Granted Patent B2

US 8,937,173 · App. 14/445,658 · Granted Jan 20, 2015

Inventors: Thomas H. Tuschl (New York, NY); Markus Landthaler (New York, NY); Gunter Meister (New York, NY); Sebastien Pfeffer (Strasbourg, FR)

Assignee: The Rockefeller University

C12N15/113C12N2310/141C12N2310/3521C12N2310/321

Abstract

Claims (19)

1. An isolated molecule comprising a maximum of fifty moieties, wherein each moiety comprises a base bonded to a backbone unit, said molecule comprising the microRNA molecule identified in SEQ ID NO: 144 or its corresponding anti-micro RNA molecule identified in SEQ ID NO: 450, wherein the molecule is modified for increased nuclease resistance.

2. The molecule according to claim 1 , wherein at least one of the moieties is a modified ribonucleotide moiety.

3. The molecule according to claim 2 , wherein the modified ribonucleotide is substituted at the 2′ position.

4. The molecule according to claim 3 , wherein the substituent at the 2′ position is a C 1 to C 4 alkyl group.

5. The molecule according to claim 4 , wherein the alkyl group is methyl.

6. The molecule according to claim 4 , wherein the alkyl group is allyl.

7. The molecule according to claim 4 , wherein the substituent at the 2′ position is a C 1 to C 4 alkoxy-C 1 to C 4 alkyl group.

8. The molecule according to claim 7 , wherein the C 1 to C 4 alkoxy-C 1 to C 4 alkyl group is methoxyethyl.

9. The molecule according to claim 1 , wherein at least one of the moieties is a 2′-fluororibonucleotide moiety.

10. The molecule according to claim 3 , wherein the modified ribonucleotide has a methylene bridge between the 2′-oxygen atom and the 4′-carbon atom.

11. The molecule according to claim 1 , wherein the molecule comprises at least one modified moiety on the 5′ end.

12. The molecule according to claim 1 , wherein the molecule comprises at least two modified moieties at the 5′ end.

13. The molecule according to claim 1 , wherein the molecule comprises at least one modified moiety on the 3′ end.

14. The molecule according to claim 1 , wherein the molecule comprises at least two modified moieties at the 3′ end.

15. The molecule according to claim 1 , wherein the molecule comprises at least two modified moieties at the 5′ end and at least two modified moieties at the 3′ end.

16. The molecule according to claim 1 , wherein the molecule comprises a nucleotide cap at the 5′ end, the 3′ end or both.

17. The molecule according to claim 1 , wherein the molecule consists of the microRNA molecule identified in SEQ ID NO: 144.

18. The molecule according to claim 1 , wherein the molecule consists of the anti-micro RNA molecule identified in SEQ ID NO: 450.

19. A vector comprising an isolated nucleic acid molecule according to claim 1 .

Assignments (1)

Continuity (8)