Simian adenovirus nucleic acid and amino acid sequences, vectors containing same, and methods of use
A recombinant vector comprises simian adenovirus sequences and a heterologous gene under the control of regulatory sequences. A cell line which expresses simian adenovirus gene(s) is also disclosed. Methods of using the vectors and cell lines are provided.
1. A recombinant adenovirus having an adenovirus capsid comprising a SV25 hexon protein having the amino acid sequence of SEQ ID NO:34, said capsid having packaged therein a nucleic acid sequence comprising (a) an adenovirus 5′ inverted terminal repeat sequence, (b) a nucleotide sequence heterologous to SV25 encoding a therapeutic or immunogenic product operably linked to expression control sequences which direct transcription, translation, and/or expression thereof in a host cell, and (c) an adenovirus 3′ inverted terminal repeat sequence.
2. The recombinant adenovirus according to claim 1 , wherein the capsid further comprises a SV25 fiber protein.
3. The recombinant adenovirus according to claim 2 , wherein the adenovirus fiber protein has the sequence of SEQ ID NO:36.
4. The recombinant adenovirus according to claim 2 , wherein the adenovirus fiber protein has the sequence of SEQ ID NO: 35.
5. The recombinant adenovirus according to claim 1 , wherein the capsid further comprises a SV25 penton protein.
6. The recombinant adenovirus according to claim 5 , wherein the adenovirus SV25 penton protein has the sequence of SEQ ID NO: 33.
7. The recombinant adenovirus according to claim 1 , wherein the adenovirus capsid further comprises an adenovirus capsid protein heterologous to SV25.
8. The recombinant adenovirus according to claim 7 , wherein the heterologous adenovirus capsid protein is selected from one or more of (a) a penton protein, (b) a fiber protein, (c) a protein of the adenovirus L1 region selected from the 28.1 kD protein, polymerase, agnoprotein, 52/55 kD protein, and/or IIIa protein; (d) a protein of the adenovirus L2 region selected from the VII, VI, and/or Mu proteins; (e) a protein of the adenovirus L3 region selected from the VI and/or endoprotease; and (f) a protein of the adenovirus L4 region selected from the 100 kD protein, the 33 kD homolog, and/or VIII.
9. The recombinant adenovirus according to claim 1 , wherein the recombinant adenovirus is a pseudotyped adenovirus wherein the adenovirus 5′ inverted terminal repeat and the adenovirus 3′ inverted terminal repeat are heterologous to SV25.
10. The recombinant adenovirus according to claim 1 , wherein said adenovirus comprises minimal genomic adenovirus sequences which lacks all or a part of the E1 gene.
11. The recombinant adenovirus according to claim 1 , wherein said capsid further comprises one or more adenovirus capsid proteins selected from the group consisting of:
(a) a protein of the adenovirus L1 region selected from the 28.1 kD protein, polymerase, agnoprotein, 52/55 kD protein, and/or Ma protein of SV25;
(b) a protein of the adenovirus L2 region selected from the VII, VI, and/or Mu proteins of SV25;
(c) a protein of the adenovirus L3 region selected from the VI and/or endoprotease of SV25;
(d) a protein of the adenovirus L4 region selected from the 100 kD protein, the 33 kD homolog, and/or VIII of SV25.
12. A composition comprising the adenovirus according to claim 1 and a pharmaceutically acceptable carrier.