Granted Patent
B2
US 12,735,689 · App. 19/344,313 · Granted Sep 15, 2026
Compositions and methods for nucleic acid modifications
Inventors:
David Rabuka (Berkeley, CA); Allison Sharrar (Berkeley, CA); Michael Schelle (Berkeley, CA); Luisa Mayumi Arake de Tacca (Berkeley, CA)
Assignee:
Acrigen Biosciences
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Abstract
The present disclosure provides nucleases and compositions, methods, and systems thereof for nucleic acid modification. More particularly, the present disclosure provides compositions and system comprising a nuclease comprising an amino acid sequence having at least 70% identity to any of SEQ ID NOs: 1-1096 and at least one gRNA.
Claims (8)
1 . A composition comprising a nuclease, wherein the nuclease comprises the amino acid sequence of SEQ ID NO: 535.
2 . The composition of claim 1 , further comprising a fusion protein comprising the nuclease.
3 . The composition of claim 2 , wherein the fusion protein comprises a nuclear localization sequence.
4 . A kit comprising the composition of claim 1 and at least one guide ribonucleic acid (gRNA).
5 . The kit of claim 4 , wherein the at least one gRNA comprises a first region configured to interact with the nuclease and a second region configured to hybridize with a portion of a target nucleic acid.
6 . An engineered nuclease, wherein the engineered nuclease comprises the amino acid sequence of SEQ ID NO: 535.
7 . A nucleic acid encoding the engineered nuclease of claim 6 .
8 . A vector comprising the nucleic acid of claim 7 .
Continuity (5)
Provisional Application
63249296
· Sep 28, 2021
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