Mirror based light sheet illumination system for light microscopy
Methods and systems for imaging a sample using fluorescence microscopy. High resolution lenses can be used for light sheet microscopy by tilting the excitation beam relative to the imaging optical axis. The excitation beam can be tilted using mirrors, instead of lenses, to generate the tilted illumination sheet. In some examples, a light path starts at a top downward-facing cone of light as it emerges from an optic fiber; a first off-axis parabolic mirror collimates in the x-axis, while a second off-axis parabolic mirror collimates in the y-axis, followed by the photomask to create four primary light sheets, which are then focused by a third off-axis parabolic mirror. As the four primary light sheets converge they create an interference pattern at an objective lens.
1 . A method for imaging a sample using fluorescence microscopy, the method comprising:
propagating a light beam into an illumination system comprising a plurality of mirrors, and using the plurality of mirrors to form a light sheet from the light beam such that a propagation axis of the light sheet is at an oblique angle relative to an imaging axis of an objective lens when the light sheet reaches a sample viewing area, wherein propagating the light beam comprises propagating the light beam onto a first off-axis parabolic mirror and collimating the light beam in a first axis;
illuminating a sample using the light sheet; and
imaging the sample through the objective lens.
2 . The method of claim 1 , wherein propagating the light beam comprises propagating the light beam onto a second off-axis parabolic mirror and collimating the light beam in a second axis.
3 . The method of claim 2 , wherein propagating the light beam comprises propagating the light beam onto a photomask and creating a plurality of primary light sheets.
4 . The method of claim 3 , wherein the photomask comprises a quadruple-slit photomask shaped for elongating the light sheet by creating an interference pattern.
5 . The method of claim 3 , wherein propagating the light beam comprises propagating the primary light sheets onto a third off-axis parabolic mirror, causing the primary light sheets to converge towards the objective lens.
6 . The method of claim 5 , wherein the third off-axis parabolic mirror is substantially identical to the second off-axis parabolic mirror, and wherein propagating the primary light sheets onto the third off-axis parabolic mirror comprises folding a light path back upon itself and creating a substantially uniform beam profile.
7 . The method of claim 1 , wherein propagating the light beam comprises originating the light beam from a collimated illuminator.
8 . The method of claim 7 , wherein the collimated illuminator comprises a laser source emitting a radially symmetric, Gaussian beam and a collimator.
9 . The method of claim 1 , wherein imaging the sample comprises observing the sample by eye or camera, using pre-existing light paths within a standard upright or inverted research-grade microscope.
10 . A method for imaging a sample using fluorescence microscopy, the method comprising:
propagating a light beam into an illumination system comprising a plurality of mirrors, and using the plurality of mirrors to form a light sheet from the light beam such that a propagation axis of the light sheet is at an oblique angle relative to an imaging axis of an objective lens when the light sheet reaches a sample viewing area;
illuminating a sample using the light sheet;
imaging the sample through the objective lens;
illuminating the sample using one or more additional light sheets converging on the sample from one or more different angles with respect to the sample; and
forming a moiré pattern on the sample using the light sheet and the one or more additional light sheets.
11 . A system for imaging a sample using fluorescence microscopy, the system comprising:
an objective lens;
a light source configured to propagate a light beam; and
an illumination system comprising a plurality of mirrors, wherein the illumination system is oriented for propagating the light beam by forming, using the plurality of mirrors, a light sheet from the light beam such that a propagation axis of the light sheet is at an oblique angle relative to an imaging axis of the objective lens when the light sheet reaches a sample viewing area, wherein propagating the light beam comprises propagating the light beam onto a first off-axis parabolic mirror and collimating the light beam in a first axis.
12 . The system of claim 11 , wherein propagating the light beam comprises propagating the light beam onto a second off-axis parabolic mirror and collimating the light beam in a second axis.
13 . The system of claim 12 , wherein propagating the light beam comprises propagating the light beam onto a photomask and creating a plurality of primary light sheets.
14 . The system of claim 13 , wherein the photomask comprises a quadruple-slit photomask shaped for elongating the light sheet by creating an interference pattern.
15 . The system of claim 13 , wherein propagating the light beam comprises propagating the primary light sheets onto a third off-axis parabolic mirror, causing the primary light sheets to converge towards the objective lens.
16 . The system of claim 15 , wherein the third off-axis parabolic mirror is substantially identical to the second off-axis parabolic mirror, and wherein propagating the primary light sheets onto the third off-axis parabolic mirror comprises folding a light path back upon itself and creating a substantially uniform beam profile.
17 . The system of claim 11 , wherein the light source comprises a collimated illuminator.
18 . The system of claim 17 , wherein the collimated illuminator comprises a laser source emitting a radially symmetric, Gaussian beam and a collimator.
19 . The system of claim 11 , comprising a camera configured for imaging the sample.
20 . A system for imaging a sample using fluorescence microscopy, the system comprising:
an objective lens;
a light source configured to propagate a light beam; and
an illumination system comprising a plurality of mirrors, wherein the illumination system is oriented for propagating the light beam by forming, using the plurality of mirrors, a light sheet from the light beam such that a propagation axis of the light sheet is at an oblique angle relative to an imaging axis of the objective lens when the light sheet reaches a sample viewing area;
one or more additional illuminators configured for illuminating the sample using one or more additional light sheets converging on the sample from one or more different angles with respect to the sample, wherein illuminating the sample using one or more additional light sheets comprises forming a moire pattern on the sample using the light sheet and the one or more additional light sheets.