Chromatographic Media and Chromatographic Equipment Storage Solutions and Use Thereof
Buffered antimicrobial solutions of benzyl alcohol and ethanol and their use for short or long term storage of chromatographic solids.
1 . A buffered antimicrobial solution for storage of chromatographic solids, wherein the buffered anti-microbial solution comprises from about 1.5% to about 4% by weight benzyl alcohol, about 0.5% to about 4% by weight ethyl alcohol and from about 92% to about 98% by weight of about 50 mM to about 200 mM buffer to provide a solution at pH of about 5.5 to about 7.5.
2 . A buffered antimicrobial solution according to claim 1 wherein the pH is about pH 6.0.
3 . A buffered antimicrobial solution according to claim 1 wherein the amount of benzyl alcohol is about 2% by weight and the amount of ethyl alcohol is about 2% by weight.
4 . A buffered antimicrobial solution according to claim 1 wherein the buffer is selected from the group consisting of sodium citrate, sodium acetate, sodium phosphate, triethanolamine, TRIS (tris(hydromethyl)aminomethane), HEPES (N-(2-hydroxyethyl)piperizine-N′-(2-ethanesulphonate), and MES (4-morpholineethanesulphonate).
5 . A buffered antimicrobial solution according to claim 1 comprising about 2% by weight benzyl alcohol, about 2% by weight ethanol and about 96% by weight 100 mM sodium phosphate dibasic titrated to pH 6.0.
6 . A method of storing a chromatographic solid while preventing microbial infection of the solid comprising retaining the chromatographic solid in a buffered antimicrobial solution of claim 1 .
7 . A method of storing a chromatographic solid while preventing microbial infection of the solid comprising retaining the chromatographic solid in a buffered antimicrobial solution of claim 2 .
8 . A method of storing a chromatographic solid while preventing microbial infection of the solid comprising retaining the chromatographic solid in a buffered antimicrobial solution of claim 3 .
9 . A method of storing a chromatographic solid while preventing microbial infection of the solid comprising retaining the chromatographic solid in a buffered antimicrobial solution of claim 4 .
10 . A method of storing a chromatographic solid while preventing microbial infection of the solid comprising retaining the chromatographic solid in a buffered antimicrobial solution of claim 5 .
11 . A method according to claim 6 wherein the chromatographic solid is selected from chromatographic media or chromatographic equipment.
12 . A method according to claim 11 wherein the chromatographic solid is chromatographic media.
13 . A method according to claim 6 wherein the chromatographic solid is stored in the antimicrobial solution for a period of up to about two years.
14 . A method according to claim 10 wherein the chromatographic solid is stored in the buffered antimicrobial solution for a period of up to about two years.
15 . A method according to claim 13 wherein the chromatographic solid is chromatographic media.
16 . A method according to claim 14 wherein the chromatographic solid is chromatographic media.
17 . A chromatographic solid stored in a buffered antimicrobial solution, wherein the buffered antimicrobial solution comprises from about 1.5% to about 4% by weight benzyl alcohol, about 0.5% to about 4% by weight ethyl alcohol and from about 92% to about 98% by weight of about 50 mM to about 200 mM buffer to provide a solution at pH of about 5.5 to about 7.5.
18 . A chromatographic solid according to claim 17 wherein the chromatographic solid is selected from chromatographic media or chromatographic equipment.
19 . A chromatographic solid according to claim 17 wherein the chromatographic solid is stored in the buffered antimicrobial solution for a period of up to about two years.
20 . A chromatographic solid according to claim 17 wherein the buffered antimicrobial solution comprises about 2% by weight benzyl alcohol, about 2% by weight ethanol and about 96% by weight 100 mM sodium phosphate dibasic titrated to pH 6.0.