Ribonucleic acid interference molecules and binding sites derived by analyzing intergenic and intronic regions of genomes
View Patent ↗In one aspect of the invention, a method for regulating the expression of a transcript comprises using at least one interfering RNA molecule that binds to an area of transcript containing a region that corresponds to at least one sequence having SEQ ID NO: 1, the interfering RNA molecule regulating the expression of the transcript through post-transcriptional silencing. In another aspect, a method for regulating the expression of a transcript comprises at least one of the provided sequences having SEQ ID NO: 1 being used to design an interfering RNA molecule that contains a region that corresponds to the reverse complement of one or more sequences having SEQ ID NO: 1, the interfering molecule regulating, through post-transcriptional silencing, transcripts that contain the sequence having SEQ ID NO: 1.
1. A method for decreasing the expression of a transcript, the method comprising the steps of:
identifying one or more regions of interest in a sequence of the given transcript;
sub-selecting, within the one or more identified regions of interest, a motif, wherein sub-selecting a motif comprises identifying one or more identically conserved copies of the motif in intergenic and intronic regions of a genome of interest for the given transcript and identifying one or more additional copies in untranslated and amino acid coding regions of one or more genes in the genome of interest, wherein sub-selecting a motif further comprises using a pattern discovery technique with a pre-determined parameter of minimum size of discovered pattern and a pre-determined parameter of minimum required number of discovered copies before a pattern can be deemed as identified;
generating one or more derived sequences from the sub-selected motif;
creating, from the one or more derived sequences, at least one instance of the corresponding molecule that the one or more derived sequences represent, wherein the at least one instance of the corresponding molecule comprises at least one interfering RNA molecule; and
using the at least one interfering RNA molecule to decrease the expression of the given transcript.