Optical structures for metal-enhanced sensing
The present invention is directed to optical structures and methods detecting the fluorescence of a molecule using metal-enhanced fluorescence. In particular, the invention describes the use of surface plasmon excitation for excitation of fluorophores near the metal surface and the efficient collection of the emission by coupling into the plasmon resonance and directing towards the detector. More particularly, the present invention makes use of the unique directionality of the plasmon induced fluorescence signal. The present invention is directed to optical structures using metal enhanced fluorescence including: an optical fiber having a conductive external coating; a light emitting diode (LED) having a conical shaped depression with curved sides on a front end surface, the curved sides having a conducting coating on the outer surface with respect to the LED.
1. An optical structure for metal-enhanced fluorescence sensing, comprising:
an optical fiber having a conductive external coating;
fluorophores disposed adjacent to said conductive external coating, wherein said conductive external coating on said optical fiber comprises colloid spheres;
a detector disposed at one end of said optical fiber;
wherein fluorescence emissions of said fluorophores are coupled into said conductive external coating and into said optical fiber via application of surface excitation, and the fluorescence emissions are detected by the detector.
2. An optical structure for metal-enhanced fluorescence sensing according to claim 1 , wherein said fluorescence emissions coupled into said optical fiber are totally internally reflected within said optical fiber in a direction towards said detector, which detects said fluorescence emissions.
3. An optical structure for metal-enhanced fluorescence sensing according to claim 1 , further comprising:
a refractive index boundary being a surface of said optical fiber;
a first and second refractive index on either side of said refractive index boundary;
said optical fiber having said second refractive index, wherein said second refractive index is greater than said first refractive index; and
a stimulating light;
wherein, when an angle of incident of said fluorescence emission on said refractive index boundary is greater than a critical angle (θ c ), said fluorescence emission coupled into said optical fiber is totally internally reflected upon application of the stimulating light.
4. An optical structure for metal-enhanced fluorescence sensing according to claim 1 , wherein said conductive external coating on said optical fiber is a semi-transparent metal.
5. An optical structure for metal-enhanced fluorescence sensing according to claim 1 , wherein said conductive external coating on said optical fiber comprises a noble metal.
6. An optical structure for metal-enhanced fluorescence sensing, comprising:
an optical fiber having a conductive external coating;
fluorophores disposed adjacent to said conductive external coating, wherein said conductive external coating on said optical fiber comprises metallic islands;
a detector disposed at one end of said optical fiber;
wherein fluorescence emissions of said fluorophores are coupled into said conductive external coating and into said optical fiber via application of surface plasmon excitation, and the fluorescence emissions are detected by the detector.
7. A method of metal-enhanced fluorescence sensing, comprising:
applying a conductive coating to a surface of an optical fiber, wherein said conductive coating on said optical fiber comprises colloid spheres;
introducing a solution containing analytes to said conductive coating;
employing surface plasmon excitation to cause an excitation of fluorophores adjacent to said conductive coating;
coupling said fluorescence emissions of said fluorophores into said conductive coating and into said optical fiber, and wherein said fluorescence emissions are detected by a detector.
8. A method of metal-enhanced fluorescence sensing according to claim 7 , wherein said fluorescence emissions are totally internally reflected within said optical fiber in a direction towards the detector.
9. A method of metal-enhanced fluorescence sensing according to claim 7 , wherein said conductive coating on said optical fiber is a semi-transparent metal.
10. A method of metal-enhanced fluorescence sensing according to claim 7 , wherein said conductive coating on said optical fiber comprises a noble metal.
11. A method of metal-enhanced fluorescence sensing according to claim 8 comprising:
applying a conductive coating to a surface of an optical fiber, wherein said conductive coating on said optical fiber comprises metallic islands;
introducing a solution containing analytes to said conductive coating;
employing surface plasmon excitation to cause an excitation of fluorophores adjacent to said conductive coating;
coupling said fluorescence emissions of said fluorophores into said conductive coating and into said optical fiber, and the fluorescence emissions are detected by a detector.