Delivery agent
A composition and method for delivering a peptide or protein into a cell comprising a polycationic agent and a polyanionic agent, wherein the polyanionic agent comprises an inorganic polyphosphate or a polyoxometalate and/or the polycationic agent comprises a polyalkyleneimine.
1. A method for delivering a peptide or protein into a target cell, the method comprising contacting the peptide or protein with a polycationic agent and a polyanionic agent to form a complex, and contacting the complex with the target cell to deliver the peptide or protein thereto, wherein
(1) the polyanionic agent comprises an inorganic polyphosphate or a polyoxometalate; and/or
(2) the polycationic agent comprises a polyalkyleneimine.
2. The method of claim 1 , wherein the polyanionic agent comprises a polyoxometalate.
3. The method of claim 2 , wherein the polyoxometalate comprises a molybdenum or tungsten polyoxometalate and optionally carries a heteroatom.
4. The method of claim 1 , wherein the polyanionic agent comprises a polyphosphate.
5. The method of claim 4 , wherein the polyphosphate comprises a nucleic acid or a synthetic analogue thereof.
6. The method of claim 1 , wherein the polycationic agent comprises a polyalkyleneimine.
7. The method of claim 1 , wherein the polycationic agent comprises a polyhydroxyalkyleneimine.
8. The method of claim 7 , wherein the polyhydroxyalkyleneimine is polyhydroxypropyleneimine.
9. The method of claim 6 , wherein the polyalkyleneimine comprises a polyethyleneimine.
10. The method of claim 1 , wherein the peptide or protein is contacted with the polyanionic agent prior to contacting the polycationic agent.
11. The method of claim 1 , wherein the peptide or protein comprises an enzyme, an antibody or a non-enzymatic, non-antibody protein.
12. The method of claim 1 , wherein the target cell is a suspension cell, an adherent cell, a primary cell or cultured cell.
13. A product comprising a peptide or protein, a polycationic agent, and a polyanionic agent as a combined preparation, wherein;
(1) the polyanionic agent comprises an inorganic polyphosphate or a polyoxometalate; and
(2) the polycationic agent comprises a polyalkyleneimine or a polyhydroxyalkyleneimine.