Granted Patent
B2
US 12,403,157 · App. 17/436,597 · Granted Sep 2, 2025
System and method to reduce tissue ororgan adhesion
Assignee:
Medcura, Inc.
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Abstract
A composition for providing a barrier between tissues and/or organs during or after a surgical procedure. The composition is a flowable solution of a hydrophobically-modified chitosan, wherein the hydrophobic modifications are selected to allow for adherence to tissue, cohesiveness of the composition upon application to tissue, and degradation over about two weeks after application. A method for providing a barrier between tissues and/or organs during or after a surgical procedure. Applying a hydrophobically-modified polymer to surfaces of tissues or organs in within a surgical site either before, during, and/or after a surgical procedure, wherein the modified polymer reduces adhesion between organs or tissues after surgery.
Claims (15)
1. A method for reducing adhesion between organs and tissues, comprising:
applying a flowable solution of a hydrophobically-modified chitosan, wherein hydrophobic modifications of chitosan are selected to allow for adherence to tissue, cohesiveness of the composition upon application to tissue, and degradation after application; wherein the hydrophobically-modified chitosan is applied to surfaces of tissues or organs within a surgical site either before, during, and/or after a surgical procedure, wherein the hydrophobically-modified chitosan reduces adhesion between organs or tissues after surgery; and wherein the hydrophobically modified chitosan has about 10% to 50% of available functional groups occupied by hydrophobic groups, and wherein the hydrophobic groups comprise a C1 to C4 hydrocarbon group and a hydrocarbon group of greater than C6.
2. The method of claim 1 , wherein the surfaces comprise parenchymal surfaces.
3. The method of claim 1 wherein the surgical procedure is performed in an animal.
4. The method of claim 1 , wherein the surgical procedure is performed in a human subject.
5. The method of claim 1 , wherein the degradation occurs within two weeks.
6. The method of claim 1 , wherein the degradation occurs within a period of two months, one month, one week, or within two days.
7. The method of claim 1 , wherein the C1 to C4 hydrocarbon group is present at 5:1 to 25:1 with respect to hydrocarbon groups of C6 or greater.
8. The method of claim 7 , comprising a hydrocarbon group of from C6 to C12 and a hydrocarbon group of from C16 or C18.
9. The method of claim 8 , comprising C8 hydrocarbons and C14, C16, and/or C18 hydrocarbons.
10. The method of claim 7 , wherein the hydrophobic groups comprise a C6 to C12 hydrocarbon and a C16 to C28 hydrocarbon.
11. The method of claim 10 , wherein the C6 to C12 hydrocarbons are present at a ratio of from 5:1 to 20:1 with respect to C16 to C28 hydrocarbons.
12. The method of claim 1 , wherein the hydrophobic groups comprise a C1 to C4 hydrocarbon group, a C6 to C12 hydrocarbon, and a C16 to C28 hydrocarbon.
13. The method of claim 1 , wherein the flowable solution is antibacterial.
14. The method of claim 1 , wherein the flowable solution creates an antibacterial barrier when applied to a tissue.
Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Feb 3, 2022
From: DOWLING, MATTHEW
To: GEL-E, INC.
Reel/Frame 058876/0761 →
Continuity (2)
Provisional Application
62814265
· Mar 5, 2019
References Cited (160)
US 4532134A
· Malette et al.
· 1985
[cited by applicant]
US 4572906A
· Sparkes et al.
· 1986
[cited by applicant]
US 4645757A
· Hjerten et al.
· 1987
[cited by applicant]
US 4752466A
· Saferstein et al.
· 1988
[cited by applicant]
US 4895724A
· Cardinal et al.
· 1990
[cited by applicant]
US 5243094A
· Borg
· 1993
[cited by applicant]
US 5426182A
· Jenkins et al.
· 1995
[cited by applicant]
US 5900479A
· Glasser et al.
· 1999
[cited by applicant]
US 5919574A
· Hoagland
· 1999
[cited by applicant]
US 6140089A
· Aebischer et al.
· 2000
[cited by applicant]
US 6371975B2
· Cruise et al.
· 2002
[cited by applicant]
US 6458147B1
· Cruise et al.
· 2002
[cited by applicant]
US 6548081B2
· Sadozai et al.
· 2003
[cited by applicant]
US 6602952B1
· Bentley et al.
· 2003
[cited by applicant]
US 6663653B2
· Akerfeldt
· 2003
[cited by applicant]
US 6827727B2
· Stalemark et al.
