Method for forming inorganic coatings
The present invention is directed to a method for forming an inorganic coating on a protein template. The method comprises contacting the template with an anionic polymer interface followed by an inorganic material for a sufficient period of time to allow mineralization of the inorganic material thus forming an inorganic coating on the template. Preferably, the coating is aligned.
1. A matrix comprising
an aligned inorganic coating on a protein template comprising silk fibroin,
wherein the protein template further comprises an anionic polymer,
wherein the anionic polymer interacts with the protein template so that a surface is established that promotes nucleation and growth of inorganic crystals,
wherein the anionic polymer comprises poly-L-aspartic acid and the aligned inorganic layer comprises hydroxyapatite, and
wherein the weight ratio of the poly-L-aspartic acid to the silk fibroin is between 200 mg/g and 1 g/g, inclusive.
2. The matrix of claim 1 , wherein the protein template further comprises at least one protein selected from keratins, collagens and silks.
3. The matrix of claim 1 , further comprising at least one additive.
4. A silk fibroin template comprising
an aligned inorganic coating, and
at least one therapeutic or biologically active agent,
wherein the silk fibroin template comprises an anionic polymer,
wherein the anionic polymer interacts with the silk fibroin template and promotes more regular nucleation and growth of inorganic crystals,
wherein the anionic polymer comprises poly-L-aspartic acid and the aligned inorganic layer comprises hydroxyapatite, and
wherein the weight ratio of the poly-L-aspartic acid to the silk fibroin is between 200 mg/g and 1 g/g, inclusive.
5. The silk fibroin template of claim 4 , wherein at least one therapeutic or biologically active agent is selected from the group consisting of: a small molecule, a protein, an antibody, a peptide, a nucleic acid, a polysaccharide, a glycoprotein, and a lipoprotein.
6. The silk fibroin template of claim 4 , wherein the at least one therapeutic or biologically active agent is a biologically active agent selected from the group consisting of cell attachment mediators, biologically active ligands, enzymes, and substances that either promote or inhibit cellular tissue ingrowth.
7. The silk fibroin template of claim 4 , wherein the at least one therapeutic or biologically active agent is a biologically active agent selected from the group consisting of collagen, elastin, fibronectin, vitronectin, laminin, proteoglycans, Arg-Gly-Asp (RGD) peptides, bone morphogenic proteins, cytokines, growth factor inhibitors, epidermal growth factor, fibroblast growth factor, platelet-derived growth factor, insulin-like growth factor, and transforming growth factor.
8. The silk fibroin template of claim 4 , wherein the at least one therapeutic or biologically active agent is incorporated in, or coated on, the aligned inorganic coating.
9. The silk fibroin template of claim 4 , wherein the at least one therapeutic or biologically active agent is incorporated in, or coated on, the silk fibroin template.
10. The silk fibroin template of claim 4 , wherein the silk fibroin template is selected from the group consisting of a silk fiber, a silk thread, a silk mesh, a silk film, a silk hydrogel, or a silk foam.
11. The silk fibroin template of claim 4 , wherein the silk fibroin template is a three-dimensional porous silk matrix.
12. The silk fibroin template of claim 4 , further comprising a pharmaceutically acceptable carrier.
13. The silk fibroin template of claim 4 , wherein the at least one therapeutic agent or biologically active agent is a therapeutic agent selected from the group consisting of anti-infectives, chemotherapeutic agents, anti-rejection agents, analgesics and analgesic combinations, anti-inflammatory agents, hormones, anti-angiogenic agents, anti-thrombotics, anti-metabolics, antimitotics, anticoagulants, fibrinolytics, growth factor inhibitors, growth factors, vitamins, sedatives, hypnotics, antibiotics, antiviral agents, steroids, growth factor promoters, prostaglandins, and radiopharmaceuticals.
14. The silk fibroin template of claim 13 , wherein the growth factor is selected from the group consisting of bone morphogenic proteins, bone morphogenic-like proteins, epidermal growth factor, fibroblast growth factor, platelet derived growth factor, insulin-like growth factor, transforming growth factors, and vascular endothelial growth factor.