Granted Patent
B2
US 12,709,756 · App. 18/167,581 · Granted Aug 18, 2026
Plant cells, plants, and seeds having targeted enhancer element insertions
Inventors:
Yajie Niu (Lexington, MA); Randall William Shultz (Acton, MA); Maria Margarita D. Unson (Pawcatuck, CT); Michael Andreas Kock (Rheinfelden, DE); John Patrick Casey, Jr. (Boston, MA)
Assignee:
INARI AGRICULTURE TECHNOLOGY, INC.
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Abstract
The invention relates to novel plants, seeds and compositions, as well as improvements to plant breeding and methods for creating modifications in plant genomes.
Claims (13)
1 . A modified plant cell comprising a targeted modification in a first gene and a targeted modification in a second gene, wherein the targeted modifications result in an increase in expression of the first gene and the second gene relative to a plant cell lacking the modifications, wherein the targeted modifications comprise an insertion of an enhancer element consisting of SEQ ID NO: 343.
2 . The modified plant cell of claim 1 , wherein the targeted modifications comprise an insertion of the enhancer element in the promoter region of the first gene and the second gene.
3 . The modified plant cell of claim 1 , wherein the targeted modifications comprise an insertion of the enhancer element at about 50 to about 500 nucleotides upstream of the start codon of the first gene and the second gene.
4 . The modified plant cell of claim 1 , wherein the plant cell is a maize plant cell.
5 . The modified plant cell of claim 1 , wherein the plant cell is a soybean plant cell.
6 . A plant comprising the modified plant cell of claim 1 .
7 . The plant of claim 6 , wherein the plant is an inbred line or an F1 hybrid.
8 . A plant part comprising the modified plant cell of claim 1 , optionally wherein the plant part is a seed.
9 . A method of manufacturing commercial seed comprising growing the plant of claim 6 , and optionally further multiplying or propagating the plant, and using the plant to produce commercial seed.
10 . A method of manufacturing a processed plant product comprising processing the plant of claim 6 or a part thereof into the product.
11 . The method of claim 10 , wherein the product is seed meal, starch, sugar, silage, or oil.
12 . A processed plant product comprising the modified plant cell of claim 1 .
13 . The processed plant product of claim 12 , wherein the product is seed meal.
Continuity (12)
Provisional Application
62451708
· Jan 28, 2017
Provisional Application
62451710
· Jan 28, 2017
Provisional Application
62452610
· Jan 31, 2017
Provisional Application
62477244
· Mar 27, 2017
Provisional Application
62480989
· Apr 3, 2017
Provisional Application
62510645
· May 24, 2017
Provisional Application
62523675
· Jun 22, 2017
Provisional Application
62530495
· Jul 10, 2017
Provisional Application
62530839
· Jul 10, 2017
Provisional Application
62531305
· Jul 11, 2017
Related Publication
20230242927A1
· Aug 3, 2023
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