Granted Patent B2

US 8,515,683 · App. 13/079,719 · Granted Aug 20, 2013

Inventors: Abhijeet S. Gholap (San Jose, CA); Gauri A. Gholap (San Jose, CA); Chiruvolu V. K. Rao (Pune, IN); Sanford H. Barsky (Columbus, OH); Madhura Vipra (Pune, IN); Gurunath Kamble (Pune, IN); Suhas Manmantrao Patil (Pune, IN); Prithviraj Jadhav (Pune, IN)

Assignee: Ventana Medical Systems, Inc.

Abstract

Claims (20)

1. A computer software product that includes a non-transitory storage medium readable by a processor, the non-transitory storage medium having stored thereon a set of instructions for performing automated detection of immunohistochemical (IHC) patterns, the instructions comprising:

analyzing luminance values of plural pixels from a digital image of a human tissue sample to which an immunohistochemical (IHC) compound has been applied to segment the digital image into an IHC pattern area and background area;

identifying one or more probable epithelial cell areas in the segmented IHC pattern area;

detecting and filtering out stromal cell areas and lymph cell areas within the identified one or more probable epithelial cell areas to create one or more filtered probable epithelial cell area;

detecting and filtering out artifact areas from the one or more filtered probable epithelial cell areas; and

creating a medical conclusion using the one or more filtered probable epithelial cell areas.

2. The method of claim 1 further comprising a non-transitory computer readable medium having stored therein instructions for causing one or more processors to execute the steps of the method.

3. The method of claim 1 wherein the IHC compound includes a Haematoxylin and Eosin (HIE) stain.

4. The method of claim 1 wherein the medical conclusion includes a medical diagnosis or medical prognosis for a human cancer.

5. A system for performing automated detection of immunohistochemical (IHC) patterns, comprising:

a processor;

a computer readable memory coupled to the processor;

a network coupled to the processor;

software stored in the computer readable memory and executable by the processor, the software comprising:

means for performing steps of:

analyzing luminance values of plural pixels from a digital image of a human tissue sample to which an immunohistochemical (IHC) compound has been applied to segment the digital image into an IHC pattern area and background area;

identifying one or more probable epithelial cell areas in the segmented IHC pattern area;

detecting and filtering out stromal cell areas and lymph cell areas within the identified one or more probable epithelial cell areas to create one or more filtered probable epithelial cell area;

detecting and filtering out artifact areas from the one or more filtered probable epithelial cell areas; and

creating a medical conclusion using the one or more filtered probable epithelial cell areas.

Assignments (1)

Continuity (8)