Delivery agent
A composition comprising a polycationic agent and a polyanionic agent, and kits comprising the composition, is provided. In embodiments, the polyanionic agent is a nucleic acid and the polycationic agent is a modified polyalkyleneimine polymer.
1. A composition for delivering a peptide or protein into a cell, which composition comprises a polycationic agent and a polyanionic agent, wherein the polyanionic agent is a nucleic acid and the polycationic agent is a modified polyalkyleneimine polymer having a repeat unit of formula I or formula V:
where:
Z1 and Z2 each independently represent —H, alkyl or a branching chain;
Y1 and Y2 each independently represent a group having formula II or III:
where:
X1 is selected from the group consisting of —H, -alkyl, —SH, —CN, —NH 2 , —N(alkyl) 2 ,—NH(alkyl), —CONH 2 -,
—NHCONH 2 ,
X2 is selected from the group consisting of —CH 2 —, —O—, —S—, —NH—, or —N(alkyl)-;
provided that at least one of X1 and X2 is a hydrophilic group;
m1, m2, n1 and n2 each independently is 0, 1, 2or 3; and
p1 and p2 each independently is 1 or 2.
2. The composition of claim 1 , wherein the nucleic acid is DNA.
3. A kit for delivering a peptide or protein into a cell, which kit comprises a polycationic agent in a first container and a polyanionic agent in a second container, wherein the polyanionic agent is a nucleic acid and the polycationic agent is a modified polyalkyleneimine polymer having a repeat unit of formula I or formula V:
where:
Z1and Z2 each independently represent —H, alkyl or a branching chain;
Y1 and Y2 each independently represent a group having formula II or III:
where:
X1 is selected from the group consisting of —H, -alkyl, —SH, —CN, —NH 2 , —N(alkyl) 2 ,—NH(alkyl), —CONH 2 -,
CONH 2 , —NHCONH 2 ,
X2is selected from the group consisting of —CH 2 —, —O—, —S—, —NH—, or —N(alkyl)-;
provided that at least one of X1 and X2 is a hydrophilic group;
m1, m2, n1 and n2 each independently is 0, 1, 2or 3; and
p1 and p2 each independently is 1or 2.
4. The kit of claim 3 where the nucleic acid is DNA.
5. A method for delivering a peptide or protein into a target cell, which method comprises contacting the peptide or protein with a polycationic agent and a polyanionic agent to form a complex and contacting the complex with the target cell so as to deliver the peptide or protein thereto, wherein:
(1) the polyanionic agent is a nucleic acid; and
(2) the polycationic agent is a modified polyalkyleneimine polymer having a repeat unit of formula I or formula V:
where:
Z1 and Z2 each independently represent —H, alkyl or a branching chain;
Y1 and Y2 each independently represent a group having formula II or III:
where:
X1 is selected from the group consisting of —H, -alkyl, —SH, —CN, —NH 2 , —N(alkyl) 2 , —NH(alkyl), —CONH 2 ,
—NHCONH 2 ,
X2is selected from the group consisting of —CH 2 —, —O—, —S—, —NH—, or —N(alkyl)-;
provided that at least one of X1 and X2 is a hydrophilic group;
m1, m2, n1and n2 each independently is 0, 1, 2 or 3; and
p1 and p2 each independently is 1or 2.
6. The method according to claim 5 , wherein the peptide or protein is contacted with the polyanionic agent prior to contacting the polycationic agent.
7. The method according to claim 5 , wherein the peptide or protein comprises an enzyme, an antibody or an inert protein.
8. The method according to claim 5 , wherein the target cell is a suspension cell, an adherent cell, a primary cell or cultured cell.
9. The method of claim 5 where the nucleic acid is DNA.