· 2004
[cited by applicant]
US 6830756B2
· Hnojewyj
· 2004
[cited by applicant]
US 6864245B2
· Vournakis et al.
· 2005
[cited by applicant]
US 6890344B2
· Levinson
· 2005
[cited by applicant]
US 6899889B1
· Hnojewyj et al.
· 2005
[cited by applicant]
US 6949114B2
· Milo et al.
· 2005
[cited by applicant]
US 6958325B2
· Domb
· 2005
[cited by applicant]
US 6994686B2
· Cruise et al.
· 2006
[cited by applicant]
US 6995137B2
· You et al.
· 2006
[cited by applicant]
US 7247314B2
· Hnojewyj et al.
· 2007
[cited by applicant]
US 7279001B2
· Addis et al.
· 2007
[cited by applicant]
US 7288532B1
· Payne et al.
· 2007
[cited by applicant]
US 7318933B2
· Hnojewyj
· 2008
[cited by applicant]
US 7351249B2
· Hnojewyj et al.
· 2008
[cited by applicant]
US 7482503B2
· Gregory et al.
· 2009
[cited by applicant]
US 7820872B2
· Gregory et al.
· 2010
[cited by applicant]
US 8664199B2
· Dowling et al.
· 2014
[cited by applicant]
US 8668899B2
· Dowling
· 2014
[cited by examiner]
US 20020028181A1
· Miller et al.
· 2002
[cited by applicant]
US 20020042473A1
· Trollsas et al.
· 2002
[cited by applicant]
US 20020068151A1
· Kim et al.
· 2002
[cited by applicant]
US 20040214736A1
· Modi
· 2004
[cited by applicant]
US 20050147656A1
· McCarthy et al.
· 2005
[cited by applicant]
US 20050181027A1
· Messinger
· 2005
[cited by applicant]
US 20060094060A1
· Jarhede et al.
· 2006
[cited by applicant]
US 20070148215A1
· Teslenko et al.
· 2007
[cited by applicant]
US 20100256671A1
· Falus
· 2010
[cited by applicant]
US 20190159992A1
· Dowling
· 2019
[cited by applicant]
DE 19629248A1
· 1997
[cited by applicant]
EP 1222926
· 2002
[cited by applicant]
FR 2933297A1
· 2010
[cited by applicant]
WO 1999002122A1
· 1999
[cited by applicant]
WO WO9903481A1
· 1999
[cited by examiner]
WO WO2018191705A1
· 2018
[cited by examiner]
Zhdanov et al. Adsorption and Spontaneous Rupture of Vesicles Composed of Two Types of Lipids (Langmuir 2006, 22, 3477-3480).
[cited by applicant]
Yoshina-Ishii et al., Diffusive Dynamics of Vesicles Tethered to a Fluid Supported Bilayer by Single-Particle Tracking, Langmuir 22(13):5682-5689 (2006).
[cited by applicant]
Yoshina-Ishii et al., General Method for Modification of Liposomes for Encoded Assembly on Supported Bilayers, J. Am. Chem. Soc. 127(5):1356-1357 (2005).
[cited by applicant]
Zhang, Jing. Drug Delivery: Self-Assembled Nanoparticles based on Hydrophobically Modified chitosan as Carriers for Doxorubicin, Nanomedicine, Elsevier. Aug. 2007. pp. 258-265.
[cited by applicant]
Kheirabadi, Bijan S. et al., Hemostatic Efficacy of Two Advanced Dressings in an Aortic Hemorrhage Model in Swine, J. Trauma Injury, Infection, and Critical Care, 59:25-35 (2005).
[cited by applicant]
Kim, Seung-Ho MD; Stezoski, S. William; Safar, Peter MD; Capone, Antonio MD; Tisherman, Samuel MD. “Hypothermia and Minimal Fluid Resuscitation Increase Survival after Uncontrolled Hemorrhagic Shock in Rats” Journal of …
[cited by applicant]
Kjoniksen et al., Light Scattering Study of Semidilute Aqueous Systems of Chitosan and Hydrophobically Modified Chitosans, Macromolecules 31(23):8142-8148 (1998).
[cited by applicant]
Knoll, W.; Frank, C. W.; Heibel, C.; Naumann, R.; Offenhausser, A.; Ruhe, J.; Schmidt, E. K.; Shen, W. W.; Sinner, A. “Functional tethered lipid bilayers.” J. Biotechnol. 2000, 74, 137-58.
