PGC-1β, a novel PGC-1 homologue and uses therefor
The invention provides isolated nucleic acid molecules, designated PGC-1β nucleic acid molecules, which encode novel PGC-1 related coactivator molecules. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing PGC-1β nucleic acid molecules, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which a PGC-1β gene has been introduced or disrupted. The invention still further provides isolated PGC-1β proteins, fusion proteins, antigenic peptides and anti-PGC-1β antibodies. Diagnostic and therapeutic methods utilizing compositions of the invention are also provided.
1. An isolated polypeptide comprising an amino acid sequence which is at least 97% identical to the amino acid sequence of SEQ ID NO: 5, wherein the polypeptide modulates one or more of the following biological activities: activity of a nuclear receptor and/or nuclear respiratory factor 1 (NRF1); brown adipose cell determination and/or differentiation; mitochondrial activity and/or biogenesis; or fatty acid β-oxidation.
2. The isolated polypeptide of claim 1 comprising the amino acid sequence of SEQ ID NO: 5.
3. The polypeptide of claim 1 , further comprising a heterologous amino acid sequence.
4. An isolated polypeptide which is encoded by a nucleic acid molecule comprising a nucleotide sequence which is at least 97% identical to the nucleic acid sequence of SEQ ID NO: 4 or 6, wherein the polypeptide modulates one or more of the following biological activities: activity of a nuclear receptor and/or nuclear respiratory factor 1 (NRF1); brown adipose cell determination and/or differentiation; mitochondrial activity and/or biogenesis; or fatty acid β-oxidation.
5. The isolated polypeptide of claim 4 , further comprising a heterologous amino acid sequence.