Type II restriction modification system methylation subunit of
Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods for modulating or altering recombination inside or outside of a cell using isolated and/or purified polypeptides and/or nucleic acid sequences from Alicyclobacillus acidocaldarius.
1. A vector comprising a first nucleic acid sequence encoding a polypeptide having at least 90% sequence identity to SEQ ID NO:2874 and a second nucleic acid sequence heterologous to the first nucleic acid sequence.
2. The vector of claim 1 , wherein the polypeptide has activity at or below about pH 8.
3. The vector of claim 1 , wherein the polypeptide has activity at a temperature at or above about 50 degrees Celsius.
4. The vector of claim 1 , wherein the polypeptide has activity as a Type II restriction-modification system methylation subunit.
5. A vector comprising a first nucleic acid sequence encoding the polypeptide of SEQ ID NO:2874 and a second nucleic acid sequence heterologous to the first nucleic acid sequence.
6. The vector of claim 5 , wherein the first nucleic acid sequence comprises SEQ ID NO: 2875.
7. A method of modulating or altering nucleic acid methylation in a cell, the method comprising:
providing the vector of claim 1 to a cell; and
expressing the first nucleic acid sequence to produce the polypeptide.
8. The method according to claim 7 , wherein the modulation or alteration of nucleic acid methylation occurs at or below about pH 8.
9. The method according to claim 7 , wherein the modulation or alteration of nucleic acid methylation occurs at a temperature at or above about 50 degrees Celsius.
10. The method according to claim 7 , wherein the polypeptide is glycosylated, pegylated, or otherwise post-translationally modified.
11. The method according to claim 7 , wherein the polypeptide has activity as a Type II restriction-modification system methylation subunit.
12. The method according to claim 7 , wherein the polypeptide has activity at or below about pH 8.
13. The method according to claim 7 , wherein the polypeptide has activity at a temperature at or above about 50 degrees Celsius.
14. A method of modulating or altering nucleic acid methylation, the method comprising:
providing an isolated or purified polypeptide having at least 90% sequence identity to SEQ ID NO:2874 to a nucleotide sequence in which methylation is desired.
15. The method according to claim 14 , wherein the modulation or alteration of nucleic acid methylation occurs at or below about pH 8.
16. The method according to claim 14 , wherein the modulation or alteration of nucleic acid methylation occurs at a temperature at or above about 50 degrees Celsius.
17. The method according to claim 14 , wherein the purified or isolated polypeptide is glycosylated, pegylated, or otherwise post-translationally modified.
18. The method according to claim 14 , wherein the purified or isolated polypeptide has activity as a Type II restriction-modification system methylation subunit.
19. The method according to claim 14 , wherein the purified or isolated polypeptide has activity at or below about pH 8.
20. The method according to claim 14 , wherein the purified or isolated polypeptide has activity at a temperature at or above about 50 degrees Celsius.