[cited by applicant]
Koehler et al., Microstructure and Dynamics of Wormlike Micellar Solutions Formed by Mixing Cationic and Anionic Surfactants, J. Phys. Chem. B 104(47):11035-11044 (2000).
[cited by applicant]
Kozen, Buddy G et al., An Alternative Hemostatic Dressing: Comparison of CELOX, HemCon, and QuikClot, Acad. Emerg. Med. 15:74-81(2008).
[cited by applicant]
Kubota et al. Gelation dynamics and gel structure of fibrinogen. Colloids Surf B Biointerfaces 38:103-109, 2004.
[cited by applicant]
Kumar, R.; Raghavan, S. R. “Thermothickening in solutions of telechelic associating polymers and cyclodextrins.” Langmuir 2010, 26, 56-62.
[cited by applicant]
Kurth, Dirk G. and Thomas Bein. “Monomolecular Layers and Thin Films of Silane Coupling Agents by Vapor-Phase Adsorption on Oxidized Aluminum.” J. Phys. Chem. 1992. 96. 6707-6712.
[cited by applicant]
Larson, M. J.; Bowersox, J. C.; Lim, R. C.; Hess, J. R. “Efficacy of a fibrin hemostatic bandage in controlling hemorrhage from experimental arterial injuries.” Arch. Surg. 1995, 130, 420-422.
[cited by applicant]
Lee et al., Transition from Unilamellar to Bilamellar Vesicles Induced by an Amphiphilic Biopolymer, Phys. Review Letters, 96:048102-1-048102-4 (2006).
[cited by applicant]
Lee et al., Vesicle-Biopolymer Gels: Networks of Surfactant Vesicles Connected by Associating Biopolymers, Langmuir 21(1):26-33 (2005).
[cited by applicant]
Lew, W. K.; Weaver, F. A. “Clinical use of topical thrombin as a surgical hemostat.” Biologics 2008, 2, 593-599.
[cited by applicant]
Li et al., Multivesicular Liposomes for Oral Delivery of Recombinant Human Epidermal Growth Factor, Arch Pharm Res 28(8):988-994 (2005).
[cited by applicant]
Lu, S. et al. “Preparation of Water-Soluble Chitosan” Journal of Applied Polymer Science 91, 3497-2503 (2004).
[cited by applicant]
Lunelli et al., Covalently Anchored Lipid Structures on Amine-Enriched Polystyrene, Langmuir 21(18):8338-8343 (2005).
[cited by applicant]
Macfarlane, R. G. “An enzyme cascade in the blood dotting mechanism, and its function as a biological amplifier.” Nature 1964, 202, 498-499.
[cited by applicant]
Malette, William G. et al. Chitosan: A New Hemostatic, The Annals of Thoracic Surgery 36(1):55-58 (1983).
[cited by applicant]
Mansur Yalpani and Laurence D. Hall, Some Chemical and Analytical Aspects of Polysaccharide Modifications. Formation of Branched-Chain, Soluble Chitosan Derivatives, Macromolecules 17(3):272-281 (1984).
[cited by applicant]
Mathivet et al., Shape Change and Physical Properties of Giant Phospholipid Vesicles Prepared in the Presence of an AC Electric Field, Biophysical Journal 70:1112-1121 (1996).
[cited by applicant]
Meier, Wolfgang et al., Vesicle and Cell Networks: Interconnecting Cells by Synthetic Polymers, Langmuir 12:5028-5032 (1996).
[cited by applicant]
Michael I. Fisher and Torbjorn Tjarnhage, Structure and Activity of Lipid Membrane Biosensor Surfaces Studied with Atomic Force Microscopy and a Resonant Mirror, Biosensors & Bioelectronics 15:463-471 (2000).
[cited by applicant]
Muzzarelli, R et al., Antimicrobial Properties of N-Carboxybutyl Chtosan, Antimicrobial Agents and Chemotherapy, 34(10):2019-2023 (1990).
[cited by applicant]
Naumann et al., Proton Transport Through a Peptide-tethered Pilayer Lipid Membrane by the H.+-.ATP Synthase from Chloroplasts Measured by Impedance Spectroscopy, Biosensors and Bioelectronics 17:25-34 (2002).
[cited by applicant]
Naumann, C. A.; Prucker, O.; Lehmann, T.; Ruhe, J.; Knoll, W.; Frank, C. W. “The polymer-supported phospholipid bilayer: Tethering as a new approach to substrate-membrane stabilization.” Biomacromolecules2002, 3, 27-35.
