IP Library Granted Patent US 9,260,690
Granted Patent B2
US 9,260,690 · App. 14/542,841 · Granted Feb 16, 2016

System and method for electroporating a sample

Inventors: Richard Jarvis (Boerne, TX); Mike Byrom (Austin, TX); Dmitriy Ovcharenko (Austin, TX)
Assignee: Applied Biosystems, LLC
C12M35/02C12M45/07C12N15/86A61K48/00C12N2710/16632C12N2710/16643C12N2830/00C12N2830/008
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Quick Facts
Patent No.
US 9,260,690
App. No.
14/542,841
Granted
Feb 16, 2016
Kind
B2
Abstract

A system and method are described for electroporating a sample that utilizes one or more sets of electrodes that are spaced apart in order to hold a surface tension constrained sample between the electrodes. The first electrode is connected to the lower body of the system while the second electrode is connected to the upper body. Both electrodes are connected to a pulse generator. Each electrode has a sample contact surface such that the first electrode and the second electrode may be positioned to hold a surface tension constrained sample between the two sample contact surfaces and the sample may receive a selected electric pulse.

Claims (28)

1. An electrode array system for electroporation, comprising:

an electrode array;

a pulse generator electrically connected to the electrode array and operable to provide a pulsed electric current to the electrode array; and

a plurality of sample contact surfaces, each of the plurality of sample contact surfaces operable to hold an electroporation sample,

wherein the plurality of sample contact surfaces each include a conductive material to allow the pulsed electric current to pass from the electrode array and through the sample contact surface to the electroporation sample.

2. The system of claim 1 wherein the electrode array is configured to provide the pulsed electric current to ninety-six samples.

3. The system of claim 2 wherein the electrode array is configured to electroporate multiple samples simultaneously.

4. The system of claim 2 wherein the electrode array is configured to selectively electroporate one or more samples.

5. The system of claim 1 wherein the electrode array is configured to provide the pulsed electric current to eight samples.

6. The system of claim 5 wherein the electrode array is configured to electroporate multiple samples simultaneously.

7. The system of claim 5 wherein the electrode array is configured to selectively electroporate one or more samples.

8. The system of claim 1 further comprising at least one sample cuvette associated with the electrode array.

9. The system of claim 8 wherein the sample cuvette is configured to convey the pulsed electric current to a sample.

10. The systems of claim 1 wherein the electrode array is a reusable array.

11. The system of claim 1 wherein the electrode array is a disposable array.

12. An electrode array system for electroporation, comprising:

an electrode array including a plurality of individually controllable electrodes;

a pulse generator electrically connected to the electrode array and operable to selectively provide a pulsed electric current to each electrode of the electrode array; and

a plurality of sample contact surfaces, each of the plurality of sample contact surfaces operable to hold an electroporation sample,

wherein the plurality of sample contact surfaces each include a conductive material to allow the pulsed electric current to pass from the electrode array and through the sample contact surface to the electroporation sample.

13. The system of claim 12 wherein the electrode array is configured to provide the pulsed electric current to ninety-six electrodes.

14. The system of claim 13 wherein the electrode array is configured to electroporate multiple samples simultaneously.

15. The system of claim 12 wherein the electrode array is configured to provide the pulsed electric current to eight sample electrodes.

16. The system of claim 15 wherein the electrode array is configured to electroporate multiple samples simultaneously.

17. The system of claim 12 further comprising at least one sample cuvette associated with the electrode array.

18. The system of claim 17 wherein the sample cuvette is configured to convey the pulsed electric current to a sample.

19. The systems of claim 12 wherein the electrode array is a reusable array.

20. The system of claim 12 wherein the electrode array is a disposable array.

Assignments (4)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Dec 2, 2014
From: JARVIS, RICHARD A.; BYROM, MIKE W.; OVCHARENKO, DMITRIY
To: AMBION, INC.
Reel/Frame 034298/0895 →
MERGER Recorded Dec 2, 2014
From: AMBION, INC.
To: APPLERA CORPORATION
Reel/Frame 034299/0006 →
MERGER Recorded Dec 2, 2014
From: APPLERA CORPORATION
To: APPLIED BIOSYSTEMS INC.
Reel/Frame 034299/0163 →
MERGER Recorded Dec 2, 2014
From: APPLIED BIOSYSTEMS INC.
To: APPLIED BIOSYSTEMS, LLC
Reel/Frame 034299/0325 →
Continuity (6)
Continuation 13765671 · Feb 12, 2013
Continuation 12760526 · Apr 14, 2010
Continuation 12123619 · May 20, 2008
Division 11130884 · May 17, 2005
Continuation 10863102 · Jun 8, 2004
Related Publication 20150152374A1 · Jun 4, 2015