Artificial cell for single-cell mass spectrometry (SCMS) measurement and preparation method thereof
The present disclosure provides an artificial cell for single-cell mass spectrometry (SCMS) measurement and a preparation method thereof. In the present disclosure, the artificial cell includes an internal aqueous phase, an intermediate oil phase, and an external aqueous phase; where the internal aqueous phase includes polyethylene glycol (PEG) and a polyvinyl alcohol (PVA) aqueous solution, and the intermediate oil phase includes a chloroform-hexane mixture of L-α-phosphatidylcholine; and the external aqueous phase includes PVA and an F-68 aqueous solution. Compared with natural cell samples, new artificial single cells based on microfluidic self-assembly provided by the present disclosure have better uniformity, stability, and controllability. The artificial cell can effectively avoid significant measurement differences between individual single-cell samples, and effectively solve the problem of difficulty in stably preserving biological samples.
1 . An artificial cell for single-cell mass spectrometry (SCMS) measurement, comprising an internal aqueous phase, an intermediate oil phase, and an external aqueous phase; wherein the internal aqueous phase comprises polyethylene glycol (PEG) and a polyvinyl alcohol (PVA) aqueous solution, and the intermediate oil phase comprises a chloroform-hexane mixture of L-α-phosphatidylcholine; and the external aqueous phase comprises PVA and an F-68 aqueous solution.
2 . A preparation method of the artificial cell according to claim 1 , comprising the following steps:
S1, building a microfluidic platform for artificial cell preparation:
the microfluidic platform is a three-phase channel, comprising an internal aqueous phase channel, an oil phase channel, and an external aqueous phase channel; a size and a structure of the artificial cell, a speed of preparation, and a microstructure of the artificial cell are regulated by controlling a width, a flow rate, and a compound composition of the channels; and
S2, preparing a double-emulsion liposome-derived artificial cell based on microfluidic self-assembly:
substances are added into the three-phase channel and emulsion droplets are squeezed out through an aqueous solution to form a primary vesicle structure of an oil-water two-phase structure; and the primary vesicle structure automatically forms the artificial cell; wherein
in step S2, the PEG and the PVA aqueous solution are added into the internal aqueous phase channel;
the chloroform-hexane mixture of the L-α-phosphatidylcholine is added into the oil phase channel; and
the PVA and the F-68 aqueous solution are added into the external aqueous phase channel.
3 . The preparation method of the artificial cell according to claim 2 , wherein the internal aqueous phase channel is an intermediate channel; the oil phase channel is divided into two sub-channels located on two sides of the internal aqueous phase channel; and the external aqueous phase channel is divided into two sub-channels outside the internal aqueous phase channel.
4 . The preparation method of the artificial cell according to claim 2 , wherein an organic dye or a fluorescent dye is further added into the internal aqueous phase channel in step S2.
5 . The preparation method of the artificial cell according to claim 2 , wherein in step S2, target compounds of different contents are added into the internal aqueous phase channel; and the target compound comprises adenosine triphosphate (ATP).