[cited by applicant]
Neuffer, M. C.; McDivitt, J .; Rose, D.; King, K.; Cloonan, C. C.; Vayer, J. S. “Hemostatic dressings for the first responder: A review.” Military Med. 2004, 169, 716-720.
[cited by applicant]
New ! Pioneer Chip L1 Improved binding studies in model membrane systems, BIA Journal No. 2 1998.
[cited by applicant]
Nikolelis et al., A Minisensor for the Rapid Screening of Sucralose Based on Surface-stabilized Bilayer Lipid Membranes, Biosensors & Bioelectronics 15:439-444 (2000).
[cited by applicant]
Paul S. Cremer and Steven G. Boxer, Formation and Spreading of Lipid Bilayers on Planar Glass Supports, J. Phys. Chem. B 103(13):2554-2559 (1999).
[cited by applicant]
Wu et al., Spatially Selective Deposition of a Reactive Polysaccharide Layer onto a Patterned Template, Langmuir 19 (3):519-524 (2003).
[cited by applicant]
Pusateri, A. E.; Holcomb, J. B.; Kheirabadi, B. S.; Alam, H. B.; Wade, C. E.; Ryan, K. L. “Making sense of the preclinical literature on advanced hemostatic products.” J. Trauma 2006, 60, 674-682.
[cited by applicant]
Puu et al., Retained Activities of Some Membrane Proteins in Stable Lipid Bilayers on a Solid Support, Biosensors and Bioelectronics 10:463-476 (1995).
[cited by applicant]
Whang, Hyun Suk et al., Hemostatic Agents Derived from Chitin and Chitosan, J. Macromolecular Science 45:309-323 (2005).
[cited by applicant]
Raghavan, S. R.; Cipriano, B. H. Gel formation: Phase diagrams using tabletop rheology and calorimetry. InMolecular Gels; Weiss, R. G., Terech, P., Eds.; Springer: Dordrecht, 2005; pp. 233-244.
[cited by applicant]
Rao, S. B.; Sharma, C. P. “Use of chitosan as a biomaterial: Studies on its safety and hemostatic potential.” J. Biomed. Mater. Res. 1997, 34, 21-28.
[cited by applicant]
Tonelli, A. E. “Nanostructuring and functionalizing polymers with cyclodextrins.” Polymer 2008, 49, 1725-1736.
[cited by applicant]
Reiss, R. F.; Oz, M. C. “Autologous fibrin glue: Production and clinical use.” Transfusion Med. Rev. 1996, 10, 85-92.
[cited by applicant]
Rodriguez, M.S., et al.“Interaction between chitosan and oil under stomach and duodenal digestive chemical conditions” Biosci. Biotechnol. Biochem. 69 (11), 2057-2062 (2005).
[cited by applicant]
Rongen et al., Liposomes and Immunoassays, J. Immunol. Methods 204:105-133 (1997).
[cited by applicant]
Stavroula Sofou and James L. Thomas, Stable Adhesion of Phospholipid Vesicles to Modified Gold Surfaces, Biosensors and Bioelectronics 18:445-455 (2003).
[cited by applicant]
Stewart, R. M.; Myers, J. G.; Dent, D. L.; Ermis, P.; Gray, G. A.; Villarreal, R.; Blow, O.; Woods, B.; McFarland, M.; Garavaglia, J.; Root, H. D.; Pruitt, B. A. “Seven hundred fifty-three consecutive deaths in a level …
[cited by applicant]
Tanaka, M.; Sackmann, E. “Polymer-supported membranes as models of the cell surface.” Nature 2005,437, 656-663.
[cited by applicant]
Szymanska et al., Fullerene Modified Supported Lipid Membrane as Sensitive Element of Sensor for Odorants, Biosensors & Bioelectronics 16:911-915 (2001).
[cited by applicant]
Tanweer A. Khan and Kok Khiang Peh, A Preliminary Investigation of Chitosan Film as Dressing for Punch Biopsy Wound in Rats, J. Pharm. Pharmaceut. Sci. 6(1):20-26 (2003).
[cited by applicant]
Tangpasuthadol, Surface Modification of Chitosan Films. Effects of Hydrophobicity on Protein Adsorption, Carbohydrate Res. 338:937-942 (2003).
[cited by applicant]
Alam, Hasan B. et al., Comparative Analysis of Hemostatic Agents in a Swine Model of Lethal Groin Injury, J. Trauma 54:1077-1082 (2003).
