Nucleic acids encoding a cold- and menthol-sensitive receptor
The present invention relates to regulation of cold sensation and pain. More particularly, the present invention is directed to nucleic acids encoding a member of the transient regulatory protein family, CMR1, which is involved in modulation of the perception of cold sensations and pain. The invention further relates to methods for identifying and using agents that modulate cold responses and pain responses stimulated by cold via modulation of CMR1 and CMR1-related signal transduction.
1. A nucleic acid comprising an isolated cDNA or mRNA encoding a cold- and menthol-sensitive receptor (CMR1) polypeptide, wherein the nucleic acid encodes the amino acid sequence set forth in SEQ ID NO:3.
2. The isolated cDNA of claim 1 , which comprises the sequence of SEQ ID NO:4.
3. An expression vector comprising the cDNA or mRNA of claim 1 .
4. An expression vector comprising the cDNA of claim 2 .
5. An isolated host cell comprising the vector of claim 3 .
6. An isolated host cell comprising the vector of claim 4 .
7. The host cell of claim 5 which is a mammalian, yeast or insect cell.
8. The host cell of claim 6 which is a mammalian, yeast or insect cell.
9. The host cell of claim 5 which is a CHO or 293 cell.
10. The host cell of claim 6 which is a CHO or 293 cell.