IP Library › Granted Patent US 9,920,301
Granted Patent B2
US 9,920,301 · App. 15/393,999 · Granted Mar 20, 2018

Native Wharton's jelly stem cells and their purification

Inventor: Rouzbeh R. Taghizadeh (Cambridge, MA)
Assignee: Auxocell Laboratories, Inc.
C12N5/0668C12N5/00C12N5/06C12N5/0602C12N5/0605C12N5/0662C12N2500/84C12N2502/025
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Quick Facts
Patent No.
US 9,920,301
App. No.
15/393,999
Granted
Mar 20, 2018
Kind
B2
Abstract

Noncultured Wharton's Jelly stem cells and methods of their purification, storage and use are provided.

Claims (6)

1. A method of culturing mesenchymal stem cells, the method comprising:

(a) mincing umbilical cord tissue comprising Wharton's Jelly;

(b) subsequently diluting the umbilical cord tissue;

(c) subsequently filtering the umbilical cord tissue to generate a filtrate;

(d) sedimenting Wharton's Jelly stem cells from the filtrate to generate a cell-depleted Wharton's Jelly filtrate; and

(e) culturing mesenchymal stem cells in a medium comprising the cell-depleted Wharton's Jelly filtrate.

Assignments (3)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Oct 23, 2025
From: SLY, CHARLES DAVID; KORTH, ROY CHARLES
To: RKCS LLC
Reel/Frame 072652/0230 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jul 17, 2025
From: AUXOCELL LABORATORIES, INC.
To: SLY, CHARLES DAVID; KORTH, ROY CHARLES
Reel/Frame 071739/0557 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Dec 29, 2016
From: TAGHIZADEH, ROUZBEH R.
To: AUXOCELL LABORATORIES, INC.
Reel/Frame 040805/0241 →
Continuity (6)
Continuation 15241585 · Aug 19, 2016
Division 14667039 · Mar 24, 2015
Continuation 13752928 · Jan 29, 2013
Continuation 13701329
Provisional Application 61350303 · Jun 1, 2010
Related Publication 20170107496A1 · Apr 20, 2017