Modular assay plates, reader systems and methods for test measurements
Luminescence test measurements are conducted using an assay module having integrated electrodes with a reader apparatus adapted to receive assay modules, induce luminescence, preferably electrode induced luminescence, in the wells or assay regions of the assay modules and measure the induced luminescence.
1. A method of
assembling a multi-well assay plate comprising introducing a multi-well assay module comprising an array of wells, into a plate frame that defines one or more apertures for receiving and holding one or more of said multi-well assay modules, wherein each assay module comprises:
(i) an array of wells with integrated electrodes,
(ii) a module lip,
(iii) a snap fit element extending from said module, said snap fit element positioned below said lip, wherein said lip and said snap fit element are configured to engage said plate frame,
(iv) a module top comprising a plurality of through-holes that form the walls of said array of wells, and
(v) a module bottom forming the bottom of said array of wells, and said plate frame further comprising a frame lip that extends into said one or more apertures, thereby engaging said module lip and said snap fit element within said plate frame,
wherein an assembled assay plate comprises said array of wells with integrated electrodes.
2. The method of claim 1 , wherein said multi-well assay module comprises assay reagents for conducting said electrode induced luminescence assay.
3. The method of claim 2 , wherein said assay reagents comprise binding reagents, labeled binding reagents, electrochemiluminescence (ECL) co-reactants, and combinations thereof.
4. The method of claim 3 , wherein said assay reagents are immobilized within one or more wells of said assay module.
5. The method of claim 1 , wherein said assembling step further comprises introducing an additional multi-well assay module comprising an additional array of wells into said plate frame, wherein said assembled plate comprises said array of wells and said additional array of wells with integrated electrodes.
6. The method of claim 5 , wherein said additional multi-well assay module comprises additional assay reagents used in said electrode induced luminescence assay.
7. The method of claim 6 , wherein said additional assay reagents comprise binding reagents, labeled binding reagents, electrochemiluminescence (ECL) co-reactants, and combinations thereof.
8. The method of claim 7 wherein said additional assay reagents are immobilized within one or more wells of said additional assay module.