IP Library Granted Patent US 9,481,862
Granted Patent B2
US 9,481,862 · App. 14/147,028 · Granted Nov 1, 2016

Delivery agent

Inventors: Arunas Lagunavicius (Vilnius, LT); Lolita Zaliauskiene (Vilnius, LT)
Assignee: Thermo Fisher Scientific Baltics UAB
C12N5/0602A61K47/48015A61K47/48192A61K47/48223
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Quick Facts
Patent No.
US 9,481,862
App. No.
14/147,028
Granted
Nov 1, 2016
Kind
B2
Abstract

A composition and method for delivering a peptide or protein into a cell comprising a polycationic agent and a polyanionic agent, wherein the polyanionic agent comprises an inorganic polyphosphate or a polyoxometalate and/or the polycationic agent comprises a polyalkyleneimine.

Claims (17)

1. A method for delivering a peptide or protein into a target cell, the method comprising contacting the peptide or protein with a polycationic agent and a polyanionic agent to form a complex, and contacting the complex with the target cell to deliver the peptide or protein thereto, wherein

(1) the polyanionic agent comprises an inorganic polyphosphate or a polyoxometalate; and/or

(2) the polycationic agent comprises a polyalkyleneimine.

2. The method of claim 1 , wherein the polyanionic agent comprises a polyoxometalate.

3. The method of claim 2 , wherein the polyoxometalate comprises a molybdenum or tungsten polyoxometalate and optionally carries a heteroatom.

4. The method of claim 1 , wherein the polyanionic agent comprises a polyphosphate.

5. The method of claim 4 , wherein the polyphosphate comprises a nucleic acid or a synthetic analogue thereof.

6. The method of claim 1 , wherein the polycationic agent comprises a polyalkyleneimine.

7. The method of claim 1 , wherein the polycationic agent comprises a polyhydroxyalkyleneimine.

8. The method of claim 7 , wherein the polyhydroxyalkyleneimine is polyhydroxypropyleneimine.

9. The method of claim 6 , wherein the polyalkyleneimine comprises a polyethyleneimine.

10. The method of claim 1 , wherein the peptide or protein is contacted with the polyanionic agent prior to contacting the polycationic agent.

11. The method of claim 1 , wherein the peptide or protein comprises an enzyme, an antibody or a non-enzymatic, non-antibody protein.

12. The method of claim 1 , wherein the target cell is a suspension cell, an adherent cell, a primary cell or cultured cell.

13. A product comprising a peptide or protein, a polycationic agent, and a polyanionic agent as a combined preparation, wherein;

(1) the polyanionic agent comprises an inorganic polyphosphate or a polyoxometalate; and

(2) the polycationic agent comprises a polyalkyleneimine or a polyhydroxyalkyleneimine.

Priority Claims (1)
GB 0917792.4 · Oct 12, 2009 · national
Continuity (2)
Division 12901840 · Oct 11, 2010
Related Publication 20140120618A1 · May 1, 2014