Granted Patent
B2
US 12,735,688 · App. 18/335,481 · Granted Sep 15, 2026
Enzymes with RUVC domains
Inventors:
Brian Thomas (Emeryville, CA); Christopher Brown (Emeryville, CA); Rose Kantor (Emeryville, CA); Audra Devoto (Emeryville, CA); Cristina Butterfield (Emeryville, CA); Lisa Alexander (Emeryville, CA); Daniela S. A. Goltsman (Emeryville, CA); Jason Liu (Emeryville, CA)
Assignee:
Metagenomi Therapeutics, Inc.
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Abstract
The present disclosure provides for endonuclease enzymes having distinguishing domain features, as well as methods of using such enzymes or variants thereof.
Claims (6)
1 . An engineered nuclease composition comprising:
(a) an endonuclease comprising a RuvC_III domain and an HNH domain, wherein said RuvC_III domain comprises a sequence having at least 85% sequence identity to at least 21 consecutive amino acids of SEQ ID NO: 2917 and wherein said HNH domain comprises a sequence having at least 85% sequence identity to at least 20 consecutive amino acids of SEQ ID NO: 4731; and
(b) an engineered guide ribonucleic acid structure configured to form a complex with said endonuclease comprising:
(i) a guide ribonucleic acid sequence configured to hybridize to a target deoxyribonucleic acid sequence; and
(ii) a tracr ribonucleic acid sequence configured to bind to said endonuclease.
2 . The engineered nuclease composition of claim 1 , wherein said RuvC_III domain comprises a sequence having at least 90% sequence identity to at least 21 consecutive amino acids of SEQ ID NOs: 2917.
Assignments (4)
CHANGE OF NAME
Recorded Feb 6, 2026
From: METAGENOMI, INC.
To: METAGENOMI THERAPEUTICS, INC.
Reel/Frame 073717/0149 →
RELEASE OF SECURITY INTEREST
Recorded Dec 12, 2025
From: CATALIO NEXUS FUND III, LP
To: AFFINI-T THERAPEUTICS, INC.
Reel/Frame 073201/0168 →
SECURITY INTEREST
Recorded Mar 4, 2025
From: AFFINI-T THERAPEUTICS, INC.
To: CATALIO NEXUS FUND III, LP
Reel/Frame 070403/0862 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Oct 31, 2023
From: THOMAS, BRIAN; BROWN, CHRISTOPHER; KANTOR, ROSE; DEVOTO, AUDRA; BUTTERFIELD, CRISTINA; ALEXANDER, LISA; GOLTSMAN, DANIELA S.A.; LIU, JASON
To: METAGENOMI, INC.
Reel/Frame 065404/0028 →
Continuity (12)
Continuation
PCTUS2021031136
· May 6, 2021
Continuation
PCTUS2020018432
· Feb 14, 2020
Provisional Application
63180570
· Apr 27, 2021
Provisional Application
63116155
· Nov 19, 2020
Provisional Application
63032464
· May 29, 2020
Provisional Application
63022320
· May 8, 2020
Provisional Application
62874414
· Jul 15, 2019
Provisional Application
62805878
· Feb 14, 2019
Provisional Application
62805899
· Feb 14, 2019
Provisional Application
62805868
· Feb 14, 2019
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