[cited by applicant]
Allerbo et al., Simulation of lipid vesicle rupture induced by an adjacent supported lipid bilayer patch (Colloids and Surfaces B: Biointerfaces 2011, 82, 632-636).
[cited by applicant]
Anderluh et al., Properties of Nonfused Liposomes Immobilized on an L1 Biacore Chip and Their Permeabilization by a Eukaryotic Pore-forming Toxin, Anal. Biochem. 344:43-52 (2005).
[cited by applicant]
Angelova, M. I.; Dimitrov, D. S. “Liposome electroformation.” Faraday Discuss. 1986, 81, 303-306.
[cited by applicant]
Ankit R. Patel and Curtis W. Frank, Quantitative Analysis of Tethered Vesicle Assemblies by Quartz Crystal Microbalance with Dissipation Monitoring: Binding Dynamics and Bound Water Content, Langmuir 22(18):7587-7599 (2…
[cited by applicant]
Arnaud, F.; Teranishi, K.; Tomori, T.; Carr, W.; McCarron, R. “Comparison of 10 hemostatic dressings in a groin puncture model in swine.” J. Vascular Surg. 2009, 50, 632-639.
[cited by applicant]
Kheirabadi, B. S.; Scherer, M. R.; Estep, J. S.; Dubick, M. A.; Holcomb, J. B. “Determination of Efficacy of New Hemostatic Dressings in a Model of Extremity Arterial Hemorrhage in Swine.” J. Trauma 2009, 67, 450-460.
[cited by applicant]
Bochicchio, G .; Kilbourne, M .; Kuehn, R .; Keledjian, K .; Hess, J .; Scalea, T. “Use of a modified chitosan dressing in a hypothermic coagulopathic grade V liver injury model.” Am. J. Surg. 2009, 198, 617-622.
[cited by applicant]
Boukobza et al., Immobilization in Surface-Tethered Lipid Vesicles as a New Tool for Single Biomolecule Spectroscopy, J. Phys. Chem. B 105(48):12165-12170 (2001).
[cited by applicant]
Brandenberg, Greg et al., Chitosan: A New Topical Hemostatic Agent for Diffuse Capillary Bleeding in Brain Tissue, Neurosurgery 15(1): 9-13 (1984).
[cited by applicant]
Burkatovskaya, Marina et al., Use of Chitosan Bandage to Prevent Fatal Infections Developing From Highly Contaminated Wounds in Mice, Biomaterials 27:4157-4164 (2006).
[cited by applicant]
Champion, H. R.; Bellamy, R. F.; Roberts, C. P.; Leppaniemi, A. “A profile of combat injury.” J. Trauma2003, 54, S13-S19.
[cited by applicant]
Chenite, A. et al.“Rheological characterization of thermogelling chitosan/glycerol-phosphate solutions” Carbohydrate Polymers 46, 39-47 (2001).
[cited by applicant]
Chiaki Yoshina-Ishii and Steven G. Boxer, Arrays of Mobile Tethered Vesicles on Supported Lipid Bilayers, J. Am. Chem. Soc. 125(13):3696-3697 (2003).
[cited by applicant]
Christensen, S. M.; Stamou, D. “Surface-based lipid vesicle reactor systems: fabrication and applications.” Soft Matter 2007, 3, 828-836.
[cited by applicant]
Cooper et al., A Vesicle Capture Sensor Chip for Kinetic Analysis of Interactions with Membrane-Bound Receptors, Anal. Biochem. 277:196-205 (2000).
[cited by applicant]
Khan et al., Mechanical, Bioadhesive Strength and Biological Evaluations of Chitosan Films for Wound Dressing, J. Pharm. Pharmaceut. Sci. 3(3):303-311 (2000).
[cited by applicant]
D. D. Lasic and D. Papahadjopoulos, Liposomes Revisited, Science 267(5202): 1275-1276 (1995).
[cited by applicant]
Dan D. Lasic, Novel Applications of Liposomes, Trens in Biotechnology (TIBTECH) 16:307-321 (1998).
[cited by applicant]
Deng, Y.; Wang, Y.; Holtz, B.; Li, J. Y.; Traaseth, N.; Veglia, G.; Stottrup, B. J.; Elde, R.; Pei, D. Q.; Guo, A.; Zhu, X. Y. “Fluidic and air-stable supported lipid bilayer and cell-mimicking microarrays.” J. Am. Chem…
[cited by applicant]
Desbrieres et al., Hydrophobic Derivatives of Chitosan: Characterization and Rheological Behaviour, Biological Macromolecules, 19:21-28 (1996).
