IP Library › Granted Patent US 9,441,201
Granted Patent B2
US 9,441,201 · App. 14/667,039 · Granted Sep 13, 2016

Native wharton's jelly stem cells and their purification

Inventor: Rouzbeh R. Taghizadeh (Cambridge, MA)
Assignee: Auxocell Laboratories, Inc.
C12N5/0662A61K35/51A61L27/3834C12N5/00C12N5/06C12N5/0605C12N2500/84C12N2502/025
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Quick Facts
Patent No.
US 9,441,201
App. No.
14/667,039
Granted
Sep 13, 2016
Kind
B2
Abstract

A method of culturing mesenchymal stem cells is provided. The mesenchymal stem cells are cultured in a medium that includes a cell-depleted Wharton's Jelly filtrate. The cell-depleted Wharton's Jelly filtrate is prepared by mincing umbilical cord tissue that includes Wharton's Jelly, subsequently diluting the umbilical cord tissue, subsequently filtering the umbilical cord tissue to generate a filtrate, and sedimenting Wharton's Jelly stem cells from the filtrate to generate the cell-depleted Wharton's Jelly filtrate.

Claims (3)

1. A method of culturing mesenchymal stem cells, the method comprising:

(a) culturing mesenchymal stem cells in a medium comprising a cell-depleted Wharton's Jelly filtrate,

wherein the cell-depleted Wharton's Jelly filtrate was prepared by mincing umbilical cord tissue comprising Wharton's Jelly, subsequently diluting the umbilical cord tissue, subsequently filtering the umbilical cord tissue to generate a filtrate, and sedimenting Wharton's Jelly stem cells from the filtrate to generate the cell-depleted Wharton's Jelly filtrate.

Assignments (3)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Oct 23, 2025
From: SLY, CHARLES DAVID; KORTH, ROY CHARLES
To: RKCS LLC
Reel/Frame 072652/0230 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jul 17, 2025
From: AUXOCELL LABORATORIES, INC.
To: SLY, CHARLES DAVID; KORTH, ROY CHARLES
Reel/Frame 071739/0557 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 19, 2016
From: TAGHIZADEH, ROUZBEH R.
To: AUXOCELL LABORATORIES, INC.
Reel/Frame 038321/0730 →
Continuity (4)
Continuation 13752928 · Jan 29, 2013
Continuation 13701329
Provisional Application 61350303 · Jun 1, 2010
Related Publication 20150197728A1 · Jul 16, 2015