Magnetic lysis method and device
A method for lysing cells is disclosed. The method includes stirring cells with a magnetic stir element in the presence of a plurality of cell lysis beads at a speed sufficient to lyse the cells. Also disclosed is a device for lysing cells. The device includes a container having a magnetic stir element and a plurality of cell lysis beads disposed therein. The container is dimensioned to allow rotation of the magnetic stir element inside the container.
1. A device for lysing cells, comprising:
a chamber, wherein inside of the chamber contains a magnetic stir element and a plurality of beads, wherein the chamber is dimensioned to allow rotation of the magnetic stir element inside the chamber and wherein the chamber is placed on a surface; and
a magnet in the proximity of the chamber and not below the chamber, wherein the magnet is located above the bottom of the chamber and produces a varying magnetic field by rotating the magnet along an axis that is located above the bottom of the chamber and is parallel to the surface the chamber is placed on or the magnet is located on a side of the chamber, and produces a varying magnetic field by rotating the magnet along an axis that is located on the side of the chamber, wherein the axis passes through the center of the magnet, and wherein the chamber is in operational range of the varying magnetic field such that motions of the magnet stir element and the plurality of beads inside of the chamber are driven by the varying magnetic field.
2. The device of claim 1 , further comprising a temperature control module that controls the temperature of the chamber.
3. The device of claim 1 , wherein the plurality of heads comprise a material selected from the group consisting of plastics, glass, ceramics, metals and mixtures thereof.
4. The device of claim 1 , wherein the plurality of beads comprise silica beads.
5. The device of claim 1 , wherein the plurality of beads have diameters within the range of 10-1000 μm.
6. The device of claim 1 , wherein the magnetic stirrer element comprises a metal or an alloy.
7. The device of claim 1 , wherein the magnetic stirrer element comprises an alloy core coated with a polymer.
8. The device of claim 7 , wherein the alloy core comprises neodymium iron boron or samarium cobalt and/or wherein the polymer is a biocompatible polymer, optionally polytetrafluoroethylene (PTFE) or parylene.