[cited by applicant]
Dimitrievski et al., Influence of Lipid-Bilayer-Associated Molecules on Lipid-Vesicle Adsorption (Langmuir 2010, 26 (8), 5706-5714).
[cited by applicant]
Dimitrievski et al., Simujlations of Lipid Vesicle Adsorption for Different Lipid mixtures (Langmuir 2008, 24, 4077-4091).
[cited by applicant]
Doolittle, R. F. “Fibrinogen and fibrin.” Annu. Rev. Biochem. 1984, 53, 195-229.
[cited by applicant]
Dowling, M.B., et al. “A self-assembling hydrophobically modified chitosan capable of reversible hemostatic action.” Biomaterials. May 2011 Vo. 31, pp. 3351-3357.
[cited by applicant]
Durian, Douglas J., et al. “Making a frothy shampoo or beer.” Physics Today. pp. 62-63. May 2010.
[cited by applicant]
Kean, T .; Thanou, M. “Biodegradation, biodistribution and toxicity of chitosan.” Adv. Drug Deliv. Rev. 2010,62, 3-11.
[cited by applicant]
Ellis-Behnke, R. G.; Liang, Y. X.; You, S. W.; Tay, D. K. C.; Zhang, S. G.; So, K. F.; Schneider, G. E. “Nano neuro knitting: Peptide nanofiber scaffold for brain repair and axon regeneration with functional return of v…
[cited by applicant]
Ellis-Behnke, R. G.; Liang, Y.- X.; Tay, D. K. C.; Kau, P. W. F.; Schneider, G. E.; Zhang, S.; Wu, W.; So, K.-F. “Nano hemostat solution: Immediate hemostasis at the nanoscale.” Nanomedicine 2006, 2, 207-215.
[cited by applicant]
Esquenet et al., Structural and Rheological Properties of Hydrophobically Modified Polysaccharide Associative Networks, Langmuir 20(9):3583-3592 (2004).
[cited by applicant]
F W Verheugt, M J van Eenige, J C Res, M L Simoons, P W Serruys, F Vermeer, D C van Hoogenhuyze, P J Remme, C de Zwaan, and F Baer. Bleeding complications of intracoronary fibrinolytic therapy in acute myocardial infarc…
[cited by applicant]
Fu et al., Protein stability in controlled-release systems, Nature Biotechnology 18:24-25 (2000).
[cited by applicant]
GlaxoSmithKline. Bactroban Ointment: Prescribing Information. Research Triangle Park, NC. May 2005. Downloaded from the world wide web on Jan. 17, 2013:.
[cited by applicant]
Gregory F. Payne and Srinivasa R. Raghavan, Chitosan: a Soft Interconnect for Hierarchical Assembly of Nano-scale Components, Soft Matter 3:521-527 (2007).
[cited by applicant]
Hirano and Noishiki, The Blood Compatibility of Chitosan and N-Acylchitosans, J. Biochem. Materials Res. 413-417 (1985).
[cited by applicant]
Kauvar, D. S.; Lefering R.; Wade, C. E. “Impact of hemorrhage on trauma outcome: An overview of epidemiology, clinical presentations, and therapeutic considerations.” J. Trauma 2006, 60, S3-S9.
[cited by applicant]
Ho et al. “Preparation and characterization of RGD-immobilized chitosan scaffolds”, Biomaterials 26 (2005) 3197-3206, published Oct. 14, 2004.
[cited by applicant]
Kaler et al., Phase Behavior and Structures of Mixtures of Anionic and Cationic Surfactants, J. Phys. Chem. 96(16): 6698-6707 (1992).
[cited by applicant]
Hong et al., Two-step Membrane Binding by Equinatoxin II, a Pore-forming Toxin from the Sea Anemone, Involves an Exposed Aromatic Cluster and a Flexible Helix, J. Biol. Chem. 277(44):41916-41924 (2002).
[cited by applicant]
Hook et al., Supported Lipid Bilayers, Tethered Lipid Vesicles, and Vesicle Fusion Investigated Using Gravimetric, Plasmonic, and Microscopy Techniques, Biointerphases 3(2) (Jun. 2008).
[cited by applicant]
Jung et al., Quantification of Tight Binding to Surface-Immobilized Phospholipid Vesicles Using Surface Plasmon Resonance: Binding Constant of Phospholipase A2, J. Am. Chem. Soc. 122(17):4177-4184 (2000).
[cited by applicant]