped. Res., 2008.
[cited by applicant]
Larina et al., “Ca2+ Dynamics in Salivary Acinar Cells: Distinct Morphology of the Acinar Lumen Underlies Near-Synchronous Global Ca2+ Responses,” J. Cell. Science, 118:4131-4139.
[cited by applicant]
Leach et al., “Photocrosslinked Hyaluronic Acid Hydrogels: Natural, Biodegradable Tissue Engineering Scaffolds,” BioTech. BioEng., 82:578-259.
[cited by applicant]
Liverani, et al., “Simple fabrication technique for multilayered stratified composite scaffolds suitable for interface tissue engineering,” Materials Science & Engineering, A557 (2012) 54-58.
[cited by applicant]
Ma, et al., “Biodegradable Polymer Scaffolds with Well-Defined Interconnected Spherical Pore Network,” Tissue Engineering, vol. 7 No. 1: 23-39, 2001.
[cited by applicant]
Masters et al., “Designing Scaffolds for Valvular Interstitial Cells: Cell Adhesion and Function on Naturally Derived Materials,” J. Biomed. Mater Res., 71A:172-180, 2004.
[cited by applicant]
Miralles et al., “Sodium Alginate Sponges With or Without Sodium Hyaluronate: In Vitro Engineering of Cartilage,” J. Biomed. Mater. Res., vol. 57:268-278, 2001.
[cited by applicant]
Morch, et al., “Effect of Ca2+, Ba2+, and Sr2+ on Alginate Microbeads,” BioMacroMolecules, Italy, 2006, 1471-1480.
[cited by applicant]
Oaki et al., “Experimental Demonstration for the Morphological Evolution of Crystals Grown in Gel Media,” Crystal Growth & Design, vol. 3 No. 5:711-716, 2003.
[cited by applicant]
European Patent Office, Office Action mailed Mar. 19, 2021 in European Patent Application No. 11831723.9 (8 pages).
[cited by applicant]
European Patent Office, Communication pursuant to Article 94(3) mail Jun. 19, 2019 in European Patent Application No. 11831723.9.
[cited by applicant]
“Halobarrier Gel by Anika”, Anika.com, downloaded Sep. 6, 2023, https://anika.com/medical/products/surgical-solutions/#:~:text=Hyalobarrier%C2%AE%20Gel%20and%20Hyalobarrier,in%20the%20abdomino%2Dpelvic%20area.
[cited by applicant]
“SUPRO Adhesion Barrier Gel”, ansermedical.com, downloaded Sep. 6, 2023, https://www.ansermedical.com/products/adhesion-barrier-supro-gel.
[cited by applicant]
“An Absorbable Gel for Adhesion Prevention”, fziomed.com, downloaded Sep. 6, 2023, https://www.fziomed.com/products/peritoneal-surgery/.
[cited by applicant]
Froelich et al. “Alginate-Based Materials Loaded with Nanoparticles in Wound Healing”, Pharmaceutics, April 223; 15 (4): 1142, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10143535/.
[cited by applicant]
Bovone et al. “Engineering Hydrogel Adhesion for Biomedical Applications via Chemical Design of the Junction”, pubs.acs.org., ACS Biomater. Sci. Eng. 2021, 7, 9, 4048-4076, https://pubs.acs.org/doi/10.1021/acsbiomateria…
[cited by applicant]
Andersen et al. “In Situ Gelation for Cell Immobilization and Culture in Alginate Foam Scaffolds”, ncbi.nlm.nih.gov., Tissue Eng Part A., Feb. 1, 2014; 20(3-4): 600-610, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3926…
[cited by applicant]
Mayes, “Hyaluronic Acid and Alginate Blend Hydrogel Films for the Prevention of Postsurgical Adhesions”, The University of Texas at Austin, Doctoral Dissertation, May 2013 (246 pages).
[cited by applicant]